en ایمنی شناسی Immunology گزارش مورد Case Report <span style="font-size:11pt"><span style="line-height:150%"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Anti-centromere antibodies (ACA) are most commonly identified by indirect immunofluorescence (IIF) on HEp-2 cells, where they usually produce the characteristic centromere pattern (AC-3) associated with limited cutaneous systemic sclerosis. With the increasing routine use of solid-phase assays such as ELISA, situations arise in which assay results do not fully align. We report the case of a 75-year-old woman with features suggestive of systemic autoimmune disease whose ANA testing by IIF demonstrated a multiple nuclear dots pattern (AC-6) with elements resembling a CENP-F&ndash;like pattern (AC-14), rather than the classic centromere pattern. In contrast, solid-phase testing detected anti-centromere reactivity. This case illustrates how pattern recognition on IIF and antigen-specific assays may diverge and underscores the importance of interpreting serologic results within clinical context and across complementary laboratory platforms.</span></span></span><b><span style="font-size:12.0pt"><span style="line-height:150%"><span style="font-family:"Times New Roman",serif"></span></span></span></b></span></span></span></span><br> &nbsp; Anti-centromere antibodies (ACA), Indirect immunofluorescence (IIF), ELISA, ANA patterns (AC-3, AC-6, AC-14), CENP-F–like pattern 0 0 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1140-3&slc_lang=en&sid=1 Mohammadreza Sheikh Sajjadieh mohammad_esfahan@yahoo.com 100319475328460032437 100319475328460032437 Yes Member of AIMS Australia, and, Consultant Clinical Immunology, Padtan Danesh Company, Tehran, Iran Olena Sheikh Sajjadieh sheikhsajjdieh@gmail.com 100319475328460032438 0009-0000-9624-244X No MBBS, General Practitioner, Australia Ghazaleh Aghababaei aghababaeighaleh@gmail.com 100319475328460032439 100319475328460032439 No Technical Officer, Padtan Danesh Company, Tehran, Iran Mohammad Farzi farzi@ptdlab.com 100319475328460032440 100319475328460032440 No CEO, Padtan Danesh Company, Tehran, Iran Abdolsamad Ghorbany info@vahid-lab.com 100319475328460032441 100319475328460032441 No Doctorate of laboratory medicine, Vahid Laboratory Director, Isfahan, Iran