This paper should be cited as:Hajihasani,A. Douraghi,M. Rahbar, M. Mohammadzadeh,M. Zeraati, H.Ghoorchian, S.Alavi Moghadam,M. Sabzi,M.

Hajihasani, A. (MSc)1, Douraghi, M. (PhD)2, Rahbar, M. (PhD)3 Mohammadzadeh, M. (MSc)4 Zeraati, H. (PhD)5, Ghoorchian, S. (BSc)6 Alavi Moghadam, M. (PhD)7 Sabzi, M. (BSc)8

1. MSc of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

2. Assistant Professor of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

3. Professor of Microbiology, Department of Microbiology, Milad Hospital, Tehran, Iran

4. MSc of Microbiology, Department of Microbiology, Milad Hospital, Tehran, Iran

5. Associate Professor of Biostatistic, Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

6. BSc of Laboratory Sciences , Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

7. Associate Professor of Emergency Medicine, Department of Infection Control, Imam Hossein General Hospital, Tehran, Iran

8. BSc of Nursing, Department of Infection Control, Imam Hossein Hospital, Tehran, Iran

Isolation and Identification of Stenotrphomonas Maltophilia from the Hospitals of Tehran City

Abstract

Background and Objective:Stenotrphomonas maltophilia is an opportunistic nosocomial pathogen with high mortality in immunocompromised cases.The aim of this study was to isolate and identify Stenotrphomonas maltophilia in the hospitals’ environment and wards.

Material and Methods:In this cross-sectional study, a total of 1108 samples were collected from environment of two hospitals during 12 months. Identification of isolates was performed using biochemical, phenotypic (intrinsic resistance to carbapenems) and molecular methods (amplification of 23S rRNA gene).

Results:Of the studied samples,186 (16.78%) nonfermentative gram negative bacilli (NFGNB) were identified. Amongst NFGNB, 18 (1.62%) isolates were identified as S. maltophiliaby using biochemical tests.Of 18 biochemically identified isolates, 15 (83.3%)were confirmed via PCR.Sinks (40%) and men surgery ward (33.3 %( were the most contaminated sites and wards of hospitals, respectively.

Conclusion:S. maltophilia is repeatedly isolated from sink which shows that the moist hospital environments need to be considered as a source for dissemination of bacteria.

Keywords:Nosocomial Infections,Nonfermentative Gram Negative,Stenotrphomonas Maltophilia, PCR

Corresponding Author: Douraghi, M.

Email: mdouraghi@tums.ac.ir

Received 7 July 2013 Revised 17 Jul 2013 Accepted 20 Jul 2013