Abstract Background and objective: Exposure of red blood cells to oxygen radicals can induce Lipid proxidation, hemoglobin damage and hemolysis of erythrocyte .The present study was designed to determine the alteration of plasma lipid peroxidation and erythrocyte Superoxide Dismutase and Glutathione Peroxidase enzyme activities in stored blood and to find out the quantitative alterations and the useful length of stored blood. Materials and Methods: First, the whole blood form 10 donors was taken. Then Red Blood Cells(RBC) were counted, the levels of Potassium(P) and lactate dehydrogenate activity(LDH) were measured to determine the amount of hemolysis, the plasma levels of malondialdehyde (MDA), erythrocyte Superoxide Dismutase(SOD) and Glutathione Peroxidase(GPx) were studied for determination of lipid peroxidation and antioxidant enzyme activities at the days of 0,1,3,5,7,9,11,13,15,17,19,21,23,25,27,29,31,33 and 35 of the storage. Results: upon storage time, the plasma levels of malondialdehyde (MDA) and Potassium and lactate dehydrogenate activity increased (P< 0.05) whereas erythrocyte Superoxide Dismutase and Glutathione Peroxidase enzyme activities and Red Blood Cells decreased (P< 0.05). The alterations of MDA, SOD, GPx, P, LDH and RBC in the measurement days were as follows: MDA, P and LDH significantly increased at the day of 9, 5 and 5 whereas SOD,
GPx and RBC decreased at the day of 11, 7 and 29 respectively. Conclusion: The results of this study showed that the increased level of MDA and decreased SOD and GPx in stored blood can cause the beginning of hemolysis of erythrocyte therefore, it is necessary to control these factors before storing the donated blood. Keywords: lipid peroxidation, Superoxide Dismutase, Glutathione Peroxidase

Abstract

Introduction:

methionine. Many reports confirm the correlation between hyper

homocysteinemia and cardiovascular disease. This study was aimed

at determining the effect of B12 and folate deficiency on the

homocysteine level after myocardial infarction.

Materials and methods:

study were patients with myocardial infarction (N = 48) and healthy

patients (N = 48) Eliza method was used to assay Homocysteine and

RIA for folic acid and vitamin B12.

Results:

(30.3 ± 5.3 μm/l) and the control group (11.1 ± 3.1) is significant (p<

0.001). There is no significant difference between Serum B12 in case

(297.1 ± 208.9 pm/l) and control group (261.5 ± 205.3) and it is true

about Serum folic acid of case (3.9 ± 2.9 ng/m) and control group

(4.3 ± 3.5). The homocysteine level of all patients and four of

healthy subjects is higher than normal. The folic acid Level of 11

patients and four healthy subjects is less than normal.

Conclusion:

of control group and this difference is not related to decrease of B12

Level, Physicians must pay attention to The other risk factors.

Key words:

cobalamine, cardiovascular disease.