Sedigheh Shirkhani, Alireza Barari, Asieh Abbasi Daloii, Mehrdad Saravi,
Volume 18, Issue 1 (Jan-Feb 2024)
Abstract
Background: Cardiovascular diseases, especially coronary artery problems, are the main causes of death. The aim of this study was to evaluate the expression of NOs and NOX2 in coronary artery patients after aerobic exercise and omega-3 intake.
Methods: The present study was a quasi-experimental study in which 32 men with coronary artery disease in the age range of 55 to 65 years were selected and randomly divided into 4 groups: control, exercise, omega-3, and omega-3 + exercise. The training program consisted of 8 weeks of intermittent running training, 3 sessions per week, with an intensity of 55 to 65% of the subjects' heart rate reserve and with an emphasis on gradual overload. Subjects consumed 1000 mg of omega-3 daily.
Results: There was a significant increase (P <0.0001) in the mean expression of the NOS gene in the exercise + omega-3 group compared to the control group. The mean ratio of NOX gene expression changes in the exercise group, omega-3, and the combination of exercise + omega-3 was significantly reduced compared to the control group (P <0.0001).
Conclusion: According to the results of the present study, the ability of exercise and omega-3 supplementation to reduce the level of oxidant stress and increase homeostasis control in coronary artery insufficiency shows an important molecular mechanism that underlies the benefits of these interventions.
Vahid Zolghadri , Alireza Barari , Asieh Abbasi Daloii , Hosein Abed Natanzi ,
Volume 19, Issue 5 (9-2025)
Abstract
Background: Melanoma is an aggressive malignancy that results from the transformation of pigment-producing melanocytes. This study aimed to investigate the effect of nettle extract consumption and aerobic exercise on the gene expression of IFN-γ and Endostatin in the liver tissues of mice with melanoma.
Methods: Twenty male BALB/c mice were randomly divided into four groups, including control, endurance, nettle, and endurance+nettle. The training program included running on a treadmill for 30 minutes at a speed of 16 meters per minute. The speed was increased by one meter per minute each week, reaching 22 meters per minute in the eighth week. Melanoma cells were injected subcutaneously into the left side of the mice. The experimental groups received 30 mg/kg/day of nettle ethanol extract orally for eight weeks. Real-time PCR was used to assess the expression of IFN-γ and Endostatin.
Results: IFN-γ expression levels in the experimental groups were not different from the control group, while Endostatin levels were significantly reduced (p = 0.142, p <0.001, respectively). IFN-γ expression levels in the experimental groups were higher than in the control group, but did not reach a significant level. Also, Endostatin expression levels in training and combination groups were significantly lower than in the control group (p = 0.022, p <0.001, respectively).
Conclusion: The results showed that endurance training combined with nettle extract may inhibit angiogenesis and capillary tissue formation in the tumor tissue of mice with melanoma by increasing IFN-γ and decreasing Endostatin.
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