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Abstract Background and objectives: HTLV-1 virus belongs to the retrovirus and infection with this virus mostly is seen among people having more than one time blood transfusion. Because of requiring repeated blood transfusions, thalassemic patients are considered to be high risk subjects in this regard. Thus, this study was carried out to indicate the frequency of HTLV-1 infection among the thalassemic patients. Materials and Methods: Blood samples of 181 thalassemic patients referred to Taleghani hospital during nearly two years (2004-2005) were taken. By using ELISA technique, the sera were assessed to determine HTLV antibody. The positive ones subsequently were examined by western Blot (kit, 2.4) to confirm the ELISA positive samples and also to recognize the HTLV type. Results: Of 181 thalassemic patients, 93 (51.4%) were male. The age was between one and twenty five (14.11 ± 6.5). 93.4% (169) were received packed cell only once in a month. 14.9% (27) were HTLV positive by ELISA technique, while just eight out of these 27 were considered to be true positive by Western blot and to be contaminated by type one virus. Of all subjects, 4.4% were positive HTLV1. Furthermore, the contamination with this virus is increased as the patients getting older. Conclusion: The findings indicated that among the thalassemic patients in Gorgan, there are cases with HTLV-1 whose frequency is correlated with the other part of our country. Consequently, further comprehensive studies are required to identify those infected blood donated to minimize the transmission risk of this infection in the society and in particular among the people receiving blood, such as thalassemic patients. Keywords: HTLV-1 antibody, thalassemic patients, ELISA, western Blood, Gorgan Journal

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Abstract Background and Objectives: Chromogenic media are the newest methods applied for rapid detection of pathogenic microorganisms in drinking water and food from 1998-2008. These Specific media contained the compounds acting as a substrate for microbial enzymes and, according to the type of enzyme, produce specific color. The aim of this study was to introduce the chromogenic media as a powerful tool in rapid detection of pathogenic agents in drinking water and food. Material and Methods: In this review article, the published papers about the use of chromogenic media in rapid detection of water and food-born pathogens were investigated. Results: The studies conducted in different countries show that the chromogenic media are very sensitive, specific and with high performance therefore, we can use it to detect the most important pathogenic microorganisms (such as Salmonella spp, E.coli, S.aureus, L.monocytogenes, and Candida spp.) in water and food samples. Conclusion: Because chromogenic media, in comparison with the other rapid detection methods such as PCR and ELISA, are very sensitive and cheaper, it can be applied as an alternative method. Key words: Chromogenic media, Rapid detection, water and food, Microorganism

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Abstract Background and Objectives: one of the endemic foci for Cutaneous Leishmaniasis in Iran is Mashhad in which limited outbreak have recently been reported. The commonly used method for diagnosis is the clinical features confirmed by direct microspic examination and culture or biopsy. We compared these two tests to determine the level of their sensitivity, specificity and positive predictive value. Material and Methods: we performed this comparative-analytic study on 73 patients suspected of having ulcers Leishmaniasis in Mashhad, Iran. Giemsa was staining the smears and the samples cultivated on Di-phasic N.N.N. culture media . Analysis was performed by SPSS version 11.5 and Chi square test. A P- value less than 0.05 were considered as a significant. Results: In 43 cases (58.9%), both the smear and culture are Positive. In 13 cases (17.8%), the smear is negative but the culture Positive. In 17 cases (23.2%), both smear and culture are negative. The two methods are positively correlated (82%). Sensitivity, Specifity, Positive predictive Value and negative predictive value are 76.7%, 100%, 100% and 56.7%., respectively. Conclusion: when the smear is positive, there is no need for culture. However, the opposite is not true. Key words: Cutaneous Leishmaniasis, Laboratory Diagnostic, Direct Microspic Examination, Culture.

