Showing 6 results for Arthritis
Morteza Vahed Jabbari , Aliakbar Abolfathi,
Volume 12, Issue 1 (1-2018)
Abstract
ABSTRACT
Background and Objectives: Osteoarthritis (OS) is the most common type of arthritis and joint disease, especially in women. Proinflammatory cytokines, biochemical factors, specially matrix metalloproteinases, and reactive oxygen species play important roles in joint destruction in this disease. Therefore, the present study aimed to evaluate level of inflammatory factors and its relationship with clinical symptoms of OS in female patients.
Methods: The study was performed on female patients with knee OS, referring to healthcare centers of Tabriz University of Medical Sciences. After measuring the weight and height of patients, clinical symptoms such as severity of pain and physical performance were evaluated using the Knee Injury and Osteopaedic Outcome Score questionnaire. Serum levels of IL-1β, TNF-α, and hs-CRP in fasting blood samples were measured using ELISA kits and immunoturbidimetric assays.
Results: There was a significant association between level of IL-1β and score of pain. There was no significant relationship between the clinical symptoms and level of other inflammatory factors.
Conclusion: The results of the present study showed that the increase in inflammatory factors is correlated with severity of pain in OS patients.
Keywords: Osteoarthritis Knee, Female, Inflammatory Markers.
Hadi Bazzazi, Yaghoub Yazdani, Nasser Behnampour, Hadi Hossein-Nataj, Ali Memarian, Mehrdad Aghaei,
Volume 13, Issue 6 (11-2019)
Abstract
ABSTRACT
Background and Objectives: T helper (Th) lymphocytes play a key role in the pathogenesis of autoimmune diseases. As a new subset of lymphocytes, Th9 is thought to be involved in a wide range of disorders including rheumatoid arthritis (RA). In this study, we evaluated frequency of Th9 and Th2 cells and its correlation with disease activity in patients with different stages of RA.
Methods: The frequency of circulating interleukin 9- and/or interleukin 4-producing CD3+CD8-T cells was determined among 41 patients with established RA, 14 patients with very early RA (VERA) and 23 healthy controls by flow cytometry analysis. Then, correlation of cell frequencies with disease activity score 28 (DAS-28) was assessed. Serum levels of interleukin 6 and anti-citrullinated peptide antibodies were measured by enzyme-linked immunosorbent assay.
Results: Frequency of Th9 cells was significantly higher in RA patients compared to healthy controls (P=0.009). Moreover, mean percentage of circulating Th9 cells in patients with inactive VERA was significantly higher than that in those with active disease (P=0.046). In addition, mean percentage of Th9 cells had a negative correlation with the DAS-28 (r=-0.568, P<0.05). There was no significant correlation between the mean serum level of interleukin 6 and percentage of Th2 and Th9 cells (P>0.05).
Conclusion: Our results suggest that Th9 cells may have a potential role in RA initiation. Thus, targeting Th9 cells could be a promising strategy for advanced RA therapies.
Keywords: Rheumatoid arthritis, Th2 cells, Th9 cells.
Samira Oladazimi, Parvin Farzanegi, Mohammad Ali Azarbayejani,
Volume 14, Issue 1 (1-2020)
Abstract
ABSTRACT
Background and objectives: Matrix metalloproteinase-13 (MMP-13) and activin receptor-like kinase 5 (ALK5) are considered as important factors contributing to knee osteoarthritis (OA) pathogenesis. Here, we compared therapeutic effects of mesenchymal stem cells (MSCs), ozone and exercise training alone and combined on expression of MMP-13 and ALK5 in rats with knee OA.
Methods: Knee OA was induced by a surgical method. Rats with OA were then randomly divided into several groups, including model, MSCs, ozone, exercise, MSCs + ozone, MSCs + exercise, ozone + exercise and MSCs + ozone + exercise groups. Expression of MMP-13 and ALK5 genes was evaluated using RT-PCR. Data were analyzed using SPSS software at significance of 0.05.
Results: Expression of MMP-13 and ALK5 differed significantly between the study groups (P<0.0001). Knee OA was significantly associated with overexpression of MMP-13 and ALK5 in the cartilage tissue of rats with knee OA. Combined therapy with MSCs, ozone and exercise significantly decreased the expression of MMP-13 and ALK5 in the cartilage of rats with OA (P<0.001). Although MSCs, ozone and exercise training were effective to mitigate expression of MMP-13 and ALK5 genes, ozone therapy was more effective compared to the other two therapies.
Conclusion: Although ozone, MSCs and exercise training alone could decrease the expression of MMP-13 and ALK5 genes, combined therapy with MSC, ozone and exercise is more effective.
Keywords: Osteoarthritis, O3, MSCs, exercise, MMP-13, ALK5.
