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Volume 16, Issue 4 (Jul-Aug 2022)
mljgoums 2022, 16(4): 39-46 |
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Nouri A, Farzanegi P, Azarbayjani M A. Effects of Resveratrol Supplementation and Exercise on Apoptosis, Lipid Profile, and Expression of Farnesoid X Receptor, Liver X Receptor and Sirtuin 1 Genes in the Liver of Type 1 Diabetic Rats. mljgoums 2022; 16 (4) :39-46
URL: http://mlj.goums.ac.ir/article-1-1467-en.html
URL: http://mlj.goums.ac.ir/article-1-1467-en.html
1- Department of Exercise Physiology, Faculty of Humanities, Sari Branch, Islamic Azad University, Sari, Iran
2- Department of Exercise Physiology, Faculty of Humanities, Sari Branch, Islamic Azad University, Sari, Iran ,parvin.farzanegi@gmail.com
3- Department of Exercise Physiology, Islamic Azad University, Central Tehran Branch
2- Department of Exercise Physiology, Faculty of Humanities, Sari Branch, Islamic Azad University, Sari, Iran ,
3- Department of Exercise Physiology, Islamic Azad University, Central Tehran Branch
Abstract: (2148 Views)
Background and objectives: Diabetes mellitus is the most common metabolic disorder in the world. Here, we evaluated effects of resveratrol supplementation alone and combined with exercise on blood glucose, insulin, lipid profile, apoptosis biomarkers, and expression of farnesoid X receptor (Fxr), liver X receptor (Lxr), and sirtuin 1 (Sirt 1) genes in the liver of type 1 diabetic rats.
Methods: Streptozotocin was used to induce type 1 diabetes in Wistar rats. The rats were randomly assigned into seven groups. After treatment with resveratrol alone or combined with exercise training, the animals were sacrificed and lipid profile and levels of blood glucose and insulin were measured. Hepatocyte apoptosis was assessed by measuring the level of Bax and Bcl2 proteins using enzyme-linked immunosorbent assay kits. Expression of Fxr, Lxr, and Sirt1 was evaluated using real-time polymerase chain reaction. Comparison of the mean levels of all variables between different groups was performed using one-way analysis of variance, at statistical significance level of 0.05.
Results: Resveratrol significantly reduced the level of blood glucose and insulin compared with the control groups (p<0.001). It also significantly affected the lipid profile (p<0.001). Diabetes was significantly associated with decreased expression of Sirt1, Lxr, and Fxr and increased hepatocyte apoptosis. Resveratrol significantly improved the expression of all three genes (p<0.01). Overall, resveratrol supplementation combined with exercise was more effective than other methods.
Conclusion: The results indicate that that combination of resveratrol therapy with exercise could be beneficial for diabetic patients. However, more studies are needed to confirm this finding.
Methods: Streptozotocin was used to induce type 1 diabetes in Wistar rats. The rats were randomly assigned into seven groups. After treatment with resveratrol alone or combined with exercise training, the animals were sacrificed and lipid profile and levels of blood glucose and insulin were measured. Hepatocyte apoptosis was assessed by measuring the level of Bax and Bcl2 proteins using enzyme-linked immunosorbent assay kits. Expression of Fxr, Lxr, and Sirt1 was evaluated using real-time polymerase chain reaction. Comparison of the mean levels of all variables between different groups was performed using one-way analysis of variance, at statistical significance level of 0.05.
Results: Resveratrol significantly reduced the level of blood glucose and insulin compared with the control groups (p<0.001). It also significantly affected the lipid profile (p<0.001). Diabetes was significantly associated with decreased expression of Sirt1, Lxr, and Fxr and increased hepatocyte apoptosis. Resveratrol significantly improved the expression of all three genes (p<0.01). Overall, resveratrol supplementation combined with exercise was more effective than other methods.
Conclusion: The results indicate that that combination of resveratrol therapy with exercise could be beneficial for diabetic patients. However, more studies are needed to confirm this finding.
Keywords: Diabetes Mellitus [MeSH], Resveratrol [MeSH], Exercise [MeSH], Apoptosis [MeSH], Sirtuin 1 [MeSH], Liver X Receptors [MeSH]
Research Article: Original Paper |
Subject:
Sport Physiology
Received: 2021/12/15 | Accepted: 2022/02/12 | Published: 2022/07/16 | ePublished: 2022/07/16
Received: 2021/12/15 | Accepted: 2022/02/12 | Published: 2022/07/16 | ePublished: 2022/07/16
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