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Abstract Background and objectives: Resistance to antimicrobial agent is a world-wide problem and the existed reports are not the same. Aware of antibiotic resistant pattern of microorganisms in each hospital is important for controlling the nosocomial infections. This study was designed to determine the resistant pattern of microorganisms isolated in Yahya nejad hospital, Babol, north of Iran. Material and Methods: In this descriptive study, organisms were isolated during 2006 from urine, blood, endotracheal tube, abscess, fluids (ascites, pleura, and synovia), wound, discharges and bonemarrow of both outpatients and inpatients in our hospital were studied. Their antibiotics resistances were evaluated by Kirby Bauer method. Results: of 3114 culture specimens of admitted patients (female 1732, male 1382), 325 (10.4%) microorganisms were isolated. They were isolated from urine 162(49.8%), blood 115(35.4%), abscess 11(3.4%), wound, 10(3.1%) and discharge10 (3.1%). The most common microorganisms were Escherichia coli 107(33%) and Staphylococcus epidermidis 69(21.3%). Maximum resistance of E.coli is related to tetracycline (83.9%) and ampicillin (75%) and maximum resistance of S.epidermidis to oxacillin (89.2%) and penicillin (82.1%). Conclusion: With regard to high antibiotic resistance (>70%) in this study, it seems that the early beginning and inappropriate dosage of antimicrobial agents may be associated with developing antibiotic resistance. Hence, it is highly recommended to prohibit the unnecessary prescription of antibiotics Key words: Antibiotic resistance, microorganisms, Blood culture, E.coli

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Abstract Background and Objectives: Incidents related to electromagnetic interference with medical devices have been reported over the past decades. It has also been indicated that the microwave radiation emitted from mobile phones interferes with the operation of medical devices therefore, this study aimed at testing the interference by GSM mobile phones with cell counters. Material and Methods: We did this experimental Study on thirty-two heparinized blood samples of 32 healthy individuals Selected randomly. The Cell Counting was Carried out in the presence of Electro magnetic field produced by three Cell phones with different levels of SAR (Low, intermediate and High) and without being in electromagnetic field. Statistical tests were used to analyze the data (p<0.05). Results: Microwave radiation emitted from cell phones, regardless of their SAR, interferes with the proper operation of cell Counter. This interference leads to false Counting. Conclusion: As mobile phones emit microwave radiation in an isotropic manner, keeping mobile phones at a safe distance, 15cm, from medical equipments will be necessary. These observations confirm the need for some restrictions of mobile phone use in hospitals and medical laboratories. Key words: Interference, Cell Counters, Mobile Phone, Microwave Radiation

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Abstract Background and objectives: Infectious diseases are the leading cause of mortality in the world. With the increase of Microbial resistance to chemical antibiotics and low side effects of medicinal herbs, it has become of great importance to use herbs as a source of antimicrobial compounds. Material and Methods: In this study, the extract of Trigonella foenum leaf and seed was prepared using 70% ethanol. Minimum Inhibitory Concentrations (MIC) and antimicrobial Sensitivity were determined using microdilution broth and disk diffusion method, respectively. The Results were compared with Gentamicin, Ciprofloxacin and Fluconasole. The tested strains were: clinical strains of Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia, Enterococcus faecium, Candida albicans and two standard strains of Staphylococcus aureus and Pseudomonas aeruginosa. Results: The least MIC of the leaf extract against the standard strain of S. S. aureus, E. faecium and clinical S. aureus is 64 μg/ml and for seed extract against E. faecium and Standard S. S. aureus is also 64 μg/ml. The largest diameter of inhibitory zone caused by leaf extract on Standard S. S. aureus is 23mm, and for seed against E. faecium is 18 mm. Conclusion: Because of antimicrobial effects of leaf and seed extract of Trigonella foenum, these extracts can be used as anti microbial compounds in pharmaceutical and food industries. Therefore, the isolation, identification and purification of the antimicrobial compounds of the extracts are recommended. Key words: Trigonella foenum, Antimicrobial effects, Minimum inhibitory concentration.

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Abstract Background and objectives: some parasites especially Enterobius vermicularis and helminthes are the leading factor causing appendicitis. We did this study to determine the prevalence of infection with Enterobius vermicularis among excised appendices in Necoii hospital of Qom, Iran (2005-2006). Material and Methods: In this Cross-sectional Retrospective study, we referred to pathology district archive of Necoii hospital of Qom to collect the demographic data of 3420 appendectomy patients including gender, age and infection to Enterobius vermicularis. These patients had been undertaken appendectomy from March 2005 to March 2006. After confirm of infection, all patients demographic data and histopathological results were analyzed by using Chi-square test in SPSS version 11.5 software. Results: of 3420 appendices, 17 cases (0.5%) are infected with Enterobius Vermicularis. Thirteen of them (76.5%) are female and four (23.5%) male. also 15 of infected appendices (88.2%) don’t show any pathological changes and two cases (11.8%) have appendicitis. The highest infection rate is observed in 6-10 (41.1%) and 11-15 (35.3%) age groups. Conclusion: The results show that Enterobius vermicularis does not play a role in causing appendicitis, and its presence in excised appendix can not be a good reason for being the cause of appendicitis. Key words: Appendicitis, Enterobius vermicularis, Qom, Iran

