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Abstract Background and objectives: Pseudomonas aeruginosa as an opportunistic pathogen can establish lethal infections in immunocompromised patients or those exposed to predisposing factors. This bacterium contains a single polar flagellum causing motility, chemotaxis and colonization in acute phase of infection. The flagella filament is made up of a structural protein called flagellin. This study was aimed at determining The frequency of fliC gene in Clinical Samples. Material and Methods: In this study, a pair of specific primer for types of flagellin (a, b type) was designed and by using PCR method its structural gene (fliC) was recognized and amplified in clinical strains. Results: This original primer has appropriate efficiency in diagnostic of pseudomonas aeroginosa flagellum. Our study shows that 85% of the Clinical Samples have a fliC gene. Conclusion: This method can be applied to recognizing of the motile strains, and their antigenic typing, and complete amplification of fliC sequence in order to cloning and expression of recombinant flagellin. Key words: Pseudomonas aeruginosa, flagellin, fliC, PCR

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ABSTRACT
        Background and Objectives: Infertility is one of the important causes of anxiety in couples. Infections caused by genital Mycoplasmas may have harmful effects on the reproductive health of women, and sometimes lead to infertility. This study was designed to determine frequency of anti-Mycoplasma hominis antibodies in infertile women at Al-Zahra Hospital, Tabriz, Iran.
       Methods: In this cross-sectional study, serum from 184 infertile women and 100 healthy pregnant women were tested for presence of M. hominis IgM and IgG antibodies by ELISA. Data collected were analyzed in SPSS (version 17) using t-test at significance level of 0.05.
         Results: The frequency of anti-M. hominis IgG was significantly higher in infertile women compared to healthy controls. The frequency of anti-M. hominis IgM did not differ significantly between the infertile and control subjects. The majority of the women infected with the bacterium were in the 21-30 and 31-40 years age range. There was no significant correlation between tubal factor infertility and seropositivity for M. hominis antibodies. Moreover, the dwelling of the participants had no relationship with the frequency of anti-M. hominis antibodies. 
        Conclusion: The frequency of owed that a high percentage of t a high percentage of that the meaninngful le women was 4% and infertile women infected with this bacterium is high in Tabriz. Therefore, it is important to perform microbial screening for this bacterium in infertile couples. 
       Keywords: Mycoplasma hominis, Infertility, Women, Tabriz.
ABSTRACT
        Background and Objectives: Infertility is one of the important causes of anxiety in couples. Infections caused by genital Mycoplasmas may have harmful effects on the reproductive health of women, and sometimes lead to infertility. This study was designed to determine frequency of anti-Mycoplasma hominis antibodies in infertile women at Al-Zahra Hospital, Tabriz, Iran.
       Methods: In this cross-sectional study, serum from 184 infertile women and 100 healthy pregnant women were tested for presence of M. hominis IgM and IgG antibodies by ELISA. Data collected were analyzed in SPSS (version 17) using t-test at significance level of 0.05.
         Results: The frequency of anti-M. hominis IgG was significantly higher in infertile women compared to healthy controls. The frequency of anti-M. hominis IgM did not differ significantly between the infertile and control subjects. The majority of the women infected with the bacterium were in the 21-30 and 31-40 years age range. There was no significant correlation between tubal factor infertility and seropositivity for M. hominis antibodies. Moreover, the dwelling of the participants had no relationship with the frequency of anti-M. hominis antibodies. 
        Conclusion: The frequency of owed that a high percentage of t a high percentage of that the meaninngful le women was 4% and infertile women infected with this bacterium is high in Tabriz. Therefore, it is important to perform microbial screening for this bacterium in infertile couples. 
       Keywords: Mycoplasma hominis, Infertility, Women, Tabriz.