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Volume 18, Issue 3 (May-Jun 2024)
mljgoums 2024, 18(3): 21-24 |
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Koohsar F, Kalani H, Livani F, Jorjani O N, Yadagiri G, Ahmadi A et al . In vitro anti-leishmanial effects of garlic extract on Leishmania major: A Systematic Review. mljgoums 2024; 18 (3) :21-24
URL: http://mlj.goums.ac.ir/article-1-1600-en.html
URL: http://mlj.goums.ac.ir/article-1-1600-en.html
Faramarz Koohsar1 
, Hamed Kalani2 , Fatemeh Livani2 , Oghol Niaz Jorjani1 , Ganesh Yadagiri3 , Alireza Ahmadi1 , Roghiyeh Faridnia 4
, Hamed Kalani2 , Fatemeh Livani2 , Oghol Niaz Jorjani1 , Ganesh Yadagiri3 , Alireza Ahmadi1 , Roghiyeh Faridnia 4
1- Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran
2- Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran
3- Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, United States
4- Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran ,r_faridnia400@yahoo.com
2- Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran
3- Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, United States
4- Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran ,
Abstract: (393 Views)
Background: Leishmaniasis is a vector-borne parasitic disease caused by an obligate intracellular protozoan. Despite the significant frequency of cutaneous leishmaniasis, there is still no appropriate prevention, control and treatment. In recent years, a few studies have been performed on the effects of garlic extract for the treatment of cutaneous leishmaniasis. Current drugs, such as glucantime, have several side effects and lead to direct cellular damage. If an herbal-based drug reduces Leishmania by augmenting the immune system, it has the potential to possess a wider margin of safety. This study aimed to investigate the effects of in vitro systematic review of the effects of garlic (Allium sativum) and its compounds on Leishmania major.
Methods: Five English databases (PubMed, Google Scholar, Embase, Scopus, and Web of Science) were searched until the end of December 2022. The syntax and specific tags have been used for each database. The studies with poor methodology, inadequate information, inappropriate analysis, and confusing presentation were excluded from the current study. The quality of articles was assessed by eleven questions developed by the authors and the data were extracted from the selected articles and discussed.
Results: In this study, 198 articles were selected in the search step, of which five eligible articles were included for examination. The most commonly used solvent for the preparation of garlic extract was distilled water (60%), followed by methanol (40%). In these studies, the bulb of garlic is mostly used (80%). Cell type of J774 was used in only one study (20%), and murine peritoneal macrophages were used in the other studies (80%). The effect of garlic on Leishmania major was strong (80%). Studies have shown that garlic extract or its active compounds can increase cellular immune responses, which play an important role in inhibiting the Leishmania parasite. This is associated with the activation of macrophages and increased IFN-γ levels and NO production.
Conclusion: According to the studies, the effect of different combinations of garlic on the Leishmania parasite has been shown. However, the exact mechanism of the anti-Leishmanial effect of garlic has not been determined. Thus, this issue needs further investigation.
Methods: Five English databases (PubMed, Google Scholar, Embase, Scopus, and Web of Science) were searched until the end of December 2022. The syntax and specific tags have been used for each database. The studies with poor methodology, inadequate information, inappropriate analysis, and confusing presentation were excluded from the current study. The quality of articles was assessed by eleven questions developed by the authors and the data were extracted from the selected articles and discussed.
Results: In this study, 198 articles were selected in the search step, of which five eligible articles were included for examination. The most commonly used solvent for the preparation of garlic extract was distilled water (60%), followed by methanol (40%). In these studies, the bulb of garlic is mostly used (80%). Cell type of J774 was used in only one study (20%), and murine peritoneal macrophages were used in the other studies (80%). The effect of garlic on Leishmania major was strong (80%). Studies have shown that garlic extract or its active compounds can increase cellular immune responses, which play an important role in inhibiting the Leishmania parasite. This is associated with the activation of macrophages and increased IFN-γ levels and NO production.
Conclusion: According to the studies, the effect of different combinations of garlic on the Leishmania parasite has been shown. However, the exact mechanism of the anti-Leishmanial effect of garlic has not been determined. Thus, this issue needs further investigation.
