Showing 32 results for Joshaghani
Mojtaba Zare Ebrahimabad , Hanieh Teymoori, Hamidreza Joshaghani,
Volume 13, Issue 5 (Sep-Oct 2019)
Abstract
ABSTRACT
Background and Objectives: Vitamin D is an essential secosteroid that plays a crucial role in the homeostasis of a few mineral elements, particularly calcium. Since vitamin D deficiency and thyroid diseases are two important global health problems, we aimed to investigate a possible relationship of vitamin D and calcium levels with hypothyroidism in an Iranian population.
Methods: This case-control study was conducted on 175 subjects with hypothyroidism (75 males and 100 females) and 175 euthyroid controls (85 males and 90 females) who were referred to a laboratory in Gorgan, Iran. Serum levels of 25-hydroxyvitamin D, calcium, thyroid-stimulating hormone (TSH), free triiodothyronine (free T3) and thyroxine (total T4) were measured in all participants.
Results: Vitamin D and calcium were significantly lower in patients with hypothyroidism (P<0.0001). Free T3 and calcium levels differed significantly among hypothyroid patients based on their vitamin D status (P<0.0001), but vitamin D levels were within sufficient range in all groups. Moreover, there was a positive correlation between free T3 with vitamin D (r= 0.337, P<0.0001) and calcium (r= 0.361, P<0.0001) levels.
Conclusions: Our results suggest that there may be a relationship between decreased vitamin D levels and thyroid function parameters.
Keywords: Vitamin D Deficiency, Hypocalcemia, Hypothyroidism, Thyrotropin, Thyroxine.
Abolfazl Aghababaeian, Abbass Ghanbari Niaki, Hamidreza Joshaghani, Mohammad Ali Vakili, S.mehran Hoseini,
Volume 13, Issue 5 (Sep-Oct 2019)
Abstract
ABSTRACT
Background and Objectives: Coronary artery disease (CAD) is the leading cause of death worldwide. It is well established that low level of high-density lipoprotein-cholesterol (HDL-C) is a strong and independent risk factor for CAD. Apolipoprotein M (apoM) is a component of HDL, which is involved in pre-β-HDL formation and cholesterol efflux to HDL. It is believed that resistance and aerobic exercise can significantly reduce risk of cardiovascular disease, especially by increasing serum levels of HDL-C. However, little is known about effects of these activities on HDL-apoM levels. The aim of this study was to investigate effects of circuit resistance training at different intensities on HDL-associated apoM levels in young untrained men.
Methods: Forty-five age- and weight-matched healthy untrained men were randomly assigned to a control group (n=10) and four training groups: 20% 1-repetition maximum (1RM) (n=9), 40% 1RM (n=8), 60% 1RM (n=7) and 80% 1RM (n=8). The subjects performed circuit resistance training consisting of barbell bench press, underarm flab, seated barbell curl, triceps exercise with chains, lying leg curl, squats, hyperextension, abs workout, sit-ups and quadriceps workouts (30 seconds each) in three bouts without rest between stations and with active rest (3 minutes) between sets or bouts. The training protocol was carried out for 45 minutes per session, three sessions a week, for five weeks. Venous blood samples were taken 48 hours before the first exercise session and 48 hours after the last training session. After separating plasma, HDL-associated apoM was measured using commercial ELISA kits. SPSS 16 was used for analysis of data using two-way ANOVA and Tukey's post hoc test at significant level of 0.05.
Results: After the training intervention, the exercise groups had higher apoM levels in total HDL and HDL-2 compared to the control group (P>0.05). However, no significant difference in HDL-associated apoM level was observed between the study groups.
Conclusion: The results of this study indicate that various intensities of circuit resistance training can alter HDL-associated apoM levels. The decreased HDL-3-associated apoM level could indicate increased rate of apoM transfer to HDL-2, which could potentially prevent development of atherosclerosis and CAD by enhancing the antioxidant effects of HDL.
Keywords: Circuit Resistance Training, Total HDL-M, HDL3-M, HDL2-M.
Ommolbanin Younesian, Samareh Younesian, Sara Hosseinzadeh, Hamid Reza Joshaghani,
Volume 14, Issue 1 (Jan-Feb 2020)
Abstract
ABSTRACT
Esophageal cancer (EC) is one of the most common types of cancer, especially in Asia. Esophageal squamous cell cancer (ESCC) is the most important histological subtype of EC, which accounts for 90% of all EC cases worldwide. ESCC is highly prevalent in Turkey, Iran, Kazakhstan and northern and central parts of China. Selenium is an essential micronutrient that is required for cellular functioning and synthesis of several selenoproteins. It also modulates the antioxidant defense system, cell cycle and apoptosis. This article reviews the most important molecular mechanisms of EC and investigates the association between selenium level and incidence of EC in high-risk areas.
