en ميکروب شناسي Microbiology Research Article Research Article <span style="line-height:normal"><span style="tab-stops:517.5pt 7.25in"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Abstract </span></span></b></span></span><br> <span style="line-height:normal"><span style="tab-stops:517.5pt 7.25in"><b><span style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"></span></span></b></span></span><br> <span style="line-height:normal"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Background and Objective:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> Currently, healthcare-associated infections (HAIs) are rising alarmingly due to antibiotic-resistant gram-negative bacteria, particularly <i>Pseudomonas aeruginosa</i> and <i>Acinetobacter</i> species. The study in Rasht focuses on identifying phenotypic and molecular metallo-&beta;-lactamases (MBLs) in clinical isolates of <i>Pseudomonas aeruginosa</i> and <i>Acinetobacter</i> that display resistance to Imipenem.</span></span></span><br> <span style="line-height:normal"><span style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span><br> <span style="line-height:normal"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Methods: </span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">In this study, 52 <i>Acinetobacter </i>and 25 <i>Pseudomonas aeruginosa</i> samples were collected from medical centers in Rasht. The clinical isolates were analyzed using various biochemical tests. The antibiotic resistance patterns of the isolates were determined by the disk diffusion method, following CLSI guidelines. Isolates producing metallo-&beta;-lactamases (MBLs) were identified using the IPM+EDTA combined test method. Genomic DNA was subsequently extracted from the isolates using a commercial kit, and the presence of <i>bla</i>_IMP and <i>bla</i>_VIM genes was determined by PCR.</span></span></span><br> <span style="line-height:normal"><b><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"></span></span></b></span><br> <span style="line-height:normal"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Results:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> According to the findings of this study, the highest percentage of resistance in Acinetobacter was observed with cefotaxime antibiotics at 94.23%, and in <i>Pseudomonas aeruginosa </i>with ceftazidime at 84%. Among the clinical isolates not susceptible to Imipenem, 18 (40%) were <i>Acinetobacter</i> and 2 (15.38%) were <i>Pseudomonas aeruginosa</i>, as determined by the combined disc method. The <i>bla</i>_IMP and <i>bla</i>_VIM genes were detected in 7 (13.46%) and 11 (21.15%) of the <i>Acinetobacter</i> samples, respectively, and in 8 (32%) and 5 (20%) of the<i> Pseudomonas aeruginosa</i> samples, respectively.</span></span></span><br> <span style="line-height:normal"><span style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span><br> <b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""> These results suggest that the <i>bla</i>_IMP and <i>bla</i>_VIM genes play important roles in the antibiotic resistance and potentially influence the virulence of <i>Acinetobacter</i> species and <i>Pseudomonas aeruginosa</i> in the region</span></span></span> Acinetobacter, Pseudomonas aeruginosa, blaIMP, blaVIM, Metallo beta lactamases 0 0 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1787-1&slc_lang=en&sid=1 Zahra Nasirzadeh zahranasirzade94@gmail.com 100319475328460032155 100319475328460032155 Yes Department of Biology, Ra.C., Islamic Azad University, Rasht, Iran Seyedeh Tooba Shafighi shafighi@iau.ac.ir 100319475328460032156 100319475328460032156 No Department of Biology, Ra.C., Islamic Azad University, Rasht, Iran