Showing 3 results for Bakhtiari
Soltan Dallal Mm, Rahimi Forushani A, Bakhtiari R,
Volume 6, Issue 1 (spring-summer[PERSIAN] 2012)
Abstract
Abstract Background and objectives: Helicobacter pylori is a helical gram negative bacterium with polar flagella, discovered by Warren and Marshall in 1983. Helicobacter pylori exist in the stomach mucus tissue of less than 20% of people under 30 years old, but this amount would increase up to 40% and 60% in 60- year- old people. The aim of this study was to compare three methods of culture media, direct slide staining and the urease test for the rapid diagnosis of bacterium in case of peptic or duodenal ulcer. Material and Methods: In This descriptive study, duplicate biopsy specimens were taken from 82 clients referring to four different Hospitals .In endoscopy room of the Hospitals, a rapid urease test were carried out on one of duplicate specimens for the presence or non-presence of Helicobacter pylori. In order to see the Helicobacter pylori in the tissues, three slides using foushin, giemsa, and gram staining were prepared from the second specimens. Then, the specimens were incubated into selective culture media and incubated for 4-6 days in micoraerophilic condition. Results: Of 82 tested specimens 70(85.5%) and 66(80.5%) are identified as Helicobacter pylori by positive urease and culture medium, respectively. The frequency of foushin, giemsa, and gram staining are 67 (81.7%), 66 (80.5%), and 61 (74.4%), respectively. The foushin staining is the best with 100% sensitivity among the other methods. Conclusion: Based on difference between proportions, There is no significant difference between staining methods (foshin, giemsa, gram staining) and culture media in all cases. Key words: Helicobacter pylori, microscopic methods, urease test, culture media, identification
Mohammad Arefi, Abbas Abdollahi, Ayyoob Khosravi, Abdolavahab Moradi, Seyed Hamid Aghaee-Bakhtiari, Naimeh Javid, Mehdi Evazalipour, Anvarsadat Kianmehr,
Volume 15, Issue 2 (Mar-Apr 2021)
Abstract
Background and objectives: Colorectal cancer (CRC) is one of the leading causes of cancer-related mortality in the world. MicroRNAs (miRNAs) have potential as diagnostic biomarkers for various diseases including cancer. This study was undertaken to investigate expression of miR-21 before and after surgery in patients with hereditary CRC.
Methods: After collecting blood samples from 39 patients and 39 healthy controls, total RNA was extracted by the TRIzol method. Following cDNA synthesis, expression of miR-21 in serum of subjects was evaluated using real-time PCR, along with two reference genes, let-7d and let-7g. The real-time expression results and Ct values were collected and analyzed based on the 2-∆∆ct method.
Results: In spite of tumor removal, serum miR-21 expression levels was significantly higher in hereditary CRC patients compared with controls (P=0.022).
Conclusion: Our results confirmed that samples from hereditary cases of CRC must not be included in experiments on the diagnostic potential of miRNAs.
Ali Ehsan Shahbazi, Nastaran Barati, Eissa Soleymani, Pegah Khandan Del, Abolfazl Khandan Del, Nemat Azizi , Behjat Ranjouri , Mehran Bakhtiari , Seyedmousa Motavallihaghi ,
Volume 17, Issue 6 (Nov-Dec 2023)
Abstract
Background: COVID-19 is a respiratory disease pandemic and a major global health problem that can cause acute respiratory distress syndrome (ARDS). Previous studies have shown an inverse link between toxoplasmosis and COVID-19. This study aimed to evaluate the association between COVID-19 and toxoplasmosis infection.
Methods: In this cross-sectional descriptive study, samples were taken from 360 patients, 50% of whom were men and 50% were women. Of the patients, 180 were determined to be COVID-19-positive by the ELISA kit, and 180 were in the control group. Some demographic characteristics, such as sex, age range, and occupation, were also recorded.
Results: This study was conducted on 180 COVID-19-positive patients, of whom 26.7% were in the 16-30-year age group, 25.1% were self-employed, and 31.7% had anti-Toxoplasma gondii antibodies. Among the 180 control patients, 21.1% had antibodies for T. gondii. Of the 57 patients who were co-infected with both COVID-19 and toxoplasmosis, men had higher infection rates (63.2%) than women (36.8%). There was a significant relationship between co-infections with COVID-19 and toxoplasmosis with occupation, but not with age.
Conclusion: The study found that people with toxoplasmosis infection have a 1.73 times higher risk of contracting COVID-19. The findings suggest that infectious agents could be a predisposing factor, possibly due to changes in cytokine levels.