Research Article: Systematic Review |
Subject:
Parasitology
Received: 2022/12/5 | Accepted: 2023/07/31 | Published: 2024/05/10 | ePublished: 2024/05/10
Received: 2022/12/5 | Accepted: 2023/07/31 | Published: 2024/05/10 | ePublished: 2024/05/10
References
1. Vandeputte M, Henten S, Griensven J, Huits R, Esbroeck M, Huits R, Esbroeck M, Auwera GV, et al. Epidemiology, clinical pattern and impact of species-specific molecular diagnosis on management of leishmaniasis in Belgium, 2010-2018: A retrospective study. Travel Med Infect Dis
. 2020;38:101885. [View at Publisher] [DOI] [PMID] [Google Scholar]
2. Faridnia R, Kalani H, Ziaei Hezarjaribi H, Denny P, Rafie A, Fakhar M, et al. Apoptotic blebs from Leishmania major-infected macrophages as a new approach for cutaneous leishmaniasis vaccination. Microb Pathog. 2020;147:104406. [View at Publisher] [DOI] [PMID] [Google Scholar]
3. Hernández-Bojorge S, Blass-Alfaro G, Rickloff M, Gómez-Guerrero M, Izurieta R. Epidemiology of cutaneous and mucocutaneous leishmaniasis in Nicaragua. Parasite Epidemiol Control. 2020;11:e00192.. [View at Publisher] [DOI] [PMID] [Google Scholar]
4. Akhtari J, Faridnia R, Kalani H, Bastani R, Fakhar M, Rezvan H, et al. Potent in vitro antileishmanial activity of a nanoformulation of cisplatin with carbon nanotubes against Leishmania major. J Glob Antimicrob Resist. 2019;16:11-6. [View at Publisher] [DOI] [PMID] [Google Scholar]
5. Bahmani M, Saki K, Ezatpour B, Shahsavari A, Eftekhari A, Jelodari M, et al. Leishmaniosis phytotherapy: Review of plants used in Iranian traditional medicine on leishmaniasis. Asian Pac J Trop Biomed. 2015;5(9):695-701. [View at Publisher] [DOI] [Google Scholar]
6. Iciek M, Kwiecien I, Wlodek L. Biological properties of garlic and garlic-derived organosulfur compounds. Environ Mol Mutagen. 2009;50(3):247-65. [View at Publisher] [DOI] [PMID] [Google Scholar]
7. Anthony JP, Fyfe L, Smith H. Plant active components-a resource for antiparasitic agents. Trends Parasitol. 2005;21(10):462-8. [View at Publisher] [DOI] [PMID] [Google Scholar]
8. Gharavi M, Nobakht M, Khademvatan SH, Bandani E, Bakhshayesh M, Roozbehani M. The Effect of Garlic Extract on Expression of INFγ and Inos Genes in Macrophages Infected with Leishmania major. Iran J Parasitol. 2011;6(3):74-81. [View at Publisher] [PMID] [Google Scholar]
9. Knobloch K, Yoon U, Vogt PM. Preferred reporting items for systematic reviews and metaanalyses (PRISMA) statement and publication bias. J Craniomaxillofac Surg. 2011;39(2):91-2. [View at Publisher] [DOI] [PMID] [Google Scholar]
10. Ahmadi-Renani K, Mahmoodzadeh A, Cheraghali A, Esfahani A. Effect of garlic extract on cutaneous leishmaniasis and the role of nitric oxide. Iran J Med Sci. 2002;27(3):97-100. [View at Publisher] [Google Scholar]
11. Kinuthia GK, Kabiru EW, Anjili CO, Kigondu EM, Ngure VN, Ingonga JM, et al. Efficacy of crude methanolic extracts of Allium sativum L. and Moringa stenopetala (Baker f.) Cufod. against Leishmania major. Int J Med Arom Plants. 2014;4(1):16-25. [View at Publisher] [Google Scholar]
12. Gharavi M, Nobakht M, Khademvatan S, Fani F, Bakhshayesh M, Roozbehani M. The effect of aqueous garlic extract on interleukin-12 and 10 levels in Leishmania major (MRHO/IR/75/ER) infected macrophages. Iran J Public Health. 2011;40(4):105-11. [View at Publisher] [PMID] [Google Scholar]
13. Wabwoba BW, Anjili CO, Ngeiywa MM, Ngure PK, Kigondu EM, Ingonga J, et al. Experimental chemotherapy with Allium sativum (Liliaceae) methanolic extract in rodents infected with Leishmania major and Leishmania donovani. J Vector Borne Dis. 2010;47(3):160-7. [View at Publisher] [PMID] [Google Scholar]
14. Ghazanfari T, Hassan ZM, Khamesipour A. Enhancement of peritoneal macrophage phagocytic activity against Leishmania major by garlic (Allium sativum) treatment. J Ethnopharmacol. 2006;103(3):333-7. [View at Publisher] [DOI] [PMID] [Google Scholar]
15. Singh R, Singh K. Garlic: A spice with wide medicinal actions. J pharmacogn Phytochem. 2019;8(1):1349-55. [View at Publisher] [Google Scholar]
16. Avato P, Tursi F, Vitali C, Miccolis V, Candido V. Allylsulfide constituents of garlic volatile oil as antimicrobial agents. Phytomedicine. 2000;7(3):239-43. [View at Publisher] [DOI] [PMID] [Google Scholar]
17. Foroutan-Rad M, Hazrati Tappeh K, Khademvatan Sh. Antileishmanial and Immunomodulatory Activity of Allium sativum (Garlic): A Review. J Evid Based Complementary Altern Med. 2017;22(1):141-55. [View at Publisher] [DOI] [PMID] [Google Scholar]
18. Gamboa‐León M, Aranda‐González I, Mut‐Martín M, García‐Miss M, Dumonteil E. In vivo and In vitro Control of Leishmania mexicana due to Garlic‐induced NO Production. Scand J Immunol. 2007;66(5):508-14. [View at Publisher] [DOI] [PMID] [Google Scholar]
19. Gamboa-Leon R, Vera-Ku M, Peraza-Sanchez SR, Ku-Chulim C, Horta-Baas A, Rosado-Vallado M. Antileishmanial activity of a mixture of Tridax procumbens and Allium sativum in mice. Parasite. 2014;21:15. [View at Publisher] [DOI] [PMID] [Google Scholar]
20. Khademvatan S, Saki J, Gharavi MJ, Rahim F. Allium sativum extract induces apoptosis in Leishmania major (MRHO/IR/75/ER) promastigotes. J Med Plant Res. 2011;5(16):3725-32. [View at Publisher] [Google Scholar]
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