Keywords: Esophageal cancer, selenium, selenoprotein.
Fahimeh Azadi, Masoomeh Rezanezhadi, Hanieh Bagheri, Laith B Alhusseini, Hamid Reza Joshaghani,
Volume 15, Issue 4 (Jul-Aug 2021)
Abstract
Background and objectives: Tuberculosis (TB) is a serious public health problem and a significant diagnostic and therapeutic challenge worldwide. Molecular diagnostic techniques are crucial parts of the World Health Organization’s new tuberculosis control strategy. This study aims to identify Mycobacterium tuberculosis and rifampin resistance in pulmonary and extra-pulmonary clinical specimens using the Gene Xpert MTB/RIF assay.
Methods: The study was carried out on 220 specimens from pulmonary and extra-pulmonary TB patients that were sent to the Kavosh Laboratory in Gorgan (Iran) during 2018-20. The Gene Xpert MTB / RIF method was applied to detect M. tuberculosis and rifampin resistance.
Results: Of 220 specimens, 15 (6.81%) were found to be positive, four (26.6%) of which were related to pulmonary and 11(73.3%) to extra-pulmonary specimens. None of the positive samples was resitant to rifampin according to assay.
Conclusion: Our findings demonstrate that the Gene Xpert MTB/RIF is able to accurately detect M. tuberculosis in pulmonary and extra-pulmonary specimens. The accurate and early diagnosis of TB infection allows timely therapeutic intervention, which is beneficial not only for the patient but also for possible contacts.
Zahra Eslami, Yahya Mohammadnajad Panah Kandi, Alireza Norouzi, Abdorreza Eghbal Moghanlou, Mehdi Sheikh Arabi, Vahideh Kazeminejad, Seyedeh Somayeh Hosseini Alarzi, Aref Saeidi, Hamidreza Joshaghani,
Volume 16, Issue 3 (May-Jun 2022)
Abstract
Background and objectives: Non-alcoholic fatty liver disease (NAFLD) is a chronic liver disease caused by the accumulation of large amounts of fat in the hepatocytes. Given that atorvastatin is effective for treatment of NAFLD, the present study investigated effects of high-fat/fructose diet (HFFD) with atorvastatin on liver enzymes and lipid profile in a NAFLD rat model.
Methods: Thirty-two male Wistar rats were divided into four groups: 1) normal control, 2) HFFD control, 3) HFFD + atorvastatin, and 4) normal + atorvastatin. The groups received HFFD for 15 weeks to induce hepatosteatosis. Atorvastatin was administrated at the dose of 10 mg/kg/day. Lipid profile and liver enzymes were measured after eight weeks of intervention.
Results: Triglyceride, cholesterol, gamma-glutamyl transferase, and aspartate transaminase were significantly reduced in the HFFD + atorvastatin group compared with the HFFD control group. In addition, cholesterol, high-density lipoprotein, alkaline phosphatase, and gamma-glutamyl transferase were significantly increased in the normal + atorvastatin group compared with the normal control group. Low-density lipoprotein increased significantly in the HFFD + atorvastatin group and the normal + atorvastatin group compared with other groups. There was a significant difference in the alanine transaminase levels between the groups taking atorvastatin. In fact, alanine transaminase level was lowest in the normal + atorvastatin group.
Conclusion: Atorvastatin improves the lipid profile and fatty liver and controls liver enzymes. Therefore, it can be used with caution to improve the lipid profile and reduce the complications of NAFLD.
Abdoljalil Eiri, Hami Kaboosi, Farhad Niknejad, Abdollah Ardebili, Hamid Reza Joshaghani,
Volume 16, Issue 4 (Jul-Aug 2022)
Abstract
Background and objectives: Aflatoxin B1 (AFB1) is the most toxic aflatoxin produced by a large number of Aspergillus species. Successful detoxification of this toxin is an important attempt to improve community health. The aim of this study was to evaluate reducing effects of yeasts isolates from kefir and traditional kefir-like fermented beverages on AFB1 in a broth medium.
Methods: Polymerase chain reaction-sequencing was carried out to identify the yeast isolates from kefir and kefir-like beverages. Effects of the isolates on AFB1 adsorption and biotransformation in peptone dexterose broth medium were evaluated by using high performance liquid chromatography.
Results: Saccharomyces cerevisiae and Kluyveromyces marxianus were isolated from kefir and kefir-like beverages and resulted in 46% and 53% AFB1 adsorption, respectively. The isolates 27Y and 2Y caused 7% toxin biotransformation, while 10% toxin biotransformation was achieved by the isolate 18Y.
Conclusion: Our results indicate that the yeast isolates from kefir and traditional kefir-like products can bind to and detoxify AFB1, thereby reducing its harmful effects.
