Showing 4 results for Lee
F Sedighian, A Sanee, H Alaouddoulee, M Arshi, Kh Rekabpoor,
Volume 2, Issue 2 (Autumn – Winter 2009[PERSIAN] 2008)
Abstract
Abstract Background and objectives: Resistance to antimicrobial agent is a world-wide problem and the existed reports are not the same. Aware of antibiotic resistant pattern of microorganisms in each hospital is important for controlling the nosocomial infections. This study was designed to determine the resistant pattern of microorganisms isolated in Yahya nejad hospital, Babol, north of Iran. Material and Methods: In this descriptive study, organisms were isolated during 2006 from urine, blood, endotracheal tube, abscess, fluids (ascites, pleura, and synovia), wound, discharges and bonemarrow of both outpatients and inpatients in our hospital were studied. Their antibiotics resistances were evaluated by Kirby Bauer method. Results: of 3114 culture specimens of admitted patients (female 1732, male 1382), 325 (10.4%) microorganisms were isolated. They were isolated from urine 162(49.8%), blood 115(35.4%), abscess 11(3.4%), wound, 10(3.1%) and discharge10 (3.1%). The most common microorganisms were Escherichia coli 107(33%) and Staphylococcus epidermidis 69(21.3%). Maximum resistance of E.coli is related to tetracycline (83.9%) and ampicillin (75%) and maximum resistance of S.epidermidis to oxacillin (89.2%) and penicillin (82.1%). Conclusion: With regard to high antibiotic resistance (>70%) in this study, it seems that the early beginning and inappropriate dosage of antimicrobial agents may be associated with developing antibiotic resistance. Hence, it is highly recommended to prohibit the unnecessary prescription of antibiotics Key words: Antibiotic resistance, microorganisms, Blood culture, E.coli
Ifrah Jan, Rafiqa Eachkoti, Iqra Farooq, Sanah Farooq, Sadaf Saleem, Sabhiya Majid ,
Volume 18, Issue 1 (Jan-Feb 2024)
Abstract
Background: The complete blood count (CBC) profile has been found extremely useful in monitoring the growth of SARS-CoV-2 infection; however, predictive CBC parameters that could be used in the management of the disease may vary in different populations.
Methods: This study comparatively analyzed the CBC profile of SARS-CoV-2 patients (N = 75; confirmed positive by real-time polymerase chain reaction [PCR]) and healthy individuals (confirmed negative by real-time PCR) from Kashmir (north India).
Results: Compared with healthy individuals, most of the CBC parameters (hemoglobin levels [13.43 vs 10.9 g/dL; P = 0.0001], lymphocytes [16.04% vs 30.8%; P = 0.00001], monocytes [5.53% vs 7.53%; P = 0.009], and platelet count [150 vs 186 ×103 µL; P = 0.037]) were significantly low in SARS-CoV-2 infected patients, while neutrophilia was more common in infected patients (76.77% vs 59.26%). Among derived parameters, the neutrophil-to-lymphocyte ratio (NLR; 7.31 vs 2.04; P = 0.001) and derived NLR (d-NLR; 4.43 vs 1.5; P = 0.0002) were significantly high in SARS-CoV-2 patients. Further correlation analysis revealed a significant association of neutrophilia with the severity of the disease in SARS-CoV-2 infected patients. Moreover, receiver operating characteristic (ROC) analysis of derived CBC parameters (NLR, d-NLR, and platelet‐to-lymphocyte ratio [PLR] with disease severity and disease outcome) revealed d-NLR as better predictive marker of disease severity (area under the curve [AUC] = 0.658) and disease outcome (AUC = 0.766) compared to PLR with disease severity (AUC = 0.645) and disease outcome (AUC = 0.693).
Conclusion: We therefore conclude, of the CBC parameters neutrophilia as the marker of disease severity and among derived parameters, d-NLR as an early predictive biomarker of both disease severity and poor disease outcome in SARS-CoV-2 patients.
Adrian Yong Sing Lee,
Volume 20, Issue 1 (1-2026)
Abstract
Background: Autoimmune liver diseases (ALD) are a heterogeneous group of disorders affecting the hepatobiliary system and are characterized by specific autoantibodies. These are routinely measured in diagnostic laboratories using commercial line immunoblot (LIB) assays. However, the ordering characteristics and diagnostic performance of this test have not been extensively evaluated. This study aims to examine the performance of the ALD LIB in a single diagnostic laboratory.
Methods: A retrospective, cross-sectional audit of 12 months of data was performed at the Institute of Clinical Pathology and Medical Research diagnostic laboratory (Westmead Hospital, Australia). Patients referred for an ALD-LIB were included. Medical notes were reviewed to ascertain the clinical diagnoses of patients. Patients who had at least one positive ALD autoantibody on LIB were defined as “blot-positive” and compared with “blot-negative” patients. The performance of the ALD-LIB was assessed through the calculation of diagnostic sensitivities and specificities.
Results: There were 611 patients included over the 12-month period. Sixty-four of these patients (10%) were blot-positive. These patients were more likely to be female, to have other ALD-associated autoantibodies, and to have lower alkaline phosphatase (ALP) levels compared with blot-negative patients. An ALD diagnosis or systemic autoimmune disease was more likely to be identified in blot-positive patients. Finally, the LIB demonstrated a high negative predictive value for an ALD diagnosis in this patient cohort.
Conclusion: This real-world analysis of the laboratory’s ALD-LIB provided insights into the ordering characteristics and performance of this assay in patients referred for testing. When combined with other ALD investigations, the ALD-LIB is a useful adjunct in the evaluation of patients with suspected ALD.
Arulananthi V.a, Sabarinathan T, Saleem M,
Volume 20, Issue 1 (1-2026)
Abstract
Background: Burkholderia pseudomallei is a saprophytic, aerobic, non-sporing, nonfermenting, facultative intracellular motile gram-negative bacilli found in tropical and subtropical regions. Melioidosis can range from asymptomatic infections to life-threatening illnesses affecting the internal organs, particularly when the host's immunity is compromised. Delayed clinical presentation and a lack of access to critical care resources in high-prevalence areas significantly contribute to this death burden. This disease has a high case fatality rate in endemic areas. The written consent was obtained from the patient after explained them about this study. To increase physician awareness of melioidosis in high-risk patients presenting with diverse clinical symptoms, thereby enhancing diagnostic accuracy and ensuring timely, appropriate treatment. Case descriptions:Case series: Case 1: 40-year-old male with septic pulmonary emboli; Case 2: 72-year-old female with chronic obstructive pulmonary disease and Type II respiratory failure; Case 3: 25-year-old female with septic arthritis; Case 4: 69years-old male with pneumonia and bacteraemia; Case 5: 54-year-old male with diabetic nephropathy and chronic kidney disease. The appropriate samples were collected from all the cases, and the identification of the isolated organisms and antibiotic susceptibility tests were performed using Vitek 2 automated compact system. The patients were treated with appropriate supportive measures. Among the five cases, three had a better outcome, and two cases had deteriorated. Conclusion:This case series emphasis that the Clinicians should be aware of the risk of melioidosis, particularly during the rainy season, Diagnostic tests should be enhanced to ensure timely exploration and treatment.
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