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Ramezani N, Vanaky B, Shakeri N, Soltanian Z, Fakhari Rad F, Shams Z. Evaluation of Bcl-2 and Bax Expression in the Heart of Diabetic Rats after Four Weeks of High Intensity Interval Training. mljgoums 2019; 13 (1) :15-20
URL: http://mlj.goums.ac.ir/article-1-1149-en.html
URL: http://mlj.goums.ac.ir/article-1-1149-en.html
Nasrin Ramezani 
1, Behnaz Vanaky2 , Nader Shakeri2 , Zahra Soltanian3 , Fatemeh Fakhari Rad3 , Zahra Shams4
1, Behnaz Vanaky2 , Nader Shakeri2 , Zahra Soltanian3 , Fatemeh Fakhari Rad3 , Zahra Shams4
1- Department of Sport Physiology, Faculty of Physical Education and Sport Sciences, Tehran University, Tehran, Iran
2- Department of Physical Education and Sport Sciences, Sciences and Research Branch, Islamic Azad University, Tehran, Iran
3- Department of Sport Physiology, Department of Physical Education and Sport Sciences, Sciences and Research Branch, Islamic Azad University, Tehran, Iran
4- Department of Sport Physiology, Faculty of Physical Education and Sports science, Central Tehran Branch, Islamic Azad University, Tehran, Iran
2- Department of Physical Education and Sport Sciences, Sciences and Research Branch, Islamic Azad University, Tehran, Iran
3- Department of Sport Physiology, Department of Physical Education and Sport Sciences, Sciences and Research Branch, Islamic Azad University, Tehran, Iran
4- Department of Sport Physiology, Faculty of Physical Education and Sports science, Central Tehran Branch, Islamic Azad University, Tehran, Iran
Abstract: (8928 Views)
ABSTRACT
Background and Objectives: Diabetes is one of the most common diseases and a major risk factor for cardiovascular disease. Studies have shown that regular exercise can affect apoptosis in cardiomyocytes. The purpose of this study was to investigate the effect of high-intensity interval training on the expression of Bcl-2 and Bax as important apoptosis factors in diabetic rats.
Methods: After inducing diabetes in 20 male Wistar rats (weighing 250 ± 1.15 g), the rats were randomly divided into a control group and a training group. The training group performed high-intensity interval training five days a week for four weeks, and the control group did not perform any training. After the intervention, RNA was extracted and TCF mRNA was subjected to real time RT-PCR for measuring Bax and Bcl-2 expression in the heart tissue of diabetic rats. Data were analyzed by IBM SPSS Statistics V22 using independent t-test. P-values less than 0.05 were considered as statistically significant.
Results: The expression of Bcl-2 increased significantly and the expression of Bax decreased significantly after the four-week training intervention.
Conclusion: The high-intensity interval training can have beneficial effects on the expression of apoptotic genes in rats with type 2 diabetes.
Keywords: Bax, Bcl-2, High intensity interval training, Heart, Type 2 diabetes.
Background and Objectives: Diabetes is one of the most common diseases and a major risk factor for cardiovascular disease. Studies have shown that regular exercise can affect apoptosis in cardiomyocytes. The purpose of this study was to investigate the effect of high-intensity interval training on the expression of Bcl-2 and Bax as important apoptosis factors in diabetic rats.
Methods: After inducing diabetes in 20 male Wistar rats (weighing 250 ± 1.15 g), the rats were randomly divided into a control group and a training group. The training group performed high-intensity interval training five days a week for four weeks, and the control group did not perform any training. After the intervention, RNA was extracted and TCF mRNA was subjected to real time RT-PCR for measuring Bax and Bcl-2 expression in the heart tissue of diabetic rats. Data were analyzed by IBM SPSS Statistics V22 using independent t-test. P-values less than 0.05 were considered as statistically significant.
Results: The expression of Bcl-2 increased significantly and the expression of Bax decreased significantly after the four-week training intervention.
Conclusion: The high-intensity interval training can have beneficial effects on the expression of apoptotic genes in rats with type 2 diabetes.
Keywords: Bax, Bcl-2, High intensity interval training, Heart, Type 2 diabetes.
Research Article: Original Paper |
Subject:
Human Genetics
Received: 2018/12/2 | Accepted: 2018/12/2 | Published: 2018/12/2 | ePublished: 2018/12/2
Received: 2018/12/2 | Accepted: 2018/12/2 | Published: 2018/12/2 | ePublished: 2018/12/2
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