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Abstract Background and objectives: "The comb antibiotic sensitivity test" is a quick, reliable and cost effective method to determine the susceptibility of bacteria to different antibiotics. The purpose of this study is to design a plate that is easy and quick to use, and enable to be interpreted easily without the need for measurement of the margins with a ruler. Material and Methods: First, Clinical and Laboratory Standards Institute data about the maximum growth inhibitory haloes formed with antibiotics and various micro-organisms were statistically examined and determined that the most (99.7%) zone of inhibition growth is formed in the range of 42 mm. Accordingly, the obtained number (42 mm) and conventional plate size (100 mm) used for testing sensitivity were punched into Solid works software which was used to determine the best place of strip and shoulder plates. After that the efficacy this media were examined by determination of MIC Staphylococcus aureus and Klebsiella pneumonia, non-graded and graded plate shoulders were searched and compared. Results: Has been placed two combs MIC in a plate in this method and didn’t create growth inhibitory haloes interferences. Obtained MIC for Nitrofurantoin( 10 µg/ml) , Amikacin(0.5 µg/ml), Gentamicin(1 µg/ml) , and Amoxicillin (0.5 µg/ml) against S.aureus , MIC Nalidicsic Acid, Amikcin, Gentamicin and Nitrofurantoin against K.pneumonia was 0.1, 0.5, 0.5 and 10 µg/ml ,respectively. Conclusion:The comparison between this new innovative method and standard methods (Clinical Laboratory Standards Institute - CLSI) shows that there a marked reduction in the interference of antibiotic therapy and will also reduce time of interpretation. Key words: Plate, Antibiogram Comb, MIC, Antibiotics, Drug resistance.

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Abstract Background and objectives: Pathogens can be transferred via the hands of the personnel not only to themselves but also to their families or to the patient causing infection especially nosocomial infections. Microbial contamination of hand is caused by contact with patients and their environments or usual devices in the workplace. It seems that contamination of computer devices and handsets are more in hospital than official buildings. The aim of this study was to assess and compare the microbial contamination of computer keyboards, mouse and telephone receivers in a hospital department and an official building. Material and Methods: the sterile swab samples obtained from 32 keyboards, 31 computer mouse and 30 telephone receivers in the official building of Mashhad medical university and central laboratory of Imam Reza hospital were cultured on Blood agar and MacConkey agar plates. Results: Out of 64 samples from the official building, we identify 83 microbial germs. The most common ones are gram-positive Bacilli (n =34, 40.95%) and coagulase-negative Staphylococci (n = 32, 32.53%). Of 29 samples of central laboratory, there are 33 microbial germs .The most common of them are grampositive Bacilli (n = 19, 57.57%) and coagulase-negative Staphylococci (n = 7, 21.21%). Overall, microbial contamination of the computer equipment and handsets is not statistically meaningful (P< 0.05). Some germs like diphtheroid are not existed in laboratory, but two cases of Aspergillus are found. Conclusion: The presence of most of the germs on these devices is due to dusting or normal flora transferred via staffs’ hands. Only two of them, coagulase-positive Staphylococci and Aspergillus, should be considered carefully because they may cause serious infections in staff, their families or patients. Key words: contamination , workers’ desk, microorganism, computer keyboard and mouse

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Abstract Background and objectives: Microbial, physical and chemical properties of drinking water, considering type and quantity of the drinking water, are the factors for determining the acceptability of water. The effect of each of the above components on increasing the quality of water or threatening the health of consumers is also considerable. We aimed to investigate the trace amounts of microbial, physical and chemical parameters of drinking water in Gorgan, comparing to national and international standard. Material and Methods: In this cross sectional study, 50 samples were taken, per month, from four pilot stations in urban water network of Gorgan during one year (2009- 2010). We used instant sampling and performed Enumeration of Coliforms in water culture with most probable number (MPN).Samples taken from the stations during the month were investigated in two general categories of device test and heading-meter. All experiments were performed using standard reference method. Results: of 598 water samples 551 (92.2%) have not Coliforms, 47 (7.8%) contain Coliforms and 16 (2.7%) of them have thermo-tolerant Coliforms. Mean water hardness is 413mg/L, base on carbonate calcium. The amount of fluoride about 0.35 mg and the residual chlorine about 0.6 mg is less than national standard amount. Conclusion: The results show that Gorgan water network is, in view of microbial contamination, safe during almost all days. All the tested samples in terms of physical and chemical parameters are in the range of nation and international standards. Hardness of drinking water of Gorgan is one of the highest. Key words: Bacteriological, Physiochemical, Drinking Water, Gorgan.

