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Shahande Z, Sadighian F, Shafi H, Ebrahimnejad A,
Volume 5, Issue 1 (spring-summer[PERSIAN] 2011)
Abstract

Abstract Background and objectives: Infection stones are the directly due to persistent or recurrent infection with Urease producing bacteria. they may be exacerbated by urinary obstruction or stasis. Also, some drug components like ciprofloxacin can be the cause of stone formation. We aimed at studing antibiotic resistance of bacteria isolated form kidney stones and Urinary tract infection(UtI). Material and Methods: This descriptive study, during 2008-2010,we obtained kidney stones and urine of 45 patients under gone nephrolithotomy in shahid Beheshti hospital of Babol, Iran. The stones were transferred to microbiology Lab in a strile Condition, after grinding, cultured on Blood Agar and Eosin-Methylene Blue(EMB) media. In Addilion, urin Samples were cultured on the for mentioned media. The Positive cultures were qualitatively evaluated and then, antibiogram was done by using Kirby Bauer method. Results: The resulte show that 10 kidney stone specimens and 8 urine samples were infected by bacteria, mostly by E.coli. In one stone sample and two urine samples, we can isolate more than one bacteria. The bacteria isolated in the stone and urine samples of six patients was the same. We could find two cases of staphylococcus inurine and one in stone culture. The bacteria isolated from stone are resistance to oflaxacin(80%) , which is higher than that to the other antibiotics. Conclusion: Based on the results, there is no relationship between the kind of kidney stone and type of bacteria. Further study needs to be done to prove this relation. Key words: Kidney stone, Antibiogram, E.coli, Infected stone.
F Sadighian, Z Abedian, A Saane-Ei, Z Mirkarimi,
Volume 7, Issue 3 (Autumn 2013)
Abstract

Abstract Background and Objective: Nowadays, nosocomial infection is one of the greatest problems in hospitals. Normal flora of staff’s hands and the bacterial agents on the surface of medical equipment can become progressively colonized with potential pathogens during patient care. This study was carried out to determine the bacterial agents existed on staff’s hands and in the wards of hospital to step in to control nosocomial infection. Material and Methods: In this descriptive study, during 17 months (22.mar.2010- 30.aug.2011), 403 samples, using sterile swab , were randomly obtained from the staff’s hands and medical equipment of emergency departments , ICU, male operation room and female surgical unit . The samples were cultured on Blood agar (BA) and Eosin methylene blue (EMB). Then, identification of isolated bacteria was done with diagnostic tests. Results: Of 430 samples, 530 bacteria were isolated from staff’s hands (N= 291) and medical equipment (N= 234). The most common bacterium from personnel’s hands (144 49.5%) and medical equipment (24 10%) is Staphylococcus aureus. Also, three isolates of pseudomonas aeruginosa from staff’s hands of male surgical ward and medical equipment of ICU, and two isolates of Acinetobacter.spp from ICU’s medical equipment were identified. Conclusion: With regard to the findings, it seems that applying the appropriate disinfectant agents by using standard procedures is necessary. Keywords: Medical Equipment Staff’s Hand Nosocomial Infection Staphylococcus Aureus

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