Fri, Nov 22, 2024
Volume 16, Issue 3 (May-Jun 2022)
mljgoums 2022, 16(3): 30-35 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
zojaji Z, farajtabar behrestaq S, askari B. Effect of Eight Weeks of High Intensity Interval Training on Insulin Resistance and IRS1 Gene Expression in Gastrocnemius Muscle of Obese Wistar Rats. mljgoums 2022; 16 (3) :30-35
URL: http://mlj.goums.ac.ir/article-1-1312-en.html
URL: http://mlj.goums.ac.ir/article-1-1312-en.html
1- Department of Physical Education and Sport Sciences, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran
2- Department of Physical Education and Sport Sciences, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran ,farajtabarp@yahoo.com
2- Department of Physical Education and Sport Sciences, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran ,
Abstract: (1699 Views)
Background and objectives: The role of genetic components in expression of proteins involved in signaling pathways of fat and carbohydrate metabolism has been well-demonstrated. The aim of this study was to determine effects of high intensity interval training (HIIT) on glucose, insulin, and insulin resistance levels as well as IRS1 expression in gastrocnemius muscle of obese Wistar rats.
Methods: The study included 14 male, Wistar rats (aged 10 weeks) weighting 220 ± 20 g. Obesity was induced in all rats via exposure to a high-fat diet for six weeks. Then, the rats were randomly divided into a HIIT group (n=7) and a control group (n=7). The rats in the HIIT group performed treadmill running, five sessions a week, for eight weeks. Levels of fasting glucose, serum insulin, insulin resistance, and IRS1 expression in the gastrocnemius muscle of the rats were measured after the last training session. Data were analyzed by the independent t-test at statistical significance of 0.05.
Results: The HIIT intervention significantly decreased fasting glucose compared with the control group (p<0.0001). It also resulted in a significant decrease in serum insulin levels and insulin resistance compared with the control group (p<0.0001). Moreover, the HIIT training significantly increasedIRS 1 expression (p=0.030) in the gastrocnemius muscle of rats.
Conclusion: Based on the available evidence, the increase in insulin function and the decrease in insulin resistance can be attributed to increased IRS1 expression in the gastrocnemius muscle following HIIT training.
Methods: The study included 14 male, Wistar rats (aged 10 weeks) weighting 220 ± 20 g. Obesity was induced in all rats via exposure to a high-fat diet for six weeks. Then, the rats were randomly divided into a HIIT group (n=7) and a control group (n=7). The rats in the HIIT group performed treadmill running, five sessions a week, for eight weeks. Levels of fasting glucose, serum insulin, insulin resistance, and IRS1 expression in the gastrocnemius muscle of the rats were measured after the last training session. Data were analyzed by the independent t-test at statistical significance of 0.05.
Results: The HIIT intervention significantly decreased fasting glucose compared with the control group (p<0.0001). It also resulted in a significant decrease in serum insulin levels and insulin resistance compared with the control group (p<0.0001). Moreover, the HIIT training significantly increased
Conclusion: Based on the available evidence, the increase in insulin function and the decrease in insulin resistance can be attributed to increased IRS1 expression in the gastrocnemius muscle following HIIT training.
Research Article: Original Paper |
Subject:
Sport Physiology
Received: 2020/08/19 | Accepted: 2021/02/26 | Published: 2022/05/14 | ePublished: 2022/05/14
Received: 2020/08/19 | Accepted: 2021/02/26 | Published: 2022/05/14 | ePublished: 2022/05/14
References
1. Hersoug LG, Linneberg A. The link between the epidemics of obesity and allergic diseases: does obesity induce decreased immune tolerance? Allergy. 2007; 62(10): 1205-13. [View at Publisher] [DOI:10.1111/j.1398-9995.2007.01506.x] [PubMed] [Google Scholar]
2. Butler AE, Janson J, Bonner-Weir S, Ritzel R, Rizza RA, Butler PC. Beta-cell deficit and increased beta-cell apoptosis in humans with type 2 diabetes. Diabetes. 2003; 52: 102-110. [View at Publisher] [DOI:10.2337/diabetes.52.1.102] [PubMed]
3. Azizi F, Larijani B, Hossein panah F. Endicrine gland diseases. Endocrinology and Metabolism Research Center. 1ed. 1385.
