Abstract

Background and Objective: Nosocomial infection is one of the main factors of mortality in hospitals . One of the most important measures to control infection in hospitals  is sampling of equipment and  culturing the samples . This study aimed to investigate the prevalence of gram-negative bacilli obtained from the equipment of the hospital wards .

Material and Methods: This Descriptive study was carried out on 488 specimens taken from medical and non-medical devices and equipment of various wards, via census sampling. the samples taken by sterile swabs in Tryptic Soy Broth (TSB) were inoculated in blood agar and Eosin-Methylene Blue agar, and by biochemical standard tests the isolated colonies  were identified.

Results: Of 488 isolates, 157 (32.17%) were positive for the presence of gram-negative bacilli. The highest bacterial contamination was related to Enterobacter (37.58%). Of  hospital wards, the highest infection related to the surgical wards (19.75%), and the highest contamination is related to laryngoscope blades (12.74%) and ECG sensor device and the monitoring interface (7%).

Conclusion: Microbial infection with gram-negative bacilli  is an overwhelming condition  in hospital equipment.

Key Words: Gram Negative Bacilli, Nosocomial Infection, hospital wards, hospital equipments, Gorgan

Abstract

Background and Objective: Bacterial infections are of the leading causes of morbidity and mortality in ICU patients with underlying immunodeficiency. This study aimed to determine the frequency of microorganisms in patients admitted to the ICU of Panje Azar Hospital in Gorgan.

Material and Methods: This cross-sectional study was conducted on the patients admitted in three wards, each having at least 48, of ICU in Panje Azar Hospital in Gorgan. All microbial cultures requested by patients' physician were recorded.

Results: In ICU positive cultures were 53.9%.  The number was 16 for Staphylococcus coagulase negative,  15 for  Enterococci  , 11 for Yeast, 11 for E.Coli, 9 for Enterobacter  , 8 for streptococcus, 5 for staphylococcus aureus. The highest  percentage was related to coagulase-negative staphylococci (16.5%) and the lowest to Alcaligenes, Diphtheroids, Acremo bacteria , Serratia with one positive case.

Conclusions:  the incidence of bacterial contamination in ICU patients compared to other parts of country is not different significant.  While it is far from world standard, it is essential that many efforts be done to reduce the level of infection.

Keywords: ICU; Microorganisms; Panje Azar Hospital.