Showing 9 results for Vakili
Nasrollahi Omran A(phd), Vakili L(msc), Jafarpur M(phd),
Volume 5, Issue 1 (spring-summer[PERSIAN] 2011)
Abstract
Abstract Background and objectives: Genital tract infections are among the most common causes of patients referred to therapeutic centers. Nearly 75% of women suffer from genital Candida infection, at least once in their lifetime. The aim of present study was detection of Candida species causing vaginitis and the evaluation of antimycotic effects of ketoconazol, clotrimazole and fluconazole against Candida species. Material and Methods: In this study, 210 vaginal samples were obtained from the patients suspected of Vaginal Candidiasis. Direct examination and culture were carried out for all specimens to detect the yeast. The isolated yeast species were then identified, using various different tests such as culture on corn meal agar, tween-80, germ tube test, and assimilation test by API 20C kit by using Sabouraud Dextrose Agar and microdilution broth, MIC90 and MIC50 of drug were measured and determined their drug resistance. Results: In the present study, 100 yeast colonies were isolated from patients %80 are C. albicans and the rest are C. parapsilosis(2%), C. tropicalis(6%), C. glabrata(4%), C. krusei(2%), C. guilliermondii (3%), C.stellatoidea(3%). In terms of drug resistance test MIC50 and MIC90 of fluconazole for candida albicans are 5.33 and 35.27μg/ ml, respectively, and for non-albicans candida are 3 and 21.4μg/ml, respectively. Clotrimazole MIC for Candida albicans (MIC50, MIC90) 0.97 and 4.9μg/ml, respectively, and for non-albicans 0.63 and 3.4/ml, respectively. Kectoconazole MIC for Candida albicans 2.43 and 16.45μg/ml, respectively, and for non-albicans 1.12 and 6.6μg/ml, respectively. Conclusion: Clotrimazole has been better than the two other drugs for Candida species on the whole, non albicans species are more sensitive than albicans species in the presence of the drugs used in this study. Key words: Candida, vaginal candidiasis, Resistance drug , Tonekabon.
Rezanezhadi, M, Tabarraei, A, Zhand, S, Moradi, A, Nezamzade, R, Vakili, Ma,
Volume 8, Issue 5 (winter[PERSIAN] 2015)
Abstract
Abstract Background and Objective: Lamivudine is the first orally available drug approved for treatment of chronic hepatitis B. Mutations at the YMDD and FLLAQ motifs in the domains of HBV polymerase gene contribute resistance to lamivudine. This study was aimed to determine the rate of YMDD and FLLAQ mutants in hepatitis B patients in Golestan Province, Iran. Material and methods: In this cross sectional study, 120 patients with chronic HBV infection were recruited. Of them, 55 were treated and 65 untreated with Lamivudine. HBV DNA extractions from plasma and polymerase chain reaction (PCR) were performed. For detection of Lamivudine mutants direct sequencing and alignment of products were applied using reference sequence from Gene Bank database. Results: the average age of patients was 36.31±10.07, which 35% of them were female and 65% were male. Mutations at the YMDD and FLLAQ motifs in the domains of HBV polymerase gene were detected in 12 of 55 patients (21.81%) treated with Lamivudine while no mutation was observed in in untreated patients. The YMDD and FLLAQ mutants were detected in 9.16% (11/120) and 0.83% (1/120) of chronic HBV patients, respectively. Conclusion: Usual HBV mutations, which play an important role in lamivudine resistance, detected in this study are similar to other studies. Key words: Hepatitis B Viruse, YMDD Mutation, Lamivudine, Iran.
Abdollahi, E. (bsc), Tavasolian, F. (bsc), Vakili, M. (phd), Samadi, M. (phd),
Volume 9, Issue 1 (March, April[PERSIAN] 2015)
Abstract
Abstract
Background and Objective: C- reactive protein (CRP), as an acute phase reactant and a reliable marker of inflammation, increases due to inflammatory diseases such as Rheumatoid Arteritis and infectious conditions. New evidence shows that Vitamin D may have important effects on adjusting and reducing the Immune Responses. The aim of this study was to evaluate the association between serum vitamin D as an immunomodulator factor and CRP as an inflammatory factor in Arteritis Patients. .
