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1- Department of physical education and sports sciences, Aliabad katoul Branch, Islamic Azad University, Aliabad katoul, Iran
2- Department of physical education and sports sciences, Aliabad katoul Branch, Islamic Azad University, Aliabad katoul, Iran ,Dr.habibasgharpour@aliabadiau.ac.ir
3- Department of physical education and sports sciences, Sari Branch, Islamic Azad University, Sari, Iran
2- Department of physical education and sports sciences, Aliabad katoul Branch, Islamic Azad University, Aliabad katoul, Iran ,
3- Department of physical education and sports sciences, Sari Branch, Islamic Azad University, Sari, Iran
Abstract: (191 Views)
Aim: The aim of this study was to investigate the effect of swimming training, cell therapy and laser therapy on the expression of genes involved in mitochondrial dynamics in azoospermic rats.
Methods: In this experimental study, 30 rats 6 to 8 weeks of age were randomly selected, and then injected intraperitoneally with a 40 mg dose of busulfan for each rat and an azoospermia model was created. Then mice were subdivided into: patient, control, patient + laser, patient + exercise, patient + cell and patient + Cell + Laser + Exercise. Swimming training was performed for 8 weeks, 5 days a week and for 30 minutes every day.
Results: Induction of azoospermia significantly reduced the expression of Mfn2 in testicular tissue. The use of interventional methods increased the expression of Mfn2, which was significant only in the combined group laser + exercise. The induction of azoospermia also significantly increased the expression of Drp1 and Murf1 in testicular tissue. The use of exercise, cell therapy and laser therapy interventions reduced the expression of Drp1, which was not significant, but for Murf1 expression, testicular tissue decreased in all groups except for the cell group, it was significant.
Conclusion: Swimming training in combination with cell therapy and laser therapy by improving the expression of genes involved in mitochondrial dynamics may exert its protective effect in azoospermic mice and cause the mice to become fertile.
Methods: In this experimental study, 30 rats 6 to 8 weeks of age were randomly selected, and then injected intraperitoneally with a 40 mg dose of busulfan for each rat and an azoospermia model was created. Then mice were subdivided into: patient, control, patient + laser, patient + exercise, patient + cell and patient + Cell + Laser + Exercise. Swimming training was performed for 8 weeks, 5 days a week and for 30 minutes every day.
Results: Induction of azoospermia significantly reduced the expression of Mfn2 in testicular tissue. The use of interventional methods increased the expression of Mfn2, which was significant only in the combined group laser + exercise. The induction of azoospermia also significantly increased the expression of Drp1 and Murf1 in testicular tissue. The use of exercise, cell therapy and laser therapy interventions reduced the expression of Drp1, which was not significant, but for Murf1 expression, testicular tissue decreased in all groups except for the cell group, it was significant.
Conclusion: Swimming training in combination with cell therapy and laser therapy by improving the expression of genes involved in mitochondrial dynamics may exert its protective effect in azoospermic mice and cause the mice to become fertile.
Research Article: Research Article |
Subject:
Sport Physiology
Received: 2021/12/26 | Accepted: 2022/11/6
Received: 2021/12/26 | Accepted: 2022/11/6
References
1. Shapouri A, Asgharpour H, Farzanegi P, Aghaei Bahmanbeglo N. Regulation of increased expression of genes involved in mitochondrial dynamics of testicular tissue (PGC1-α and OPA1) in azoospermia model rats in interventions based on laser and physical activity. Jundishapur Scientific Medical Journal. 2024;23(1):89-102. [View at Publisher] [DOI] [Google Scholar]
2. Sun J, Brown TT, Samuels DC, Hulgan T, D'Souza G, Jamieson BD, et al. The role of mitochondrial DNA variation in age-related decline in gait speed among older men living with human immunodeficiency virus. Clinical Infectious Diseases. 2018; 67(5): 778-84. [View at Publisher] [DOI] [PMID] [Google Scholar]
3. Tang W-X, Wu W-H, Qiu H-Y, Bo H, Huang S-M. Amelioration of rhabdomyolysis-induced renal mitochondrial injury and apoptosis through suppression of Drp-1 translocation. Journal of nephrology. 2013; 26(6): 1073-82. [View at Publisher] [DOI] [PMID] [Google Scholar]
4. San-Millán I. The key role of mitochondrial function in health and disease. Antioxidants. 2023;12(4):782. [View at Publisher] [DOI] [PMID] [Google Scholar]
