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Showing 3 results for Fallah,
Identification of Intestinal Microsporidia by Trichrome Staining and Calcofluor White Methods among Kidney- Transplanted Patients in Hamadan
Z Gharibi, F Daadras, M Maghsood, M Fallah,, M Saeedijam,
Volume 7, Issue 4 (winter[PERSIAN] 2014)
Abstract
Abstract Background and Objective: Microsporidia is an obligatory intracellular parasite known as an opportunistic infection in immunocompromised patients. Its laboratory diagnosis is relatively difficult and modified trichrome staining is a standard diagnostic method for detection of Microsporidia. The aim of present study was to identify intestinal Microsporidia in kidney- transplanted patients, using modified Trichrome Staining and Calcofluor White Methods. Material and Methods: In 2012, 180 stool specimens were taken from kidney transplanted- patients given immunosuppressive drugs in Hamadan, Iran. To identify Microsporidia we use modified Trichrome Staining and Calcofluor White Methods. Results: The mean duration of kidney transplant and immunosuppressive drug using was 5.5 Years. Only one female patient was positive for Microsporidia. Conclusions: Owing to low frequency of this opportunistic infection among kidney transplanted patients, we can conclude that their hygienic conditions are good enough and they are not exposed with the parasites. Key words:Microsporidia, Modified Trichrome Staining,Kidney Transplant, Calcofluor White, Hamadan
Antimicrobial Resistance Pattern and Prevalence of Class 1, 2, and 3 Integrons in Clinical Isolates of Klebsiella Pneumoniae in Loghman-E Hakim Hospital, Tehran
Derakhshan, S, Najar Peerayeh, Sh, Fallah, F, Bakhshi, B,
Volume 8, Issue 4 (supplement Issue[PERSIAN] 2015)
Abstract

Abstract Background and Objective: Multiple drug resistance has increased in recent years in Klebsiella pneumoniae isolates. The Integrons are mobile genetic elements that carry antibiotics resistance genes. The aim of this study was to determine antibiotic susceptibility and the prevalence of class 1, 2, and 3 integrons in clinical Klebsiella pneumoniae isolated from clinical specimens. Material and Methods: A total of 108 K. pneumoniae isolates were collected between April and December 2011 from different clinical specimens of Loghman hospital in Tehran and identified by biochemical tests. Susceptibility of isolates to 14 antibiotic disks was determined by disk diffusion method. The template DNA was extracted by freeze-thaw method and the presence of class 1, 2, and 3 integrons was investigated by PCR method. Level of resistance to antibiotics in integron-positive and integron-negative isolates was determined. Results: The highest level of resistance was seen for cefotaxime, ceftriaxone, and amoxicillin-clavulanic acid (55.5%). In 79 isolates (73.14%) class 1 integron and in 57 of 79 isolates (72.15%) resistance to at least two classes of drugs were seen. The class 2 and 3 integrons were not detected. Among integron-negative isolates, 8 isolates (27.58%) had resistance to at least one antibiotic. Conclusion: The prevalence of class 1 integron in resistant K. pneumoniae is high therefore, the monitoring of drug resistance and limiting the use of antibiotics are necessary. Keywords: Klebsiella Pneumoniae, Integron, Multi-Drug Resistance


Study of zoonotic intestinal helmiths of alimentary tract of rodents in Hamadan, 2012
Moradi, M, Matini, M, Mohemmi, N, Maghsoud, A, Zahirnia, A, Parsa, F, Fallah, M,
Volume 9, Issue 2 (may,jun 2015[PERSIAN] 2015)
Abstract

Abstract

Background and Objective: Assessment of alimentary tract helminthes of rodents has a great zoonotic importance.  This study aimed at determining the prevalence of helminth infections in rodents in Hamadan.

Material and Methods: a total of 100 rodents trapped from different parts of city were transported to laboratory. After anesthetizing by chloroform, the animals were undergone an operation to isolates the helminthes. The isolates were stained by Carmine and identified at the genus and species levels. Furthermore, age, sex, weight of rodent and size of various organs of body were determined.

Results: Totally, 62% of the rodents were infected to intestinal helminthes. All trapped rodents were Rattus norvegicus. Six species of helminthes, including three Nematode (45%), 3 Cestode (51%) and no Trematode were isolated from rodents. The infection rate for different helminthes was as follow: Hymenolepis nana 21%, H. diminuta 29%, Heterakis spomosa 43%, Strongyloides sp. 1% Trichuris muris 1% and Cysticercus fasciolaris 1%.

Conclusion: in this area, infection rate of alimentary tract helminthes in the Rattus norvegicus, especially zoonotic helminthes, is relatively high, and the rate of Cestodes is higher than those of Nematodes and Trematodes.

Key words: Prevalence, Helminthes, Alimentary tract, Rodents


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