Material and Methods: The CRP and Vitamin D were evaluated in Rheumatoid Arteritis patients confirmed by Medical records (40 men and 40 women) and in 80 healthy adult people with normal CRP and Vitamin D and no history of arteritis (40 men and 40 women). Torbidometry was used to measure CRP and Eliza for Vitamin D.
Results: In patient group, the mean of CRP and Vitamin D were 95.9±9.1 Mlg/lit and 9.17±2.9 Mlg/lit, resoectively. There was a significant inverse correlation between C-reactive protein and vitamin D in Rheumatoid Arteritis patients (Pvalue= 0.03 Pearson correlation: -0.62) and that was the case for healthy people (p value: 0.04 Pearson correlation: -0.73).
Conclusion: Based on the findings, inverse correlation is observed between serum vitamin D and CRP level.
Keywords: Vitamin D, CRP, Rheumatoid Arteritis
M Vakili (phd), N Jomeh Pour, E Zarifi , M Baghbanian , A Dehghan , M Sahimi , L Gudarzi ,
Volume 9, Issue 3 (Jul,Aug2015[PERSIAN] 2015)
Abstract
Abstract
Background and Objective: Given that microbial contamination is the third largest cause of mortality caused blood transfusion, the examination of contamination in platelet concentrates is essential in blood transfusion centers. The purpose of this study was to achieve a rapid test for bacterial contamination of platelets concentration.
Material and Methods: This laboratory study was conducted on 14 bags of platelet concentrates prepared from Yazd Blood Transfusion Center. Six platelet bags were infected by Staphylococcus epidermidis; six by Klebsiella with a concentration of 150, 15 and 1.5, and two bags were considered as control. In specific intervals, the bags were sampled aseptically and examined by the methods including culture, gram stain, Glucose and PH measurement.
Result: Due to the presence of dextrose, the initial glucose level of platelet bags was above 300 mg/dl. The mean of Glucose in contaminated platelet bags was progressively decreased in 3 days in that it reached 165 mg/dl in the third day ( p = 0.002) . The level of PH had a declining process in that it averagely decreased from PH 7.3 to PH 5.2 (P=0.017(. The results of culturing and smear of the bacteria were different according to the concentrations used in the study.
Conclusion: We can detect the contamination of platelet bags by measuring the level of glucose and PH level in the least amount of time.
Keywords: Blood Platelets; Klebsiella; Staphylococcus Epidermidis.
Badeleh, Mt, Mirzaian, M, Babaei, M, Badeleh, M, Derakhshan Pour, F, Mohammadian, S, Vakili, Ma, Charkazi, A,
Volume 9, Issue 3 (Jul,Aug2015[PERSIAN] 2015)
Abstract
Background and Objective: With regard to high prevalence of attention deficit hyperactivity disorder (ADHD) and its being significantly affected by nutritional factors, we aimed to determine the relationship between zinc serum level and ADHD.
Material and Methods: This ex-post- facto (causal comparative research) design study was conducted on 60 children, selected via convenience sampling. Thirty of them were ADHD children diagnosed by a psychiatrist using DSM IV checklist, as a case group, and the rest were healthy ones located in control group. Having their family informed consent, their Zinc level was measured via Colorimetric method.
Results: The results show that Zinc serum level of ADHD children are lower than that of healthy ones. Using t- test, it was indicated that the difference is not significant. Besides, the result of Pearson correlation coefficient showed that there is no significant relation between Zinc and ADHD.
Conclusion: given that the zinc level was lower in ADHD children, the difference was not significant. Therefore, we recommend conducting further research with a larger sample size.
