This paper should be cited as:
Amoozgari, Z. Salehi pour bavarsad, S. Noorbehbahani, M.
Effect of α-lipoic
Acid on Hemolytic Activity of Iranian Vipera Lebetina Venom
Amoozgari, Z. (MSc)1, Salehi
pour bavarsad, S. (BSc)2, Noorbehbahani,
M. (BSc)3
1. MSc of Biochemistry, Faculty of Medicine, Ahvaz
jundishapure University of Medical Sciences, Ahvaz,
Iran
2. MSc Student of Biochemistry, Faculty of
Medicine, Ahvaz jundishapure University of Medical
Sciences, Ahvaz, Iran
3. BSc of medical Science, Faculty of Medicine,
Ahvaz jundishapure University of Medical Sciences,
Ahvaz, Iran
Abstract
Background and Objective: Snake venom is a complex of several toxic elements and enzymes. It has the agents with the ability to destroy
cellular and subcellular membrane and to bring about hemolysis of red blood
cells (RBC). Two types of direct and indirect hemolytic activity are known in
snake venom in that phospholipase A2 is responsible for the indirect lysis. The aim of this study was to investigate the effect
of α-lipoic acid on hemolytic activity of
Iranian Vipera Lebetina
venom.
Material and Methods: Protein concentration of the crude venom of Vipera
Lebetina was determined using bovine serum albumin as
a standard. Direct hemolytic activity of venom was determined by using the
Human RBC and Indirect hemolytic activity was assayed on RBC in the presence of
egg yolk. Then, α-lipoic acid with different
concentrations in 100 mM Tris-HCL
buffer was applied and its effect on hemolysis of RBC was studied.
Results: direct hemolytic activity on RBC was not observed while its indirect
activity was detected to be increased proportional to different concentration
of α-lipoic acid. The range of indirect hemolysis was increased
up to 60% by 60µm α-lipoic acid.
Conclusion: Not only has α-lipoic acid no inhibitory
effects on the hemolytic activity of Iranian Vipera Lebetina venom but also has the positive effects on it.
Keywords: Iranian Vipera Lebetina
Venom, Direct Hemolytic, Indirect Hemolytic, α-Lipoic
Acid, Phospholipase A2
Corresponding
Author: Amoozgari, Z.
Email: Zamoozgari277@yahoo.com
Received: 11
May 2014
Revised: 4 Jul 2014
Accepted: 8 Jul 2014
This paper should be cited
as: Amoozgari Z, Salehi
pour bavarsad S, Noorbehbahani
M. [Effect of α-lipoic Acid on Hemolytic
Activity of Iranian Vipera Lebetina
Venom]. mljgoums. 2015; 9(1):
70-75[Article in Persian]