Fri, Jul 25, 2025
Parisa Hasanein1 
, Fahime Javadi Hedaiat Abad2 , Mousa Bohlooli3 , Mostafa Khajeh4 , Sedigheh Esmaielzadeh Bahabadi2 , Neda Poormolaei2
, Fahime Javadi Hedaiat Abad2 , Mousa Bohlooli3 , Mostafa Khajeh4 , Sedigheh Esmaielzadeh Bahabadi2 , Neda Poormolaei2
1- Department of Biology, School of Basic Sciences, University of Zabol, Zabol, Iran , parisa.hasanein@gmail.com
2- Department of Biology, School of Basic Sciences, University of Zabol, Zabol, Iran
3- Department of Biology, School of Basic Sciences, University of Zabol, Zabol, Iran; Department of Cell and Molecular Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
4- Department of Chemistry, School of Basic Sciences, University of Zabol, Zabol, Iran
2- Department of Biology, School of Basic Sciences, University of Zabol, Zabol, Iran
3- Department of Biology, School of Basic Sciences, University of Zabol, Zabol, Iran; Department of Cell and Molecular Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
4- Department of Chemistry, School of Basic Sciences, University of Zabol, Zabol, Iran
Abstract: (1095 Views)
Background: DNA glycation, a process where Glc non-enzymatically binds to DNA, is implicated in various detrimental effects, including strand breaks, mutations, and altered gene expression. This damage is considered a significant contributor to the pathogenesis of diabetes mellitus and its associated complications. Consequently, there has been increasing interest in identifying antiglycation agents as a strategy for preventing and mitigating these complications. Prior research has indicated that extracts from Tamarix aphylla (T. aphylla) leaves possess antidiabetic properties. Therefore, this study aimed to investigate, for the first time, the impact of T. aphylla extract on Glc-mediated DNA glycation.
Methods: DNA samples were incubated with Glc over a four-week period. Subsequently, the modulatory effects of T. aphylla on Glc-induced DNA structural alterations were investigated employing a range of analytical techniques. These methodologies encompassed ultraviolet-visible (UV-Vis) spectroscopy, fluorescence spectroscopy, circular dichroism (CD) spectroscopy, and agarose gel electrophoresis.
Results: The results obtained from UV–Vis and fluorescence spectroscopy demonstrated that T. aphylla extract led to a reduction in the formation of DNA-advanced glycation end products (AGEs). Furthermore, CD spectroscopy and agarose gel electrophoresis analyses indicated that the structural alterations of glycated DNA were diminished in the presence of T. aphylla extract.
Conclusion: Based on the evidence presented, T. aphylla demonstrates protective properties against DNA glycation. Consequently, pending further rigorous investigation, it may represent a potentially valuable therapeutic agent for mitigating the detrimental consequences of glycation, particularly in environments characterized by elevated Glc concentrations and hyperglycemic states.
Methods: DNA samples were incubated with Glc over a four-week period. Subsequently, the modulatory effects of T. aphylla on Glc-induced DNA structural alterations were investigated employing a range of analytical techniques. These methodologies encompassed ultraviolet-visible (UV-Vis) spectroscopy, fluorescence spectroscopy, circular dichroism (CD) spectroscopy, and agarose gel electrophoresis.
Results: The results obtained from UV–Vis and fluorescence spectroscopy demonstrated that T. aphylla extract led to a reduction in the formation of DNA-advanced glycation end products (AGEs). Furthermore, CD spectroscopy and agarose gel electrophoresis analyses indicated that the structural alterations of glycated DNA were diminished in the presence of T. aphylla extract.
Conclusion: Based on the evidence presented, T. aphylla demonstrates protective properties against DNA glycation. Consequently, pending further rigorous investigation, it may represent a potentially valuable therapeutic agent for mitigating the detrimental consequences of glycation, particularly in environments characterized by elevated Glc concentrations and hyperglycemic states.