5. Zhang D, Liu X, Peng J, He D, Lin T, Zhu J, et al. Potential spermatogenesis recovery with bone marrow mesenchymal stem cells in an azoospermic rat model. International journal of molecular sciences. 2014; 15(8): 13151-65. [View at Publisher] [DOI] [PMID] [Google Scholar]
6. Mozafar A, Mehranani D, Vahdati A, Hosseini S, Forouzanfar M. The Effect of Bone Marrow Mesenchymal Stem Cell Culture in the Treatment of Azoospermic Infertility induced by Busulfan Balb/C mice. Armaghane Danesh. 2017; 22(3): 295-310. [View at Publisher] [Google Scholar]
7. Deihimi M, Azornia M, Takzare N. Effect of red and infrared spectrum low level of laser rays on Rat Seminiferous tubules. Journal of Gorgan University of Medical Sciences. 2010; 12(3): 10-7. [View at Publisher] [Google Scholar]
8. Zohrabi Karani L, Farzanegi P, Azarbayjani MA. The Effect of 8-Weeks of Low-Intensity Swimming Training on Promyelocytic Leukemia Zinc Finger Protein and Spermatid Transition Nuclear Protein Gene Expression in Azoospermic Rats Model. Internal Medicine Today. 2020; 26(4): 332-47. [View at Publisher] [DOI] [Google Scholar]
9. Abougalala FMA, Ali EK, Fayyad RMA, Elsaied MY, Abdelmonsef AS. Mesenchymal stem cells for Busulfan-Induced Azoospermia: An experimental study. International Journal of Medical Arts. 2022;4(4):2319-24. [View at Publisher] [DOI] [Google Scholar]
10. Abdelaal NE, Tanga BM, Abdelgawad M, Allam S, Fathi M, Saadeldin IM, et al. Cellular therapy via spermatogonial stem cells for treating impaired spermatogenesis, non-obstructive azoospermia. Cells. 2021;10(7):1779. [View at Publisher] [DOI] [PMID] [Google Scholar]
11. Cakici C, Buyrukcu B, Duruksu G, Haliloglu AH, Aksoy A, Isık A, et al. Recovery of fertility in azoospermia rats after injection of adipose‐tissue‐derived mesenchymal stem cells: the sperm generation. BioMed research international. 2013;2013(1):529589. [View at Publisher] [DOI] [PMID] [Google Scholar]
12. Morimoto H, Kanatsu-Shinohara M, Takashima S, Chuma S, Nakatsuji N, Takehashi M, et al. Phenotypic plasticity of mouse spermatogonial stem cells. PloS one. 2009; 4(11): e7909. [View at Publisher] [DOI] [PMID] [Google Scholar]
13. Memme JM, Erlich AT, Phukan G, Hood DA. Exercise and mitochondrial health. The Journal of physiology. 2021; 599(3): 803-17. [View at Publisher] [DOI] [PMID] [Google Scholar]
14. Zeng Z, Liang J, Wu L, Zhang H, Lv J, Chen N. Exercise-induced autophagy suppresses sarcopenia through Akt/mTOR and Akt/FoxO3a signal pathways and AMPK-mediated mitochondrial quality control. Frontiers in physiology. 2020;11:583478. [View at Publisher] [DOI] [PMID] [Google Scholar]
15. Bori Z, Zhao Z, Koltai E, Fatouros IG, Jamurtas AZ, Douroudos II, et al. The effects of aging, physical training, and a single bout of exercise on mitochondrial protein expression in human skeletal muscle. Experimental gerontology. 2012; 47(6): 417-24. [View at Publisher] [DOI] [PMID] [Google Scholar]
16. Fealy CE, Mulya A, Lai N, Kirwan JP. Exercise training decreases activation of the mitochondrial fission protein dynamin-related protein-1 in insulin-resistant human skeletal muscle. Journal of Applied Physiology. 2014; 117(3): 239-45. [View at Publisher] [DOI] [PMID] [Google Scholar]
17. Delroz H, Abdi A, Barari A, Farzanegi P. Protective Effect of aerobic training along with resveratrol on mitochondrial dynamics of cardiac myocytes in animal model of non-alcoholic fatty liver disease. Journal of Ardabil University of Medical Sciences. 2019; 19(3): 272-83. [View at Publisher] [DOI] [Google Scholar]
18. Fukumitsu K, Hatsukano T, Yoshimura A, Heuser J, Fujishima K, Kengaku M. Mitochondrial fission protein Drp1 regulates mitochondrial transport and dendritic arborization in cerebellar Purkinje cells. Molecular and Cellular Neuroscience. 2016; 71: 56-65. [View at Publisher] [DOI] [PMID] [Google Scholar]
19. Pang Z. Functions of the ubiquitin system in mammalian spermatogenesis and skeletal muscle. 2011. [View at Publisher] [Google Scholar]
20. Bodine SC, Baehr LM. Skeletal muscle atrophy and the E3 ubiquitin ligases MuRF1 and MAFbx/atrogin-1. American Journal of Physiology-Endocrinology and Metabolism. 2014; 307(6): E469-E84. [View at Publisher] [DOI] [PMID] [Google Scholar]
21. Cunha TF, Bacurau AV, Moreira JB, Paixão NA, Campos JC, et al. Exercise training prevents oxidative stress and ubiquitin-proteasome system overactivity and reverse skeletal muscle atrophy in heart failure. PLoS One. 2012; 7(8): e41701. [View at Publisher] [DOI] [PMID] [Google Scholar]
22. Chen G-Q, Mou C-Y, Yang Y-Q, Wang S, Zhao Z-W. Exercise training has beneficial anti-atrophy effects by inhibiting oxidative stress-induced MuRF1 upregulation in rats with diabetes. Life sciences. 2011; 89(1-2): 44-9. [View at Publisher] [DOI] [PMID] [Google Scholar]
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.