Showing 5 results for Mohammadian
M Mohammadian Yajloo,, A Sahebgadam Lotfi, M Nasroallahzadeh Sabet, N Zhalehjoo, M Amirian, M Biglarzadeh,
Volume 1, Issue 1 (Spring - Summer 2007 [PERSIAN] 2007)
Abstract
Abstract Background & Objective: Alpha-1-antitrypsin (AAT) is the major component of the human plasma alpha-1 globulin proteins and acts as a major inhibitor of proteolytic enzymes, particularly elastase. AAT deficiency is accompanied by lung, liver and other disorders, therefore, AAT is clinically important and its precise evaluation is diagnostically critical. In present study serum AAT was evaluated by three Cellulose Acetate Electrophoresis (CAE), Trypsin Inhibitory Capacity (TIC) and Single Radial Immunodiffusion (SRID) methods and results wene compared. Materials and methods: AAT evaluation was carried out, by CAE, SRID and TIC Methods, on 318 normal sera obtained from volunteer students of Tehran Universities. Results:The results indicated: 34, 84 and 112 samples by TIC, SRID and CAE methods (with reference ranges of 2.1-3.5 mol/min/ml, 126- 226 mg/dl and 2-4.5% respectively) were abnormal 201samples by CAE and TIC were normal and 29 abnomal, 83 sera were normal by TIC and abnormal by CAE five of them were abnormal by TIC and normal by CAE 227 of the samples were normal and 29 abnormal(TIC and SRID) 57 were normal by TIC and abnormal by SRID and seven samples were abnormal by TIC and normal by SRID. Conclusion: Although CAE and alpha-1 globulin band determination are routine in clinical laboratory, they are not reliable in evaluating AAT. SRID sensitivity is more Than CAE and less Than TIC therefore, TIC is recommended as a precise and reliable method for serum AAT evaluation. Key Words: Alpha-1-antitrypsin, Cellulose Acetate Electrophoresis, Single Radial Immunodiffusion, Trypsin Inhibitory Capacity
Mt Badele, M Mirzaian, M Babaei, B Badele, F Derakhshan Pour, S Mohammadian,
Volume 8, Issue 5 (winter[PERSIAN] 2015)
Abstract
Abstract Background and Objective: With regard to high prevalence of attention deficit hyperactivity disorder (ADHD) and its being significantly affected by nutritional factors, we aimed to determine the relationship between Ferritin serum level and ADHD. Material and methods: This ex-post- facto (causal comparative research) design study was conducted on 60 children, selected via convenience sampling. Thirty of them were ADHD children diagnosed by a psychiatrist using DSM IV checklist, as a case group, and the rest were healthy ones located in control group. Having their family informed consent, their Ferritin level was measured via ELIZA method. Results: The results show that Ferritin serum level of ADHD children are lower than that of healthy ones. Using t- test, it was indicated that the difference is significant (p= 0.002). Besides, the result of Pearson correlation coefficient showed that there is no significant relation between Ferritin and ADHD. Conclusion: In terms of the results and the importance of this issue, we recommend conducting further controlled research. Keywords: Ferritin, ADHD, Attention Deficit Hyperactivity Disorder
Badeleh, Mt, Mirzaian, M, Babaei, M, Badeleh, M, Derakhshan Pour, F, Mohammadian, S, Vakili, Ma, Charkazi, A,
Volume 9, Issue 3 (Jul,Aug2015[PERSIAN] 2015)
Abstract
Background and Objective: With regard to high prevalence of attention deficit hyperactivity disorder (ADHD) and its being significantly affected by nutritional factors, we aimed to determine the relationship between zinc serum level and ADHD.
Material and Methods: This ex-post- facto (causal comparative research) design study was conducted on 60 children, selected via convenience sampling. Thirty of them were ADHD children diagnosed by a psychiatrist using DSM IV checklist, as a case group, and the rest were healthy ones located in control group. Having their family informed consent, their Zinc level was measured via Colorimetric method.
Results: The results show that Zinc serum level of ADHD children are lower than that of healthy ones. Using t- test, it was indicated that the difference is not significant. Besides, the result of Pearson correlation coefficient showed that there is no significant relation between Zinc and ADHD.
Conclusion: given that the zinc level was lower in ADHD children, the difference was not significant. Therefore, we recommend conducting further research with a larger sample size.
Neda Gharagozloo Hesari, Davoud Esmaeili, Taher Mohammadian, Mohammad Hasan Shahhosseini, Atousa Ferdosi,
Volume 14, Issue 6 (Nov-Dec 2020)
Abstract
Background and objectives: Industrial wastewater is worldwide health concern. Microorganisms present in the environment have an important role in the biodegradation of lipids, fats and proteins from wastewater. In this regard, microbial lipases and proteases are interesting research targets because of high stability, broad substrate specificity, high yields and availability. In this study, we analyze sequences encoding lipase of Pseudomonas putida and subtilisin of Bacillus subtilis for generation of a new recombinant protein for degradation of environmental contaminations caused by lipids and proteins.
Methods: In this study, sequences of the genes encoding lipase and subtilisin were obtained from GenBank. To predict the 3D structure of the protein, modeling was carried out. The prediction of secondary structure, tertiary structure and solvent accessibility was carried using bioinformatics tools including I-TASSER, GoR4 and ExPasy.
Results: The lipase-subtilisin fusion protein was well-characterized by bioinformatical studies with appropriate spatial and secondary structures. The protein had appropriate hydrophilicity, biological half-life and thermal and acidic stability. The codon optimization was performed appropriately.
Conclusion: Overall, the bioinformatical analysis of the designed protein showed that the recombinant lipase-subtilisin protein has a stable structure both in vitro and in vivo, a negative normalized B-factor and lipolytic and proteolytic activities, which makes it suitable for treatment of lipid and protein contaminations.
Sepideh Khodamoradi, Mohammad Hassan Shahhosseiny, Taher Mohammadian, Atousa Ferdousi,
Volume 15, Issue 4 (Jul-Aug 2021)
Abstract
Background and objectives: Different environmental factors, such as infection, can cause Alzheimer's disease (AD). Herpes simplex virus types 1 (HSV1) and 2 (HSV2) and cytomegalovirus (CMV) are related to AD. This study explores the potential role of HSV1, HSV2 and CMV in AD progression.
Methods: Plasma samples were taken from 100 AD patients (47 women and 53 men). After isolating viral DNA, PCR was performed using specific primers for the detection of the viruses.
Results: The prevalence of CMV, HSV1 and HSV2 was 27%, 8% and 4%, respectively. Although CMV was most prevalent in AD patients, HSV1 and HSV2 were found in patients with advanced AD. The prevalence of HSV1 and HSV2 was significantly associated with dysphoria, hallucination, insomnia and depression (P˂0.05), while CMV was significantly associated with hallucination and dysphoria (P=0.001). AD symptoms were higher in patients with HSV1 and HSV2.
Conclusion: It seems that HSV and CMV infections may be related to the severity of AD.