Research Article: Research Article |
Subject:
Biochemistry
Received: 2024/08/30 | Accepted: 2024/12/31
Received: 2024/08/30 | Accepted: 2024/12/31
References
1. Voziyan PA, Khalifah RG, Thibaudeau C, Yildiz A, Jacob J, Serianni AS, et al. Modification of proteins in vitro by physiological levels of glucose: pyridoxamine inhibits conversion of Amadori intermediate to advanced glycation end-products through binding of redox metal ions. J Biol Chem. 2003; 27: 46616-46624. [View at Publisher] [DOI] [PMID] [Google Scholar]
2. Stitt AW. Advanced glycation: an important pathological event in diabetic and age related ocular disease. Br J Ophthalmol. 2001; 85: 746-753. [View at Publisher] [DOI] [PMID] [Google Scholar]
3. Münch G, Shepherd CE, McCann H, Brooks WS, Kwok JB, Arendt T, et al. Intraneuronal advanced glycation end products in presenilin-l Alzheimer's disease. Neuroreport 2002; 13: 601-604. [View at Publisher] [DOI] [PMID] [Google Scholar]
4. Thilavech T, Marnpae M, Mäkynen K, Adisakwattana S. Phytochemical composition, antiglycation, antioxidant activity and methylglyoxal-trapping action of brassica vegetables. Plant Foods Hum Nutr. 2021; 76; 340-346. [View at Publisher] [DOI] [PMID] [Google Scholar]
6. Qadir MI, Abbas K, Hamayun R, Ali M. Analgesic, anti-inflammatory and anti-pyretic activities of aqueous ethanolic extract of Tamarix aphylla L. (Saltcedar) in mice. Pak J Pharm Sci. 2014; 27(6): 1985-8. [View at Publisher] [PMID] [Google Scholar]
7. Alshehri SA, Wahab S, Abullais SS, Das G, Hani U, Ahmad W, et al. Pharmacological Efficacy of Tamarix aphylla: A Comprehensive Review. Plants (Basel). 2021; 11(1): 118. [View at Publisher] [DOI] [PMID] [Google Scholar]
8. Gadallah AS, Mujeeb-Ur-Rehman, Atta-Ur-Rahman, Yousuf S, Atia-Tul-Wahab, Jabeen A, et al. Anti-Inflammatory Principles from Tamarix aphylla L.: A Bioassay-Guided Fractionation Study. Molecules. 2020; 25(13): 2994. [View at Publisher] [DOI] [PMID] [Google Scholar]
9. Mahfoudhi A, Salem AB, Garrab M, Hammami S, Gorcii M, Mastouri M, et al. Antioxidant and antimicrobial activities of Tamarix aphylla (L.) Karst. growing in Tunisia. Mor J Chem. 2016; 4(4): 987-995. [View at Publisher] [Google Scholar]
10. Qnais E, Alqudah A, Wedyan M, Athamneh RY, Bseiso Y, Abudalo R, et al. Potential anti-inflammatory activity of the Tamarix aphylla essential. Pharmacia. 2023; 70(3): 707-771. [View at Publisher] [DOI] [Google Scholar]
11. Ahmed Tariq RU, Khan N, Sharif N, Din ZU, Mansoor K. Antihyperglycemic effect of methanol extract of Tamarix aphylla L. Karst (Saltcedar) in streptozocin-nicotinamide induced diabetic rats. Asian Pacific J Tropic Biomed. 2017; 7(6): 619-623. [View at Publisher] [DOI] [Google Scholar]
12. Ashraf JM, Arif B, Dixit K, Alam K. Physicochemical analysis of structural changes in DNA modified with glucose. Int J Biol Macromol. 2012; 51: 604-611. [View at Publisher] [DOI] [PMID] [Google Scholar]
13. Bagherzadeh-Yazdi M, Bohlooli M, Khajeh M, Ghamari F, Ghaffari-Moghaddam M, Poormolaie N, et al. Acetoacetate enhancement of glucose mediated DNA glycation. Biochem Biophys Rep. 2020; 25: 100878. [View at Publisher] [DOI] [PMID] [Google Scholar]
14. Bohlooli M, Miri M, Khajeh M, et al. Inhibitory influence of 3-β-hydroxybutyrate on calf thymus DNA glycation by glucose. RSC Advances. 2016; 87: 83880-83884. [View at Publisher] [DOI] [Google Scholar]
