This paper should be cited as: Bazzazi, H. Govahi, M. Jahazi, A. Alizadeh, Sh. Naeimi Tabiee, E. Mokaram, R. Davarpanah, M R. Medical Laboratory Journal, Autumn- Winter 2012, Vol.6, No.2.[Article in Persian]

Comparison the Anti-β2GP1 Autoantibody Level in Serum of Pregnant Women with History of Recurrent Miscarriage And Healthy Women in Gorgan

Bazzazi, H. (MSc) *1, Govahi, M. (MD) 2, Jahazi, A. (MSc) 3, Alizadeh, Sh. (MD) 4, Naeimi Tabiee, E.(MD) 5, Mokaram, R. (MA) 6, Davarpanah, M R. (BSc) 7

1 Instructor of Immunology, Department of Laboratory Sciences, Gorgan Branch of Islamic Azad University, Gorgan, Iran.

2 Gynecologist & Obstetrician, Department of Gynecology & Obstetrics, Hakim Jorjani hospital, Gorgan, Iran.

3Instructor of Midwifery, Department of Midwifery, Gorgan Branch of Islamic Azad University, Gorgan, Iran

4 Pathologist, Laboratory of Pathology, Hakim Jorjani hospital, Gorgan, Iran
5 Assistant  Professor, Department of Pathology, Golestan University of Medical Sciences, Gorgan, Iran

6 Instructor of Department of mathematics, Gorgan Branch of Islamic Azad University, Gorgan, Iran

7 Department of Laboratory Sciences, Gorgan Branch of Islamic Azad University, Gorgan, Iran.

Abstract

Background and objectives: Recurrent miscarriage (RM) is one of the most common reproductive disorders, generally considered to be the loss of three or more pregnancies before viability.  One of the causes of this disorder is the immunological factors such as autoantibodies associated with anti-phospholipid syndrome.

Material and Methods: this case-control study was conducted on 80 pregnant women divided into two equal groups of healthy and RM women in Gorgan. The subjects were asked to fill out a questionnaire and examined by a gynecologist to distinguish their condition, healthy or RM. The blood samples were kept at -20c and assessed by both ELISA 96 and automated ELISA processor with single dose ready-to-use test to measure β2GP1   autoantibodies of IgM and IgG.

Results: the results indicate that % 7.5  of RM patients are  seropositive for Anti-β2GP1 IgG, and % 5 for Anti-β2GP1 IgM , but in healthy pregnants ,  % 7.5 are  seropositive for Anti-β2GP IgG , and %7.5 for Anti-β2GP1 IgM. Family history of abortion in RM group (33.5%) is significantly higher than healthy one (17.5%).

Conclusion: based on the results, the difference between the level of Anti-β2GP1 IgM and Anti-β2GP IgG in case and control groups is not significant, but the production of this autoantibody is associated with pregnancy.

Key words: Autoantibody, Antiphospholipid syndrome, Recurrent Miscarriage

*Corresponding Author: Bazzazi, H. Email: hadi.bazzazi@gmail.com

Received 5 sep 2011  Revised 7 sep 2012 Acceppted 13sep 2012