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mljgoums 2019, 13(5): 44-49 |
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ramezani S, peeri M, Azarbaijani M A, Dehghan F. Combined Effects of Regular Aerobic Exercise with Vitamin D Supplementation on Some Apoptosis Indices in the Lung of Rats Exposed to Hydrogen Peroxide. mljgoums 2019; 13 (5) :44-49
URL: http://mlj.goums.ac.ir/article-1-1190-en.html
URL: http://mlj.goums.ac.ir/article-1-1190-en.html
1- Dept. of Exercise Physiology, School of Physical Education and Sport Sciences, Central Tehran
2- Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran,IRAN ,m.peeri@iauctb.ac.ir
3- physical education-azad university-Tehran-iran
4- Professor of Tehran University, Physiology
2- Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran,IRAN ,
3- physical education-azad university-Tehran-iran
4- Professor of Tehran University, Physiology
Abstract: (5113 Views)
ABSTRACT
Background and Objectives: Exercise is a strong physiological stimulus that can affect apoptosis in the lungs by altering a number of extracellular and intracellular signaling pathways. The present study examined effects of regular aerobic exercise and vitamin D on expression of Bcl-2, Bax and caspase-3 in lung tissues of male rats exposed to hydrogen peroxide.
Methods: Forty-eight adult male Wistar rats were randomly assigned into six groups of eight, including 2 x H 2 O 2, 2H 2 O 2, Vit D (2HD), 2H2O2 + Regular Exercise Training (2HE), 2H2O2 + D3 + E (2HDE) ; Dimethyl sulfoxide (DMSO) and control (C). Subjects in the training groups performed aerobic exercise for eight weeks. Bax, Bcl-2, caspase-3 expression in the lung tissues was measured using RT-PCR.
Results: Bcl-2 expression in the exercise (P = 0.004) and vitamin D (P = 0.006) groups increased significantly compared to the control groups. Bax, Bcl-2 and caspase-3 expression was significantly lower in the exercise group and vitamin D supplementation group compared to the control group. On the other hand, concurrent exercise and vitamin D significantly reduce Bax expression but had no significant effect on Bcl-2 and caspase-3 expression.
Conclusion: Our results demonstrate that regular aerobic exercise along with vitamin D supplementation may play a role in reducing apoptosis in lungs following severe oxidative stress.
Keywords: Apoptosis, Bcl-2, Bax, Caspase3, Aerobic Exercise, Vitamin D
Research Article: Original Paper |
Subject:
Sport Physiology
Received: 2019/02/6 | Accepted: 2019/02/16 | Published: 2019/09/2 | ePublished: 2019/09/2
Received: 2019/02/6 | Accepted: 2019/02/16 | Published: 2019/09/2 | ePublished: 2019/09/2
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.