Showing 3 results for matini
Moradi, M, Matini, M, Mohemmi, N, Maghsoud, A, Zahirnia, A, Parsa, F, Fallah, M,
Volume 9, Issue 2 (may,jun 2015[PERSIAN] 2015)
Abstract
Background and Objective: Assessment of alimentary tract helminthes of rodents has a great zoonotic importance. This study aimed at determining the prevalence of helminth infections in rodents in Hamadan.
Material and Methods: a total of 100 rodents trapped from different parts of city were transported to laboratory. After anesthetizing by chloroform, the animals were undergone an operation to isolates the helminthes. The isolates were stained by Carmine and identified at the genus and species levels. Furthermore, age, sex, weight of rodent and size of various organs of body were determined.
Results: Totally, 62% of the rodents were infected to intestinal helminthes. All trapped rodents were Rattus norvegicus. Six species of helminthes, including three Nematode (45%), 3 Cestode (51%) and no Trematode were isolated from rodents. The infection rate for different helminthes was as follow: Hymenolepis nana 21%, H. diminuta 29%, Heterakis spomosa 43%, Strongyloides sp. 1% Trichuris muris 1% and Cysticercus fasciolaris 1%.
Conclusion: in this area, infection rate of alimentary tract helminthes in the Rattus norvegicus, especially zoonotic helminthes, is relatively high, and the rate of Cestodes is higher than those of Nematodes and Trematodes.
Key words: Prevalence, Helminthes, Alimentary tract, Rodents
Mohammad Fallah, Alireza Shiri, Amir Hossein Maghsood, Mohammad Matini,
Volume 15, Issue 3 (May-Jun 2021)
Abstract
Background and objectives: Hydatidosis is an important zoonotic disease with widespread distribution. For unknown reasons, some cysts are unable to produce protoscoleces, and little is known about the mechanisms involved in infertile cyst production. Therefore, characterization of hydatid cyst fluid (HCF) components could help clarify the host-parasite relationship and the fertility process of cyst. The aim of this study was to identify and quantify biochemical components of HCF of fertile and infertile cysts from different hosts.
Methods: A total of 35 HCF samples were obtained from the liver and lung of 16 cattle, 16 sheep and three humans. Fertility of cysts was determined by examination of cysts’ content. Then, total fluid was aspirated aseptically from each cyst. The samples were centrifuged at 10000×g for 15 min at 4°C, and then concentrated and dialyzed against phosphate buffer saline using an Amicon Ultra-15 5000 MWCO centrifugal filter device (Millipore, USA). All biochemical components were quantified by an automatic analyzer.
Results: The value of lactate dehydrogenase, potassium, calcium, cholesterol, glucose, urea and uric acid differed significantly in cysts collected from different hosts (P<0.001). There was also a significant difference in the amount of lactate dehydrogenase, aspartate aminotransferase and cholesterol between fertile and infertile cysts (P<0.039).
Conclusion: Due to the differences in biochemical composition of HCF of different host origin, it is possible that the host plays a crucial role in determining the type of biochemistry in hydatid cyst as well as in hydatid cyst fertility.
Zarifeh Akbari, Dara Dastan, Mohammad Fallah, Amir Hossein Maghsood, Mohammad Matini,
Volume 19, Issue 5 (9-2025)
Abstract
Background and objectives: Trichomonas vaginalis causes human trichomoniasis, a common type of protozoan vaginitis. Due to the increasing incidence of drug-resistant trichomoniasis, new pharmacological research is needed. The aim was investigation of the activity of Ferula pseudalliacea against T. vaginalis, and preliminary phytochemical analysis of its extracts.
Methods: Essential oil and various extracts of F. pseudalliacea roots, including n-hexane, ethyl acetate and methanol, were obtained. Susceptibility testing of the plant products was done on five T. vaginalis isolates by using microtiter plate method. Minimum lethal concentration (MLC) and growth inhibitory percent (GI%) of sub-MLC concentration were reported, after 24 and 48 hours’ exposures. Phytochemical screening of the extracts was done using standard procedure.
Results: Antitrichomonal effect of the plant products depended on time and concentration. The greatest effect was observed in 48-hour exposure. The essential oils and n-hexane extract of F. pseudalliacea demonstrated a remarkable activity with MLC of 250 µg/ml and following by the ethyl acetate (MLC=500 µg/ml) and methanol extract (MLC=1000 µg/ml), with GI% 92.8, 50.6, 85.2, and 42.8, respectively. The bioactive constituents of the extracts were coumarins, terpenoids, steroids, phenols, tannins and glycosides.
Conclusion: The results of this study demonstrated in vitro antitrichomonal properties of F. pseudalliacea. Therefore, further studies are needed to investigate the potential of the antitrichomonal activity of its bioactive constituents.
goums.ac.ir
yahoo.com