This paper should be cited as: Najar Peerayeh, SH. Derakhshan, S. Fallah, F. Bakhshi, B.

Antimicrobial Resistance Pattern and Prevalence of Class 1, 2, and 3 Integrons in Clinical Isolates of Klebsiella Pneumoniae in Loghman-E Hakim Hospital, Tehran

Najar Peerayeh, SH. (PhD)¹, Derakhshan, S. (PhD)², Fallah, F. (PhD)³, Bakhshi, B. (PhD)⁴

1. Associate professor of Medical Bacteriology, Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

2. Assistant professor of Medical Bacteriology, Department of Microbiology, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran

3. Professor of Medical Microbiology, Pediatric Infections Research Center, Tehran, Iran

4. Assistant professor of Medical Microbiology, Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Abstract

Background and Objective:Multiple drug resistance has increased in recent years in Klebsiella pneumoniae isolates.The Integrons are mobile genetic elements that carry antibiotics resistance genes. The aim of this study was to determine antibiotic susceptibility and the prevalence of class 1, 2, and 3 integrons in clinical Klebsiella pneumoniae isolated from clinical specimens.

Material and Methods:A total of 108 K. pneumoniae isolates were collected between April and December 2011 from different clinical specimens of Loghman hospital in Tehran and identified by biochemical tests. Susceptibility of isolates to 14 antibiotic disks was determined by disk diffusion method.The template DNA was extracted by freeze-thaw method and the presence of class1,2, and 3 integrons was investigated by PCR method. Level of resistance to antibiotics in integron-positive and integron-negative isolates was determined.

Results:The highest level of resistance was seen for cefotaxime,ceftriaxone, and amoxicillin-clavulanic acid (55.5%). In 79 isolates (73.14%) class 1 integron and  in 57 of 79 isolates (72.15%)resistance to at least two classes of drugs were seen. The class 2 and 3 integrons were not detected. Among integron-negative isolates, 8 isolates (27.58%) had resistance to at least one antibiotic.

Conclusion:The prevalence of class 1 integron in resistant K. pneumoniae is high;therefore, the monitoring of drug resistance and limiting the use of antibiotics are necessary.

Keywords: Klebsiella Pneumoniae, Integron, Multi-Drug Resistance

Corresponding Author:Najar Peerayeh, SH.

Email: najarp_s@modares.ac.ir

Received: 6 Oct 2013
Revised: 6 Jun 2013
Accepted: 1 Jul 2013