Farid Soltani , Saman Mahdavi ,
Volume 12, Issue 6 (Nov - Dec 2018)
Abstract
ABSTRACT
Background and objectives: Bacillus licheniformis is a potential cause of spoilage in pasteurized products. The aim of this study was to identify and isolate B. licheniformis from commercial pasteurized fruit juices distributed in the West Azarbaijan Province, Iran.
Methods: Sixteen fruit juice samples including four apple juice and 12 orange juice samples were collected from five fruit juice manufacturing companies in Iran. The samples were tested for the presence of B. licheniformis by culture in specific media and biochemical testing. Suspected samples were also investigated for the presence of the bacterium by polymerase chain reaction using specific primer for the gyrB gene.
Results: Three samples (18.75%) from the 16 tested fruit juice samples were found as positive. In other words, one apple juice sample (25%) and two orange juice samples (16.66%) were contaminated with B. licheniformis.
Conclusion: Isolation of this bacterium indicates the unsuitable manufacturing conditions and ineffective bacterial decontamination, which might also be favorable for the growth of other fruit juice spoilage bacteria.
KEYWORDS: Bacillus licheniformis, Fruit and Vegetable Juices, Polymerase Chain Reaction.
Nasrin Ramezani , Behnaz Vanaky , Nader Shakeri , Zahra Soltanian , Fatemeh Fakhari Rad , Zahra Shams ,
Volume 13, Issue 1 (Jan-Feb 2019)
Abstract
ABSTRACT
Background and Objectives: Diabetes is one of the most common diseases and a major risk factor for cardiovascular disease. Studies have shown that regular exercise can affect apoptosis in cardiomyocytes. The purpose of this study was to investigate the effect of high-intensity interval training on the expression of Bcl-2 and Bax as important apoptosis factors in diabetic rats.
Methods: After inducing diabetes in 20 male Wistar rats (weighing 250 ± 1.15 g), the rats were randomly divided into a control group and a training group. The training group performed high-intensity interval training five days a week for four weeks, and the control group did not perform any training. After the intervention, RNA was extracted and TCF mRNA was subjected to real time RT-PCR for measuring Bax and Bcl-2 expression in the heart tissue of diabetic rats. Data were analyzed by IBM SPSS Statistics V22 using independent t-test. P-values less than 0.05 were considered as statistically significant.
Results: The expression of Bcl-2 increased significantly and the expression of Bax decreased significantly after the four-week training intervention.
Conclusion: The high-intensity interval training can have beneficial effects on the expression of apoptotic genes in rats with type 2 diabetes.
Keywords: Bax, Bcl-2, High intensity interval training, Heart, Type 2 diabetes.
Sepideh Shakeri, Narjes Soltani, Mohammad Reza Javan, Mehrnaz Abdolalian, Hossein Ayatollahi, Seyyede Fatemeh Shams,
Volume 17, Issue 1 (Jan-Feb 2023)
Abstract
Background and objectives: Fanconi anemia (FA) is an autosomal recessive disorder that usually manifest in forms of pancytopenia, hyperpigmentation, and skeletal complications. Mutation in the DNA repair regulatory genes is associated with the development of FA. Examination of chromosomal breakages when chromosomes are exposed to cross-linking agents is a common method of FA diagnosis. This study aimed to evaluate the prevalence and characteristics of patients with FA in Mashhad, northeast of Iran.
Methods: In this study, we evaluated 312 suspected FA patients who had been referred to the laboratory of Ghaem Hospital during 2014-2020. The mitomycin C method was used to identify FA-positive subjects.
Results: After the examinations, 84 patients (26.9%) were cytogenetically positive for FA. Of 84 patients, 48 (57.1%) were male and 36 (42.9%) were female. Thumb abnormality was the most common congenital anomaly (43.2%).
Conclusion: Based on the findings, males are more susceptible to FA, and thumb abnormality is the most common congenital anomaly associated with FA. Combination of clinical manifestations and genetic susceptibility in patients may contribute to a more accurate diagnosis.
