This paper should be cited as: Ahmady- Abchin, S. Nasrolahi
Omran ,A. Jafari, N. Mostafapour M.J. Kia,S.M. Medical Laboratory Journal,
Autumn- Winter 2012, Vol.6, No.2.[Article in Persian]
Ahmady- Abchin, S. (MSc)1, Nasrolahi
Omran ,A.(PhD)*2, Jafari, N. (MSc) 3, Mostafapour M.J. (BSc)4, Kia,S.M.(BSc)5
1 Assistant professor, Department Biology,
Faculty of Science, Ilam University
2 Assistant professor,
Department of Microbiology, Faculty of Sciences, Islamic Azad University of
Tonekabon Branch, Tonekabon.
3 Assistant professor, Department of Biology, Faculty of science,
Mazandaran University
4 MSc Student of Biology, Department of Biology, Faculty of Science,
Ilam University
5 Msc Food Sciences of Technology Student, Islamic Azad University
of Azadshahr Branch
Antibacterial effects of Lavandula Stoechas Essential Oil,
on Gram Positive and Negative Bacteria In Vitro
Abstract
Background and Objectives: Concurrent with the development of new chemical
drugs and antibiotics,
their harmful effects are gradually emerged. Due to lack of harmful effects, herbal medicines have been
used in the pharmaceutical
industry. The aim of this study was the use of lavender essential oil as an herbal medicine for the replacement
of antibiotics and chemicals.
Material and Methods: In this study, the plant essential oil was isolated by drying
and distillation method using Clevenger apparatus. The antibacterial effect
of this plant was evaluated by using disc diffusion method and
successive dilutions. In order to control
the standard of the method, antibiotic discs and standard bacterial
strains were used.
Results: Based on the results, Proteus mirabilis and Enterococcus
faecalis are , respectively , the most sensitive and most
resistant bacteria to dilutions of 1, 1/2 and 1/4. Escherichia coli and Enterococcus faecalis, respectively, are the most sensitive and
most resistant bacteria
to the dilution of
1/8, 1/16, 1/32
and 1/64. MIC and MBC methods also show that all
bacteria have the same minimum inhibitory and fatality concentrations except Enterococcus
faecalis with minimum inhibitory concentration of 16/1 and minimum
concentration fatality of 8/1. Evaluating the results of the disk diffusion method with antibiotic
discs, we can observe the better effect of this plant in comparison
with gentamicin and streptomycin discs on the growth of five strains
of Staphylococcus aureus ATCC1885, Staphylococcus
epidermidis ATCC 2405, Enterococcus faecalis ATCC2321, Escherichia
coli ATCC 1652 and Proteus mirabilis ATCC 2601.
Conclusion: the essential oil of Lavender can be used instead of chemical drugs
to treat bacterial
infections.
Keywords: Lavandula, Anti-bacterial effects,
Essential oils, Bacterium
Corresponding Author: Nasrolahi omran ,A. Email: AYAT 51@ yahoo. Co. in
Received 2 Feb 2012 Revised 14 Nov 2012 Accepted 20 Nov
2012