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Abstract Background and objectives: Helicobacter pylori is a helical gram negative bacterium with polar flagella, discovered by Warren and Marshall in 1983. Helicobacter pylori exist in the stomach mucus tissue of less than 20% of people under 30 years old, but this amount would increase up to 40% and 60% in 60- year- old people. The aim of this study was to compare three methods of culture media, direct slide staining and the urease test for the rapid diagnosis of bacterium in case of peptic or duodenal ulcer. Material and Methods: In This descriptive study, duplicate biopsy specimens were taken from 82 clients referring to four different Hospitals .In endoscopy room of the Hospitals, a rapid urease test were carried out on one of duplicate specimens for the presence or non-presence of Helicobacter pylori. In order to see the Helicobacter pylori in the tissues, three slides using foushin, giemsa, and gram staining were prepared from the second specimens. Then, the specimens were incubated into selective culture media and incubated for 4-6 days in micoraerophilic condition. Results: Of 82 tested specimens 70(85.5%) and 66(80.5%) are identified as Helicobacter pylori by positive urease and culture medium, respectively. The frequency of foushin, giemsa, and gram staining are 67 (81.7%), 66 (80.5%), and 61 (74.4%), respectively. The foushin staining is the best with 100% sensitivity among the other methods. Conclusion: Based on difference between proportions, There is no significant difference between staining methods (foshin, giemsa, gram staining) and culture media in all cases. Key words: Helicobacter pylori, microscopic methods, urease test, culture media, identification

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Abstract Background and objectives: the increasing use of antibiotics, especially the third generation cephalosporins, is an important factor in the spread of antibiotic resistance in bacteria. The main reason for the development of resistance phenotype such as Extended Spectrum Beta Lactamas (ESBL) is the extensive use of broad-spectrum cephalosporins. In phenotypic survey, the Phenotyping confirmatory test and the minimum inhibitory concentration (MIC) are used. In this study, the prevalence of the isolates resistant to third generation cephalosporin (cefotaxime) was determined based on MIC. Material and Methods: form September 2010 to September 2011, 75 isolates of Klebsiella pneumoniae were collected from the infections of inpatients and outpatients, referred to state and private laboratories of Gorgan. For all of the Klebsiella pneumoniae strains, MIC determination using E-test (company Liofilcheme-Italy) was performed. Results: According to the MIC results, 26 samples (34.6%) are resistant to cefotaxime 22 isolates are completely resistant to concentration of 256μg. Conclusion: Because of the importance of risk of becoming ESBL, further studies are needed to clarify the ESBL in the region. Keywords: ESBL, MIC, Klebsiella pneumoniae, Cephalosporin

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Abstract Background and objectives: The presence of pathogenic bacteria and the factors causing food spoilage are the great challenge for public health. Attention to natural additives instead of chemical preservatives resulted in conducting several studies on plant essential oil and extracts. We aimed at evaluating the antibacterial effect of carboxymethyl cellulose coating enriched by Zataria multiflora essential oil and grape seed extract on rainbow trout meat. Material and methods: In this study, two concentrations of Zataria multiflora essential oil (1% and 2%) and two concentrations of grape seed extract (0.5% and 1%) were used both alone and in combination with Carboxymethyl cellulose coating. Antibacterial effect of these treatments was evaluated by enumeration of bacteria in special culture media. Results: The results obtained in this study demonstrate that Zataria multiflora essential oil in combination with grape seed extract significantly can decrease the number of bacteria and delay the spoilage of the samples (p<0.05). Conclusion: Coating enriched by Zataria multiflora and grape seed extract can properly delay the growth of spoilage microorganisms and prolong the shelf life of meat products. Key words: Carboxymethyl cellulose coating, Zataria multiflora essential oil, Grape seed extract, Microbial flora

