Showing 3 results for Haddadi
Mahboobi, R, Fallah Mehrabadi, J, , Pourmand, Mr, Mashhadi, R, Haddadi, A,
Volume 8, Issue 5 (winter[PERSIAN] 2015)
Abstract
Abstract Background and Objective: Increased antibiotic resistant strains and inadequacy of current vaccines against pneumococcal infections necessitate the study of novel protein antigens. It seems that minor autolysin of Streptococcus pneumoniae may have antigenicity. Thus, we aimed at cloning its gene for the first time. Material and Methods: After DNA extraction of Streptococcus pneumoniae (ATCC 49619), Specific primers were designed for amplifying minor autolysin gene fragment, using PCR. The purified gene fragment was inserted into pET21a vector and was transformed into bacterial competent cells by heat shock technique. The presence of gene and absence of mutation in the recombinant vector were checked out with sequencing and enzymatic digestion methods. The gene sequence was finally analyzed by bioinformatic tools. Results: The gene of minor autolysin was cloned successfully and the result of enzymatic digestion was the indication of complete isolation of this gen from plasmid. . Bioinformatics studies revealed that the mature protein was lacking signal peptide and the gene encoded 318 amino acids with a molecular weight of 36.4 kDa. Conclusion: The presentation and characterization of novel antigens such as minor autolysin could help us with finding new approaches for preventing and controlling pneumococcal infection. Keywords: Streptococcus Pneumoniae, Minor Autolysin, Cloning
Parisa Hasanein , Mehrnush Sotudeh , Mousa Bohlooli , Mohammad Haddadi,
Volume 19, Issue 6 (Nov-Dec 2025)
Abstract
Background: DNA glycation damages DNA by inducing strand breaks, mutations, and ultimately changes in gene expression, which is considered a main factor in the pathogenesis of diabetes and its complications. Therefore, antiglycation agents have become the focus of recent research for preventing and alleviating diabetes complications. According to the reported antidiabetic effects of Artemisia sieberi (A. sieberi) leaf extract, this study aimed to determine the effect of the ethanolic extract of A. sieberi on glucose-mediated DNA glycation for the first time.
Methods: DNA was incubated with glucose in the presence or absence of A. sieberi for 4 weeks. The inhibitory or facilitatory effects of A. sieberi on DNA structural changes were studied by various techniques. These techniques included UV–Vis, fluorescence spectroscopy, circular dichroism (CD), and agarose gel electrophoresis.
Results: The findings of UV–Vis and fluorescence spectroscopy showed that A. sieberi decreased DNA-AGE (Advanced glycation end products) formation. Based on the CD and agarose gel electrophoresis results, the structural changes of glycated DNA were decreased in the presence of A. sieberi.
Conclusion: Thus, A. sieberi has beneficial effects against DNA glycation and could be a promising agent for ameliorating the adverse effects of glycation in the presence of glucose and in conditions of raised blood glucose, such as diabetes, after confirmation in further studies.
Bahareh Behfar, Fatemeh Haddadi, Mohammad Reza Sharifmoghadam, Hossein Kamaladini, Masoumeh Bahreini, Azadeh Niknejad,
Volume 20, Issue 1 (1-2026)
Abstract
Introduction: The poultry industry produces a large amount of waste, including chicken feathers, which are difficult to decompose and can cause environmental pollution. Keratinase enzymes, which can break down keratin, have the potential to be used in bioremediation of poultry waste. The objectives of this study were to screen for keratinolytic isolates from poultry waste, Identify the isolates using morphological, biochemical, and molecular methods; optimize the culture medium conditions for keratinase production and measure the keratinase activity of the isolates.
Materials and Methods: Two keratinolytic isolates were screened from poultry waste around Mashhad in Iran. The isolates were identified using morphological, biochemical, and molecular methods. The culture medium conditions for the two strains were optimized to enhance keratinase production. The keratinase activity was measured using azokeratin substrate and turbidity absorbance.
Results: The two isolates were identified as Bacillus pumilus and Bacillus teqlensis. The optimized culture medium conditions for keratinase production were pH 7.0, temperature 37°C, and incubation time 48 hours. The maximum keratinase activity of the two isolates was 120 U/mL and 100 U/mL, respectively.
Conclusions: The two Bacillus isolates have the potential to be used in bioremediation of poultry waste. The optimized culture medium conditions can be used for large-scale production of keratinase enzymes.
The keratinase enzymes produced by the two Bacillus isolates have the potential to be used in a variety of applications, including bioremediation of poultry waste, production of animal feed, and development of new cleaning products.
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