Mana Zakeri, Elham Alimoradi, Effat Seyyedhashemi, Shayan Marhamati, Vahid Tajari, Hamidreza Joshaghani,
Volume 17, Issue 2 (Mar-Apr 2023)
Abstract
Background and objectives: Systemic lupus erythematosus (SLE) is a chronic autoimmune disease, caused by abnormal innate and adaptive immune responses. Anti-nuclear antibodies (ANA) and anti-double stranded DNA (anti-dsDNA) are reliable biomarkers for diagnosing SLE. Here, we aimed to investigate the serum levels of anti-dsDNA and ANA antibodies, their diagnostic utilities, and their relationship with disease activity and clinical/laboratory manifestations in patients with suspected.
Methods: We evaluated the plasma levels of ANA and anti-dsDNA antibodies in all individuals with suspected SLE (n=668) who had been referred to rheumatology clinics in Gorgan, Iran. The level of antibodies as well as C3, C4, and CH50 were determined using commercially available enzyme-linked immunosorbent assay kits.
Results: The mean level of ANA and anti-dsDNA antibodies differed significantly between the ANA-positive and ANA-negative groups (p<0.001). However, there was no significant difference in the mean values of C3 (p=0.233), C4 (p=0.415, and CH50 (p=0.482) between the two groups. Moreover, there was a significant positive correlation between ANA and anti-dsDNA levels (p<0.001, r=0.50).
Conclusion: Our findings indicate that anti-dsDNA levels are higher in ANA-positive individuals, and there may be a positive correlation between ANA and anti-dsDNA levels. It is recommended to evaluate the diagnostic and prognostic values of ANA and anti-dsDNA antibodies in future studies.
Ommolbanin Younesian, Behnaz Khodabakhshi, Sara Hosseinzadeh, Seyedeh Somayeh Hosseini Alarzi, Samareh Younesian, Mojtaba Pourmomen, Mana Zakeri, Ali Hosseini, Professor Hamidreza Joshaghani,
Volume 17, Issue 5 (Sep-Oct 2023)
Abstract
Background: Although public health interventions have slowed the spread of SARS CoV 2 infections, the worldwide pandemic of COVID 19 is progressing. Thus, effective and safe vaccination against SARS CoV 2 is an important tool for controlling the COVID 19 pandemic. Now in the early stages of COVID 19 vaccination, vaccinated individuals are interested in using antibody tests to confirm vaccination success and estimate the time of protection. Here, we assessed anti spike IgG responses in the general population 2 weeks after the second dose of the Sputnik V vaccine.
Methods: This study included blood samples of 67 individuals without a previous SARS CoV 2 infection taken 14 days after the second dose of the Sputnik V vaccine. Anti spike IgG responses were assessed with an enzyme linked immunosorbent assay (ELISA).
Results: Anti spike IgG was detected in 55 (82.1%) of 67 samples 14 days after the second dose of the Sputnik V vaccine. Antibody levels were significantly lower in males than in females, and 9 (75%) of 12 seronegative individuals were males.
Conclusion: Vaccination resulted in detectable anti spike IgG in 82.1% of individuals, and gender may be an important factor in the humoral response.
Masoomeh Rezanezhadi, Fahimeh Azadi, Shayan Marhamati, Hamid Reza Joshaghani,
Volume 17, Issue 6 (Nov-Dec 2023)
Abstract
Background: Herpes simplex viruses (HSV-1 and HSV-2) are a common cause of dermal, oral, and genital infections worldwide. Rapid diagnosis of infected patients will prevent transmission to uninfected individuals. Therefore, this study aimed to detect HSV-1 and HSV-2 by real-time polymerase chain reaction (RT-PCR) in patients from Golestan, Iran.
Methods: In this cross-sectional study, 645 suspected HSV patients hospitalized in the health centers of Golestan Province were selected between 2015 and 2020. Demographic data were collected, and DNA was extracted from the samples. Detection of HSV-1 and HSV-2 was performed by RT-PCR.
Results: Of the 645 samples tested by RT-PCR, 9 samples (1.39 %) were found to be positive for HSV-1/HSV-2, and the rest of the samples (98.6%) were negative. Of the 9 positive samples, 8 cases (88.8%) were HSV-1, of which 7 cases (77.7%) were from the cerebrospinal fluid (CSF) and 1 case (12.5%) from the vagina. HSV-2 was seen only in 1 vaginal specimen (11.1%).
Conclusion: Early diagnosis of these viral infections is very important as it can lead to early treatment and avoid further symptoms and even death. Thus, molecular approaches can provide an option for quicker diagnosis.
Sharabeh Hezarkhani , Khosro Ghojoghi , Sara Hosseinzadeh , Naser Behnampour , Farshid Fathabadi , Zahra Hesari , Hamid Reza Joshaghani ,
Volume 18, Issue 6 (Nov-Dec 2024)
Abstract
Background: Leptin and adiponectin, two members of the adipokine family, play roles in increasing lipid metabolism and inhibiting lipogenesis. Reduced levels of these cytokines are associated with obesity and insulin resistance. This study aimed to determine the serum levels of leptin and adiponectin in type-2 diabetic patients with and without metabolic syndrome compared to a control group.