4. Dowell P, Otto TC, Adi S, Lane MD. Convergence of peroxisome proliferator-activated receptor gamma and Foxo1 signaling pathways. J Biol Chem. 2003; 278(46): 45485-91. [View at Publisher] [DOI:10.1074/jbc.M309069200] [PubMed] [Google Scholar]
5. Tontonoz P, Spiegelman BM. Fat and beyond: The diverse biology of PPARgamma. Annu Rev Biochem. 2008; 77: 289-312. [View at Publisher] [DOI:10.1146/annurev.biochem.77.061307.091829] [PubMed] [Google Scholar]
6. Klöting N, Schleinitz D, Ruschke K, Berndt J, Fasshauer M, Tönjes A, et al. Inverse relationship between obesity and FTO gene expression in visceral adipose tissue in humans. Diabetologia. 2008; 51(4): 641-7. [View at Publisher] [DOI:10.1007/s00125-008-0928-9] [PubMed] [Google Scholar]
7. Almind K, Inoue G, Pederson O, Kahn CR. A common amino acid polymorphism in insulin receptor substrate-1 causes impaired insulin signaling. Evidence from transfection studies. J Clin Invest. 1996; 97: 2569-75. [View at Publisher] [DOI:10.1172/JCI118705] [PubMed] [Google Scholar]
8. White MF. The insulin signaling system and the IRS proteins. Diabetologia. 1997; 40: 2-17. [View at Publisher] [DOI:10.1007/s001250051387] [PubMed] [Google Scholar]
9. Jellema A, Zeegers MP, Feskens EJ, Dagnelie PC, Mensink RP. Gly972Arg variant in the insulin receptor substrate-1 gene and association with Type 2 diabetes: a meta-analysis of 27 studies. Diabetologia. 2003; 46: 990-5. [View at Publisher] [DOI:10.1007/s00125-003-1126-4] [PubMed] [Google Scholar]
10. Chibalin AV, Yu M, Ryder JW, Song XM, Galuska D, Krook A, et al. RExercise-induced changes in expression and activity of proteins involved in insulin signal transduction in skeletal muscle: differential effects on insulin-receptor substrates 1 and 2. Proc Natl Acad Sci USA. 2000; 97: 38-43. [View at Publisher] [DOI:10.1073/pnas.97.1.38] [PubMed] [Google Scholar]
11. Sharma N, Castorena CM, Cartee GD. Greater insulin sensitivity in calorie restricted rats occurs with unaltered circulating levels of several important myokines and cytokines. Nutr Metab (Lond). 2012; 9: 90-4. [View at Publisher] [DOI:10.1186/1743-7075-9-90] [PubMed] [Google Scholar]
12. Shaw K, Gennat H, O'Rourke P, Del Mar C. Exercise for Overweight or Obesity. Cochrane Database Syst Rev. 2006; 18(4): CD003817. [View at Publisher] [DOI:10.1002/14651858.CD003817.pub3] [PubMed] [Google Scholar]
13. Grygiel-Górniak B. Peroxisome proliferator-activated receptors and their ligands: nutritional and clinical implications--a review. Nutr J. 2014; 13: 17. [View at Publisher] [DOI:10.1186/1475-2891-13-17] [PubMed] [Google Scholar]
14. Kalhor H, Peeri M, Matin Homaee h, Izadi M. The Effect of 6 Weeks Resistance Training and HITT on GLP-1 Gene Expression of Diabetic Rats. Iranian Journal of Diabetes and Obesity. 2018; 10(1): 42-9. [View at Publisher] [Google Scholar]
15. Coughlin CC, Finck BN, Eagon JC, Halpin VJ, Magkos F, Mohammed BS, et al. Effect of marked weight loss on adiponectin gene expression and plasma concentrations. Obesity (Silver Spring) 2007; 15(3): 640-5. [View at Publisher] [DOI:10.1038/oby.2007.556] [PubMed] [Google Scholar]
16. Racil G, Ben Ounis O, Hammouda O, Kallel A, Zouhal H, Chamari K, Amri M. Effects of high vs. moderate exercise intensity during interval training on lipids and adiponectin levels in obese young females. Eur J Appl Physiol. 2013; 113(10): 2531-40. [View at Publisher] [DOI:10.1007/s00421-013-2689-5] [PubMed] [Google Scholar]
