Showing 3 results for Mesgarian
Ar Niazi, F Koohsar, F Ghaffarifar, H Ziaei-Hezarjaribi,, F Mesgarian, on Jorjani,
Volume 8, Issue 2 (summer 2014[PERSIAN] 2014)
Abstract
Abstract
Background and Objective: Culture, microscopic method is a gold standard method for identification of Lishmania parasite. The use of Molecular methods such as RT- PCR compared to microscopic methods has a higher sensitivity and specificity however, it is not widely used due to its expensive equipment and the time requested. The use of nucleic acid sequence based amplification (NASBA) method is highly valuable for diagnosis of live parasite because there is no need for to use Thermo cycler. We aimed to assess sensitivity and specificity of NASBA for molecular detection of cutaneous Leishmaniasis.
Material and Methods: First, the RNA was extracted from 28 skin biopsies suspected cutaneous Leishmaniasis. Then, by means of specific primers designed for 18srRNA region, this region was amplified using NASBA isothemal amplification. To increase the sensitivity, the product was electroforesed in TBE (IX) buffer, using Syber Gold Flourecent probes. Using specific primers, RT- PCR was conducted on the samples too.
Result: For diagnosis of Leishmania parasites, NASBA and RT-PCR had the sensitivity of 81% and 51%, respectively, and specificity of 100%.
Conclusion: NASBA isothermal method with high sensitivity and specificity can be applied for identification of cutaneous leishmaniasis.
Keywords: Cutaneous Leishmanisis, NASBA, 18S rRNA
Mesgarian, F, Sharbatkhori, M, Mohammadi, R, Rajabi, Mh,
Volume 8, Issue 5 (winter[PERSIAN] 2015)
Abstract
Abstract Background and Objective: Cryptosporidium is a common protozoan causing diarrhea in human, specifically in children. Hence, we aimed to investigate the prevalence of this protozoan among diarrheic children hospitalized in Gonbad Kavus in 2011. Material and Methods: Three stool samples were collected from diarrheic children in two hospitals of Gonbad city and a relevant questionnaire was filled out for each child. The stool samples were concentrated by formalin ether method, and the infection was assessed by modified acid-fast staining method. Results: Of 547 children, 27 (4.9%) were infected with cryptosporidiosis. There was no significant relationship between the amount of infection and gender and habitation area (urban/ rural). The infection rate was significantly prevalent in 2-4-year-old children (P=0.013). The most and the least infection rate were observed in spring and winter, respectively (P< 0.0001). There was a significant association between the disease and keeping animal (P= 0.041) Conclusion: The prevalence of cryptosporidiosis in diarrheic children in Gonbad is almost equal to other regions of the country and keeping animal and spring season may be considered as the risk factors for the disease. Keywords: Cryptosporidium, Cryptosporidiosis, Diarrhea, Children, Golestan, Iran
Afieh Samimi, Oghol Niaz Jorjani, Zohreh Sharifi, Faramarz Koohsar, Khodaberdi Kalavi, Fatemeh Mesgarian, Beniamin Talebi ,
Volume 16, Issue 3 (May-Jun 2022)
Abstract
Background and objectives: Cutaneous leishmaniasis is endemic in most areas of Iran, and the diagnosis of its species is essential for controlling the disease. Leishmania major is the causative agent for cutaneous leishmaniasis in humans. Molecular methods are generally more sensitive than microscopic methods. The present study aimed to use a polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA) technique for detecting live L. major from wounds of patients with cutaneous leishmaniasis.
Methods: In the present study, a standard strain of L. major promastigotes was used as the positive control for purification of DNA. The Novy–MacNeal–Nicolle and RPMI-1640 media were used for reproduction of parasites. DNA was isolated from specimens taken from 35 patients with suspected cutaneous leishmaniasis whose disease was confirmed by direct smear method. The PCR-ELISA technique was later applied by using the standard strain, patient specimens, and primers specific for the 18s rRNA.
Results: Out of 35 patients, 17 (48.6%) were male and 18 (51.4%) were female. In addition, 8.6% of the patients lived in the Gonbad-e Kavus County, while all patients had been infected in villages around Gonbad-e Kavus. Of 35 patients with confirmed cutaneous leishmaniasis according to the direct smear method, 31 patients (86.31%) had leishmaniasis based on the PCR method and the PCR-ELISA methods.
Conclusion: Based on the results, the PCR-ELISA method is more sensitive and accurate for detecting L. major.