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Abstract Background and Objective: Cross-transmission of microorganisms by the hands of health care workers is considered as a main transmission route of nosocomial infections. The aim of this study was to investigate the microbial contamination of health-care worker’s hands while going out of hospital. Material and Methods: Wearing the sterile glove with liquid culture, we obtained 100 Samples from the staff’s hands of three departments (clerical department, emergency ward and central laboratory) of Emam Reza hospital. After that, the samples were cultured. Results: Of all personnel, 40% have the habit of washing their hands. Of these, 95 percent wash their hands with water and soap, and 5 percent with alcohol rubs. Of 100 cultured samples, 90 have microorganisms including non-pathogen gram-positive bacillus (29%), coagulase-positive staphylococcus (39%), coagulase-negative staphylococcus (47%), Enterococci (3%), micrococcus (25%) and candida (3%). Contamination in those who had not washed their hands is 62.6% and in those who washed is 37.7% (P=0.04). Conclusion: Hands of health-care workers become progressively contaminated by the potential pathogens during daily activities. To reduce the rate of contamination, it is helpful if we ask staff to wash their hands while going out of hospital. Keywords: Microbial Flora Hospital’s Staff Hand Washing

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Abstract Background and Objective: Carbohydrates are considered as the major source of energy in physical activity. Studies show that consumption of carbohydrate foods before exercise can balance blood glucose and free fatty acids and increase athletes’ performance. In this study , we compared the effect of three kinds of foods with different glycemic indices on blood glucose (BG) and serum free fatty acids (FFA) in cycling ,male athletes. Material and Methods: In this clinical trial, 21 members of national cycling team randomly allocated to three equal groups of glucose (low glycemic index) ، lentil (low glycemic index) and potato (high glycemic index). First, Fasting blood samples (5ml) were obtained to measure BG and FFA . Then the subjects were asked to eat their foods. After 45 mins of rest, they pedaled with maximal oxygen consumption VO2max) for two hours and again their blood samples were taken to compare with the levels of before interventions. Results: Glucose consumption resulted in a significant decrease in FFA level after 2 hours of pedaling (P = 0.01) but no significant change in BG level. Plasma glucose was higher after eating lentil than that of potato (P<0.05), but it was not true for FFA level of both groups. Conclusion: based on the results, the pre-exercise use of low glycemic index (lentil) compared to high glycemic index (potato) can better lead to increased blood glucose during exercise. Keywords: Glycemic Index, Blood Glucose, Serum Free Fatty Acids, Cyclists

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Abstract Background and Objective: Microsporidia is an obligatory intracellular parasite known as an opportunistic infection in immunocompromised patients. Its laboratory diagnosis is relatively difficult and modified trichrome staining is a standard diagnostic method for detection of Microsporidia. The aim of present study was to identify intestinal Microsporidia in kidney- transplanted patients, using modified Trichrome Staining and Calcofluor White Methods. Material and Methods: In 2012, 180 stool specimens were taken from kidney transplanted- patients given immunosuppressive drugs in Hamadan, Iran. To identify Microsporidia we use modified Trichrome Staining and Calcofluor White Methods. Results: The mean duration of kidney transplant and immunosuppressive drug using was 5.5 Years. Only one female patient was positive for Microsporidia. Conclusions: Owing to low frequency of this opportunistic infection among kidney transplanted patients, we can conclude that their hygienic conditions are good enough and they are not exposed with the parasites. Key words:Microsporidia, Modified Trichrome Staining,Kidney Transplant, Calcofluor White, Hamadan

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Abstract Background and Objective: The Isolation of Nocardia species is complex and time-consuming, which is due to rapid growth of adjacent bacteria. Because of the importance of a specific medium with the ability of controlling intrusive microorganisms, this study aimed at comparing three laboratory methods to introduce the reliable isolation technique for Nocardia species. Material and Methods: The soil samples were collected from different regions of Tehran province, Iran, and carefully transferred to the laboratory. The samples were cultured in three different media including Paraffin Baiting,Humic acid vitamin B agar and Paraffin agar, and incubated for 3-4 weeks at 35 °C. Results: Of 110 soil samples, 31 Nocardia isolates (28.18%) were obtained from the media including Paraffin Baiting, (19 17.27%), Humic acid and vitamin B agar (4 3.63%), and Paraffin agar, (8 7.27%). Conclusion: because of high rate of isolation, low cost and the clearance of colonies suspected nocardia, Paraffin Bait technique is more reliable and efficient compared to the other methods. Key words: Nocardia Soil Paraffin Baiting Humic Acid Vitamin B