15. Chobot A, Górowska-Kowolik K, Sokołowska M, Jarosz-Chobot P. Obesity and diabetes-Not only a simple link between two epidemics. Diabetes Metab Res Rev. 2018; 34(7): e3042. [View at Publisher] [DOI] [PMID] [Google Scholar]
16. Ali A, More TA, Hoonjan AK, Sivakami S. Antiglycating Potential of Acesulfame Potassium: An Artificial Sweetener. Appl Physiol Nutr Metab. 2017; 42: 1054-1063. [View at Publisher] [DOI] [PMID] [Google Scholar]
18. Mustafa I, Ahmad S, Dixit K, Moinuddin, Ahmad J, Ali A. Glycated human DNA is a preferred antigen for anti-DNA antibodies in diabetic patients. Diabetes Res Clin Pract. 2012; 95: 98-104. [View at Publisher] [DOI] [PMID] [Google Scholar]
19. Al-Otibi F, Moria GA, Alharbi RI, Yassin MT, Abdulaziz A, Al-Askar A. The antifungal properties of tamarix aphylla extract against some plant pathogenic fungi. Microorganisms. 2023; 11(1): 127. [View at Publisher] [DOI] [PMID] [Google Scholar]
21. Booth AA, Khalifah RG, Hudson BG. Thiamine pyrophosphate and pyridoxamine inhibit the formation of antigenic advanced glycation end-products: comparison with aminoguanidine, Biochem Biophys Res Commun. 1996; 220: 113-119. [View at Publisher] [DOI] [PMID] [Google Scholar]
22. Brownlee M, Vlassara H, Kooney A, Ulrich P, Cerami A. Aminoguanidine prevents diabetes-induced arterial wall protein cross-linking. Science. 1986; 232: 1629-1632. [View at Publisher] [DOI] [PMID] [Google Scholar]
23. Morimitsu Y, Yoshida K, Esaki S, Hirota A. Protein glycation inhibitors from thyme (Thymus vulgaris). Biosci Biotechnol Biochem 1995; 59: 2018-2021. [View at Publisher] [DOI] [PMID] [Google Scholar]
24. Urios P, Grigorova-Borsos AM, Sternberg M. Aspirin inhibits the formation of pentosidine, a cross-linking advanced glycation end product, in collagen, Diabetes Res Clin Pract. 2007; 77: 337-340. [View at Publisher] [DOI] [PMID] [Google Scholar]
25. Balyan P, Shamsul Ola M, Alhomida AS, Ali A. D-ribose-induced glycation and its attenuation by the aqueous extract of nigella sativa seeds. Medicina (Kaunas). 2022; 58(12): 1816. [View at Publisher] [DOI] [PMID] [Google Scholar]
26. Ahmad S, Dixit K, Shahab U, Alam K, Ali A. Genotoxicity and immunogenicity of DNA-advanced glycation end products formed by methylglyoxal and lysine in presence of Cu2. Biochem. Biophys. Res. Commun. 2011; 407: 568-574. [View at Publisher] [DOI] [PMID] [Google Scholar]
27. Thornalley PJ. Monosaccharide autoxidation in health and disease. Environ. Health Perspect. 1985; 64: 297-307. [View at Publisher] [DOI] [PMID] [Google Scholar]
28. Ahmad S, Shahab U, Baig MH, Khan MS, Khan MS, Srivastava AK, et al. Inhibitory effect of metformin and pyridoxamine in the formation of early, intermediate and advanced glycation end-products. PloS One. 2013; 8; e72128. [View at Publisher] [DOI] [PMID] [Google Scholar]
30. Ahmad S, Moinuddin, Shahab U, Habib S, Salman Khan M, Alam K, Ali A. Glycoxidative damage to human DNA: neo-antigenic epitopes on DNA molecule could be a possible reason for autoimmune response in type 1 diabetes. Glycobiol. 2014; 24: 281-291. [View at Publisher] [DOI] [PMID] [Google Scholar]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Enamad
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.