Fataneh Tavasolian , Elham Abdollahi, Morteza Samadi , Mahmood Vakili ,
Volume 9, Issue 4 (sep,Oct 2015 2015)
Abstract
Abstract
Background and Objective: Methamphetamine has strong stimulating effects on various systems of the human body. The aim of this study was to evaluate the hematological and biochemical parameters in methamphetamine addicts and to compare them with healthyindividuals.
Methods: This is a retrospective case-control study that was conducted in 1390-91 in Ibn Sina Hospital in Shiraz, Iran. Measurement of lipids (cholesterol, triglycerides), liver enzymes (alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase), albumin, blood urea nitrogen, creatinine and blood parameters such as platelets and white blood cells(WBCs) count, hemoglobin concentration and hematocrit of 60 individuals addicted to methamphetamine and 60 healthy subjects as a control group was carried out.
Results: Alanine aminotransferase, aspartate amino transferase, alkaline phosphatase, WBCs and platelet count and serum creatinine levels in methamphetamine addicts were significantly higher than the control group(p-value <0.001), while hemoglobin, hematocrit and albumin levels were lower in these patients (p-value <0.001).
Conclusion: The use of methamphetamine increases lipid peroxidation, changes levels of inflammatory markers and increases liver enzymes, which may increase the risk of liver diseases. It also increases WBCs and platelets count as an early sign of inflammatory disease progression, associated with methamphetamine abuse. Decreased hemoglobin and hematocrit can also increase the risk of anemia in these patients. These observations may give us a better understanding about the biological mechanisms associated with the pathology of methamphetamine consumption in Iran and help us prevent and solve the problems arising from this drug.
Keywords: Methamphetamine, Hematological Tests, Biological Markers, Oxidative Stress
Farzane Salarneia , Sare Zhand , Behnaz Khodabakhshi , Alijan Tabarraei , Mohammad Ali Vakili , Naeme Javid , Masoud Bazori , Abdolvahab Moradi ,
Volume 10, Issue 1 (Jan,Feb 2016 2016)
Abstract
Abstract
Background and objective: Hepatitis B virus (HBV) is a DNA virus with high tendency toward hepatic tissue. There are currently about 3 million HBV-infected people and 350 to 400 million chronic carriers of this virus in the world. X protein plays a role in the over-expression of oncogenes, carcinogenicity of liver cells and overlaps with the basal core promoter of the virus. Mutations at specific nucleotides of this region increase viral replication and liver disease progression. The aim of this study was to investigate the frequency of mutations at nucleotides 1762, 1764 and 1766 of HBV X gene in patients with chronic hepatitis B and hepatitis B-related cirrhosis.
Methods: In this study, 102 patients including 68 chronic hepatitis patients and 34 patients with hepatitis B-related cirrhosis were enrolled. After DNA extraction, HBV X gene was amplified and sequenced using Semi Nested-PCR. Obtained gene sequences were compared with the standard sequence of HBV virus X gene available in the gene bank (Okamoto AB033559). Then, the mutations in the gene X of HBV were identified.
Results: Comparison of the standard sequence with sequences obtained from patients showed the presence of A1762T / G1764A mutation in 12 chronic (17.64%) and 13 cirrhotic (38.23%) patients. Also, C1766G / G1764T mutations were found in 8.23% of chronic patients and 17.64% of cirrhotic patients.
Conclusion: A1762T / G1764A mutations in the overlapping region of the basal core promoter with gene X C-terminal may lead to liver disease progression from chronic hepatitis to cirrhosis, by changing the amino acid sequence of the X protein.
Abbass Ghanbari Niaki, Abolfazl Aghababaeian, Hamidreza Joshaghani, Mohammad Ali Vakili, Mehran Hoseini,
Volume 13, Issue 4 (Jul-Aug 2019)
Abstract
ABSTRACT
Background and Objectives: Apolipoprotein O (apoO) is a 198 amino acids protein that exists predominantly in high-density lipoprotein (HDL). It may exert cardioprotective effects via decreasing fat accumulation and increasing removal of cholesterol from macrophages. Although the health benefits of exercise are well documented, no study has yet investigated the effects of various types of training, including resistance training on apoO level. Therefore, we aimed to determine effects of five weeks of circuit resistance training with different intensities on plasma levels of apoO, HDL, total cholesterol (TC) and triglyceride (TG) in young untrained men.
