Mr Kiaei, M Hedayatmofidi, F Koohsar, A Amini, S Hoseinzadeh, A Mirbazel, Z Hesari,
Volume 8, Issue 1 (spring[PERSIAN] 2014)
Abstract
Abstract
Background and Objective: C - reactive protein (CRP) is an acute phase protein produced in liver. It is less than 5 mg per deciliter in the serum and body fluids of normal individuals, but it is increased suddenly within a few hours following inflammatory reaction. In bacterial and viral infections, active rheumatic fever, acute myocardial infarction and rheumatoid arthritis are also increased. The aim of this study was to investigate CRP level by Qualitative and Quantitative methods.
Material and Methods: The CRP of 200 patients was investigated by quantitative and qualitative methods. Qualitative CRP testing was conducted three times by different people, using two kit of bionic and Omega, and then the mean of the results was reported. For quantitative CRP testing, Immunoturbidimetry was used.
Results: In qualitative CRP test by Bionic kit: 180 (90%) were negative, 6 (3%) weakly positive, 9 (4.5%) +1 and 5 (2.5 %) were + 2. In qualitative CRP test by Omega Kit: 148 (74%) were negative, 32 (16%) weakly positive, 13 (6.5%) +1, 4 (2%) +2 and 3 (1.5%) were +3. A high percentage of Qualitative results, which were weakly positive, became negative by Quantitative methods. The Qualitative results of +1 and the next became positive by Quantitative methods.
Conclusion: It seems that in the early stages of inflammatory disease, quantitative methods are preferred to qualitative methods. Also, in cases that the CRP test results are weakly positive by qualitative methods, they should be controlled by quantitative methods too.
Keywords: CRP CRP Test Quantitative Qualitative CRP Test
Ayatollahi, Aa, Amini, A, Rahimi, S, Kiaei, M, Koohsar, F, Khandan Del, A,
Volume 9, Issue 2 (may,jun 2015[PERSIAN] 2015)
Abstract
Background and Objective: Nosocomial infection is one of the main factors of mortality in hospitals . One of the most important measures to control infection in hospitals is sampling of equipment and culturing the samples . This study aimed to investigate the prevalence of gram-negative bacilli obtained from the equipment of the hospital wards .
Material and Methods: This Descriptive study was carried out on 488 specimens taken from medical and non-medical devices and equipment of various wards, via census sampling. the samples taken by sterile swabs in Tryptic Soy Broth (TSB) were inoculated in blood agar and Eosin-Methylene Blue agar, and by biochemical standard tests the isolated colonies were identified.
Results: Of 488 isolates, 157 (32.17%) were positive for the presence of gram-negative bacilli. The highest bacterial contamination was related to Enterobacter (37.58%). Of hospital wards, the highest infection related to the surgical wards (19.75%), and the highest contamination is related to laryngoscope blades (12.74%) and ECG sensor device and the monitoring interface (7%).
Conclusion: Microbial infection with gram-negative bacilli is an overwhelming condition in hospital equipment.
Key Words: Gram Negative Bacilli, Nosocomial Infection, hospital wards, hospital equipments, Gorgan