17. Tan S, Wang J, Cao L, Guo Z, Wang Y. Positive effect of exercise training at maximal fat oxidation intensity on body composition and lipid metabolism in overweight middle-aged women. Clin Physiol Funct Imaging. 2016; 36(3): 225-30. [View at Publisher] [DOI:10.1111/cpf.12217] [PubMed] [Google Scholar]
18. Abd El-Kader S, Gari A, Salah El-Den A. Impact of moderate versus mild aerobic exercise training on inflammatory cytokines in obese type 2 diabetic patients: a randomized clinical trial. Afr Health Sci. 2013; 13(4):857-63. [View at Publisher] [DOI:10.4314/ahs.v13i4.1] [Google Scholar]
19. Maltais ML, Perreault K, Courchesne-Loyer A, Lagacé JC, Barsalani R, Dionne IJ. Effect of Resistance Training and Various Sources of Protein Supplementation on Body Fat Mass and Metabolic Profile in Sarcopenic Overweight Older Adult Men: A Pilot Study. Int J Sport Nutr Exerc Metab. 2016; 26(1): 71-7. [View at Publisher] [DOI:10.1123/ijsnem.2015-0160] [PubMed] [Google Scholar]
20. Bouchonville M, Armamento-Villareal R, Shah K, Napoli N, Sinacore DR, Qualls C, Villareal DT. Weight loss, exercise or both and cardiometabolic risk factors in obese older adults: results of a randomized controlled trial. Int J Obes (Lond). 2013; 38: 423–431. [View at Publisher] [DOI:10.1038/ijo.2013.122] [Google Scholar]
21. Asarzadeh Noshabadi M, Abedi B. The effect of combained training on insulin resistance and some inflammation determinants in sedentary males. Quarterly of Ofoghe Danesh. 2012; 18(3): 96-101. [View at Publisher]
22. Ho TP, Zhao X, Courville AB, Linderman JD, Smith S, Sebring N, Della Valle DM, Fitzpatrick B, Simchowitz L, Celi FS. Effects of a 12-month moderate weight loss intervention on insulin sensitivity and inflammation status in nondiabetic overweight and obese subjects.Horm Metab Res. 2015;47(4):289-96. [DOI:10.1055/s-0034-1382011] [PubMed] [Google Scholar]
23. Samjoo IA, Safdar A, Hamadeh MJ, Raha S, Tarnopolsky MA.The effect of endurance exercise on both skeletal muscle and systemic oxidative stress in previously sedentary obese men.Nutr Diabetes. 2013; 3: 88. [View at Publisher] [DOI:10.1038/nutd.2013.30] [PubMed] [Google Scholar]
24. Steckling FM, Farinha JB, Santos DL, Bresciani G, Mortari JA, Stefanello ST, Courtes AA, Duarte T, Duarte MM, Moresco RN, Cardoso MS, Soares FA. High Intensity Interval Training Reduces the Levels of Serum Inflammatory Cytokine on Women with Metabolic Syndrome. Exp Clin Endocrinol Diabetes. 2016; 124(10): 597-601. [View at Publisher] [DOI:10.1055/s-0042-111044] [PubMed] [Google Scholar]
25. Kirwan JP, Del Aguila LF, Hernandez JM, Williamson DL, O'Gorman DJ, Lewis R, et al. Regular exercise enhances insulin activation of IRS-1-associated PI3-kinase in human skeletal muscle. Journal of Applied Physiology. 2000; 88(2): 797-803. [View at Publisher] [DOI:10.1152/jappl.2000.88.2.797] [PubMed] [Google Scholar]
26. Kim Y, Inoue T, Nakajima R, Nakae K, Tamura T, Tokuyama K, et al. Effects of endurance training ofgene expression on insulin signal transduction pathway. Biochemical and Biophysical Research Communications. 1995; 210(3): 766-73. [View at Publisher] [DOI:10.1006/bbrc.1995.1725] [PubMed] [Google Scholar]
27. Carvalho-Filho M, Ropelle E, Pauli R, Cintra D, Tsukumo D, Silveira L, et al. Expression of Concern: Aspirin attenuates insulin resistance in muscle of dietinduced obese rats by inhibiting inducible nitric oxide synthase production and S-nitrosylation of IRβ/IRS-1 and Akt. Diabetologia. 2017:1. [View at Publisher] [DOI:10.1007/s00125-017-4292-5] [PubMed] [Google Scholar]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Enamad
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.