Methods: Three groups of individuals participated in this study: 47 type-2 diabetic patients with metabolic syndrome (DM+MetS), 25 type-2 diabetic patients without metabolic syndrome (DM-MetS), and 40 individuals with no history of diabetes or metabolic syndrome (Control group). Fasting blood samples were collected, and serum levels of fasting blood sugar, cholesterol, triglycerides, HDL-cholesterol, and LDL-cholesterol were measured using the enzymatic method. Blood pressure, height, and weight were recorded using stadiometers, while leptin and adiponectin levels were determined via enzyme-linked immunosorbent assay.
Results: A significant difference was observed between the DM+MetS group and the DM-MetS group in serum leptin (p = 0.004) and adiponectin (p < 0.001) levels. In patients with type-2 diabetes and metabolic syndrome, serum leptin (p = 0.530) and adiponectin (p < 0.001) levels were lower compared to the control group.
Conclusion: A decrease in the serum levels of key adipokines, such as leptin and adiponectin, in type-2 diabetic patients may serve as a predictor of metabolic syndrome.
Zahra Eslami , Shayan Marhamaty, Seyyed Mehdi Jafari , Mohadese Khorasani , Mehdi Sheikh Arabi , Hamidreza Joshaghani ,
Volume 19, Issue 1 (4-2025)
Abstract
Background: Bivalent minerals function as crucial cofactors that participate in a multitude of metabolic pathways within the organism. Specifically, zinc (Zn) assumes catalytic, structural, and regulatory roles in numerous biological processes. A severe deficiency in Zn can lead to disruptions in nucleic acid and protein synthesis, impaired cellular proliferation, increased apoptosis, and heightened lipid peroxidation of cellular membranes, a phenomenon associated with a reduced lifespan of red blood cells (RBCs). The objective of this study was to investigate the correlations between Zn status and various erythrocyte indices in a cohort of anemic patients, in comparison to a control group.
Methods: A cohort of 563 participants was enrolled in this investigation. Serum Zn concentration was quantified using a BT-3500 autoanalyzer, while hematological indices were determined via a Sysmex KX21N cell counter. Following confirmation of data normality, Spearman's rank correlation coefficient was employed to analyze the relationship between serum Zn levels and RBC indices.
Results: The mean serum Zn concentration was 102.8 ± 17.6 mg/dL. Serum Zn levels exhibited a weak correlation with RBC and hemoglobin (Hb) concentrations in healthy women, as well as a weak correlation with mean corpuscular hemoglobin concentration (MCHC) in anemic men (p < 0.05). Furthermore, the results indicated significantly higher serum Zn levels, RBC, Hb, hematocrite (HCT), and MCHC in men (p < 0.01), while mean corpuscular volume (MCV) was significantly higher in women (p < 0.01). Notably, in individuals with serum Zn levels < 30 mg/dL, MCHC (p < 0.01) and RBC (p < 0.05) were elevated, whereas Hb (p < 0.05), HCT, MCV, and MCH (p < 0.01) were higher than 30.
Conclusion: Considering the potential impact of varying Zn concentrations on erythrocyte indices, including Hb and MCHC, in both healthy and anemic individuals, careful regulation of its dosage is warranted.
Zeinab Siahmargoie, Mohammad Taher Hojjati, Hadi Bazzazi, Khodaberdi Kalavi, Mana Zakeri, Hadi Joshaghani,
Volume 19, Issue 1 (4-2025)
Abstract
Background: Polycythemia Vera (PCV) is a type of myeloproliferative neoplasm (MPN) in which the progenitors of the erythroid lineage become overactive and produce large amounts of red blood cells (RBCs). More than 90% of people with PCV have a Janus kinase 2 (JAK2) gene mutation. In this study, we examined the status of possible JAK2 gene mutations in people with higher-than-normal hemoglobin (Hb) levels that physicians introduced to the laboratory.
Methods: In this descriptive cross-sectional study, JAK2 alleles were tested for possible JAK2 mutations in genomic DNA of 72 cases using a TaqMan-specific probe.
Results: Out of 72 patients, 24 were women (33.3%) and 48 (66.6%) were men, among them 39 (54.2%) were negative and 33 cases (45.5%) were positive for JAK2 mutation. The data also showed that 15 out of 24 female patients (62.5%) had positive JAK2 mutation, while in the male patients, 18 out of 48 (37.5%) were positive for JAK2 mutation.
Conclusion: According to our research, investigation of the Jak2 mutation, especially in women who had Hb levels upper than normal, seems to be necessary.