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Abstract Background and Objective: This study was aimed to determine the extent of bacterial contamination and drug resistance patterns of isolates colonized in colonoscope and endoscope and in relevant personnel. Material and Methods: A total of 107 samples were obtained from staff of endoscopy and colonoscopy units (SEU and SCU) and gastroenterological imaging equipment. For isolation and identification of the bacteria, swab culture method and biochemical identification test were used, respectively. Antimicrobial resistance profiles, multi-drug resistance (MDR) patterns and phenetic relatedness of these isolates were also analyzed according to standard methods. Results: Most frequent pathogenic bacteria among the SEU and gastroenterological imaging related equipments were included S. aureus (20.8 % and 0 %) Enterococcus spp. (0 % and 5.4%) Pseudomonas spp. (0% and 13.5 %), and Clostridium difficile (0% and 12.5%). Analysis of resistance phenotypes showed a high frequency of MDR phenotypes among the SEU (82.1%), and also in endoscopes, colonoscopes, and other equipments (20%, 50% and 100%, respectively). Phylotyping of S. epidermidis isolates showed the role of staff in transmission of resistance strains to medical equipments and also circulation of strains with identical resistance phenotype among the studied samples. Conclusion: High frequency of pathogenic bacteria in colonoscopes, endoscopes and in the staff of endoscopy & colonoscopy units, and also contamination of these instruments with MDR pathogens emphasize the need for proper disinfection of endoscopes and colonoscopes and also instruction of staff in these units. Key words: Bacterial Contamination Endoscope Colonoscope Antimicrobial Resistance Gastrointestinal Disease.

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Abstract Background and Objective: This study was aimed to determine the extent of bacterial contamination and drug resistance patterns of isolates colonized in colonoscope and endoscope and in relevant personnel. Material and Methods: A total of 107 samples were obtained from staff of endoscopy and colonoscopy units (SEU and SCU) and gastroenterological imaging equipment. For isolation and identification of the bacteria, swab culture method and biochemical identification test were used, respectively. Antimicrobial resistance profiles, multi-drug resistance (MDR) patterns and phenetic relatedness of these isolates were also analyzed according to standard methods. Results: Most frequent pathogenic bacteria among the SEU and gastroenterological imaging related equipments were included S. aureus (20.8 % and 0 %) Enterococcus spp. (0 % and 5.4%) Pseudomonas spp. (0% and 13.5 %), and Clostridium difficile (0% and 12.5%). Analysis of resistance phenotypes showed a high frequency of MDR phenotypes among the SEU (82.1%), and also in endoscopes, colonoscopes, and other equipments (20%, 50% and 100%, respectively). Phylotyping of S. epidermidis isolates showed the role of staff in transmission of resistance strains to medical equipments and also circulation of strains with identical resistance phenotype among the studied samples. Conclusion: High frequency of pathogenic bacteria in colonoscopes, endoscopes and in the staff of endoscopy & colonoscopy units, and also contamination of these instruments with MDR pathogens emphasize the need for proper disinfection of endoscopes and colonoscopes and also instruction of staff in these units. Key words: Bacterial Contamination Endoscope Colonoscope Antimicrobial Resistance Gastrointestinal Disease.

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Abstract Background and Objective: Transmission of pathogens by cosmetics is one of the major health complications. Direct contact with contaminated non-standard cosmetics can have irreparable side effects for the consumers. Thus, the evaluation of microbial contamination in cosmetic products is important. The aim of this study was to assess the microbiological contamination of one of frequently used cream. Material and Methods: In the present study, 135 samples of a special moisturizing cream were randomly selected from pharmacies in Tehran. The microbial contamination assessment, sampling and culturing method were based on the protocol (No.3978) of Iranian Institute of Standard and Industrial Research. Results: sixty-two (46%) out of 135 samples were contaminated. The highest and lowest contaminations observed were Pseudomonas aeruginosa and Bacillus, respectively. Conclusion: Due to the high contamination rate of cosmetic creams, we recommend extremely monitoring and controlling these products by health centers. Keywords: Cosmetics, Microbial Contamination, Pseudomonas Aeruginosa