Hassan Rasouli, Parvin Farzanegi, Hajar Abbaszadeh,
Volume 14, Issue 6 (11-2020)
Abstract
Background and objectives: Osteoarthritis is one of the most common arthritic diseases and a main cause of pain and disability. Simultaneous downexpression of paired box 7 (Pax7) and myogenin genes, as indicators of satellite cells activation is evident in osteoarthritis. This study assessed effects of an exercise training course and stem cell injection on the expression of Pax7 and myogenin in gastrocnemius muscle of rats with arthritis.
Methods: Thirty five male rats aged 6–8 weeks and weighing 250–300 g were divided into five groups: control, patient, exercise, mesenchymal stem cell (MSC), and exercise+MSC. Osteoarthritis was induced in rats by surgery. The training program consisted of 30 minutes of running on a non-slip treadmill at a speed of 16 m/min. The rats were injected with 1×106 cells/kg MSC. The expression of Pax7 and myogenin was measured by real–time PCR. Data were analysed with SPSS (version 23) using one-way analysis of variance.
Results: Both Pax7 and myogenin were significantly overexpressed in the exercise+MSC group compared to the patient group (P<0.001).
Conclusion: The combination of MSC therapy and training had more positive effects on Pax7 and myogenin expression compared to training and MSC therapy alone.
Hadi Alinejad, Asieh Abbassi Daloii, Parvin Farzanegi, Ahmad Abdi,
Volume 15, Issue 1 (1-2021)
Abstract
Background and Objective: Osteoarthritis (OA) and cardiovascular disease (CVD) are highly prevalent. The purpose of the present study was to investigate the effect of regular aerobic training and hyaluronic acid on cardiac tissue Wnt signaling pathway in experimental model of knee OA.
Methods: 42 male rats were divided into 6 groups (7 in each group): 1) control, 2) patient, 3) salin, 4) HA, 5) exercise, and 6) exercise + HA. In the training groups, the OA model was first induced, followed by 5 days of running on the treadmill for 5 weeks. Hyaluronic acid was injected intra-articularly. After 12 to 14 hours of fasting and 72 hours after the last training session, cardiac tissue sampling was performed for β-catenin and glycogen synthase kinase-3 (GSK-3β) analysis. The expression of the β-catenin and GSK-3β genes in the cardiac tissue was analyzed by RT-PCR. Data analysis was performed using one-way ANOVA if a significant difference was observed by Tukey's post hoc test (P <0.05).
Results: Induction of OA in rats led to a significant increase in β-catenin gene and a significant decrease in cardiac tissue GSK3 gene compared to healthy control group. The results also showed that regular aerobic training, hyaluronic acid injection, and a combination of both treatments reduced the cateninβ gene and increased the cardiac tissue GSK3 gene compared to the rats of OA group.
Conclusion: Regular aerobic training in combination with hyaluronic acid may exert its protective effect by reducing the expression of β-catenin and increasing the expression of cardiac tissue GSK-3β gene ; this may be caused by the heart disease in the model, empirically preventing osteoarthritis.
Babak Biniaz, Hajar Abaszadeh, Parvin Farzanegi,
Volume 15, Issue 2 (3-2021)
Abstract
Background and objectives: Osteoarthritis is the result of a defect in synovial membrane-covered joint tissues. The purpose of this study was to investigate effects of glucosamine sulfate alone and combined with moderate intensity exercise on serum levels of CS 846 epitope and cartilage oligomeric matrix protein (COMP) in a rat model of osteoarthritis.
Methods: In this study, after inducing osteoarthritis in 42 male Wistar rats (weighting 250±300 g, aged 8 to 12 weeks), the rats were randomly divided into five groups: control-healthy, control-patient, patient-exercise, patient-glucosamine and patient-glucosamine-exercise. The training program consisted of 30 minutes of running on a non-slip treadmill at speed of 16 m/min in the first week with progressive overload principle reaching 50 minutes by the eighth week. The glucosamine groups received oral glucosamine sulfate (250 mg/kg/day) once a day for eight consecutive weeks. The serum levels of CS 846 epitope and COMP were measured using commercial ELISA kits. Data were analyzed using one-way ANOVA and Tukey's post hoc test. All statistical analyses were performed in GraghPad prism 8 and at significance level of 0.05.
Results: Combined exercise and glucosamine supplementation caused a significant decrease in the COMP and CS846
levels. This decrease was more profound compared to that of glucosamine and exercise alone.
Conclusion: Overall, the findings of the present study showed that osteoarthritis increases serum COMP and CS 846 levels. In addition, glucosamine supplementation combined with exercise can significantly improve knee osteoarthritis in rats.