Methods: Forty-five age- and weight-matched healthy untrained men were randomly assigned to a control group (n=10) and four training groups: training at 20% intensity (n=9), training at 40% intensity (n=8), training at 60% intensity (n=7) and training at 80% intensity (n=8). The subjects performed circuit resistance training at 10 stations (30 seconds at each station) with three repetitions, without rest between stations and with 3-minute active rest between the repetitions. The training was carried out for 45 minutes per session, three sessions a week, for five weeks. Venous blood samples were taken 48 hours before the first exercise session and 48 hours after the last training session. Plasma levels of apoO, HDL, TC and TG were measured using commercial kits. Data were analyzed using repeated measures ANOVA and Tukey's post hoc test at significance level of 0.05.
Results: After the training intervention, mean plasma level of TC and TG did not differ significantly between the study groups (P>0.05). Training at 80% of one-repetition maximum (1RM) caused a slight decrease in the apoO concentrations. Moreover, apoO concentration was significantly higher in the 20% 1RM training group compared to other study groups (F=11.599, P<0.002).
Conclusion: Our results indicate that circuit resistance training at 80% of 1RM can decrease HDL-associated apoO level but does not significantly alter other parameters.
KEYWORDS: Circuit resistance training, HDL-O, TC, TG, Young men.
Abolfazl Aghababaeian, Abbass Ghanbari Niaki, Hamidreza Joshaghani, Mohammad Ali Vakili, S.mehran Hoseini,
Volume 13, Issue 5 (Sep-Oct 2019)
Abstract
ABSTRACT
Background and Objectives: Coronary artery disease (CAD) is the leading cause of death worldwide. It is well established that low level of high-density lipoprotein-cholesterol (HDL-C) is a strong and independent risk factor for CAD. Apolipoprotein M (apoM) is a component of HDL, which is involved in pre-β-HDL formation and cholesterol efflux to HDL. It is believed that resistance and aerobic exercise can significantly reduce risk of cardiovascular disease, especially by increasing serum levels of HDL-C. However, little is known about effects of these activities on HDL-apoM levels. The aim of this study was to investigate effects of circuit resistance training at different intensities on HDL-associated apoM levels in young untrained men.
Methods: Forty-five age- and weight-matched healthy untrained men were randomly assigned to a control group (n=10) and four training groups: 20% 1-repetition maximum (1RM) (n=9), 40% 1RM (n=8), 60% 1RM (n=7) and 80% 1RM (n=8). The subjects performed circuit resistance training consisting of barbell bench press, underarm flab, seated barbell curl, triceps exercise with chains, lying leg curl, squats, hyperextension, abs workout, sit-ups and quadriceps workouts (30 seconds each) in three bouts without rest between stations and with active rest (3 minutes) between sets or bouts. The training protocol was carried out for 45 minutes per session, three sessions a week, for five weeks. Venous blood samples were taken 48 hours before the first exercise session and 48 hours after the last training session. After separating plasma, HDL-associated apoM was measured using commercial ELISA kits. SPSS 16 was used for analysis of data using two-way ANOVA and Tukey's post hoc test at significant level of 0.05.
Results: After the training intervention, the exercise groups had higher apoM levels in total HDL and HDL-2 compared to the control group (P>0.05). However, no significant difference in HDL-associated apoM level was observed between the study groups.
Conclusion: The results of this study indicate that various intensities of circuit resistance training can alter HDL-associated apoM levels. The decreased HDL-3-associated apoM level could indicate increased rate of apoM transfer to HDL-2, which could potentially prevent development of atherosclerosis and CAD by enhancing the antioxidant effects of HDL.
Keywords: Circuit Resistance Training, Total HDL-M, HDL3-M, HDL2-M.
