Showing 5 results for Blood Culture
F Sedighian, A Sanee, H Alaouddoulee, M Arshi, Kh Rekabpoor,
Volume 2, Issue 2 (10-2008)
Abstract
Abstract Background and objectives: Resistance to antimicrobial agent is a world-wide problem and the existed reports are not the same. Aware of antibiotic resistant pattern of microorganisms in each hospital is important for controlling the nosocomial infections. This study was designed to determine the resistant pattern of microorganisms isolated in Yahya nejad hospital, Babol, north of Iran. Material and Methods: In this descriptive study, organisms were isolated during 2006 from urine, blood, endotracheal tube, abscess, fluids (ascites, pleura, and synovia), wound, discharges and bonemarrow of both outpatients and inpatients in our hospital were studied. Their antibiotics resistances were evaluated by Kirby Bauer method. Results: of 3114 culture specimens of admitted patients (female 1732, male 1382), 325 (10.4%) microorganisms were isolated. They were isolated from urine 162(49.8%), blood 115(35.4%), abscess 11(3.4%), wound, 10(3.1%) and discharge10 (3.1%). The most common microorganisms were Escherichia coli 107(33%) and Staphylococcus epidermidis 69(21.3%). Maximum resistance of E.coli is related to tetracycline (83.9%) and ampicillin (75%) and maximum resistance of S.epidermidis to oxacillin (89.2%) and penicillin (82.1%). Conclusion: With regard to high antibiotic resistance (>70%) in this study, it seems that the early beginning and inappropriate dosage of antimicrobial agents may be associated with developing antibiotic resistance. Hence, it is highly recommended to prohibit the unnecessary prescription of antibiotics Key words: Antibiotic resistance, microorganisms, Blood culture, E.coli
A Maleki, Sh Ebrahimian,, M Omranii, A Ranjbar, A Mikaeili,
Volume 3, Issue 1 (4-2009)
Abstract
Abstract Background and objectives: Blood culture is a critical part of evaluation of Neonate suspected wath Septicaemia. This phenomenon is one of the most important causes of neonates in Neonatal. Material and Methods: This study was carried out on 1470 somples of neonates suspected with bacteraemia , using reutine microbiologicul technique. The samples wene assessed in hazrate Masoomeh hospilal of Kermansha,Iran. Results and Conclusions: of all samples, 112 (7.62%) ane pasitive. Most of the Positive cultures were obtained after 24 hours of incubation in broth mediu. we Confirmed this result by using diseriminafiue culture media the Isolated bacteria are Coagulase-negative Staphylococus(28.6%),Alfahymolylic Streptococus(0.09%), staphylococus aureus(10.7%),Klebseilla(6.2%),pseudomonas(12.5%),moraxella(0.9%),acin eto bacter(13.4%), Alcalingenes(13.4%),protenos(1.8%) and salmonela (0.9%). Conclusion: the frequency of Coagulase-negative Staphylococus Isolated from neonates Blood culture is more than the other micro organisms. Generally, the frequency of gram negative bacteria is higher than gram Positive. Key words: Septicaemia, Blood culture, Bacteraemia, Neonatal
S Noorbakhsh, A Mirmohamadpoor, A Tabatabaee,
Volume 3, Issue 2 (10-2009)
Abstract
Abstract Background and objectives: The aim of this study was the detection of S.pneumoniae infection by rapid urinary test and blood culture in children with pneumonia in comparison with healthy children. Material and Methods: This case control study was carried out in pediatric ward of Rasoul Akram hospital in Tehran, Iran (2006 - 200٧).Fifty-four Community acquired pneumonia (CAP) and 50 healthy children were selected by simple sampling. The urinary antigen detection test (BINAX NOW co.) was performed on both cases and controls and blood culture was done for the cases suffered from Pneumonia. Results: Pneumococcal antigenuria is detected in 31.5 % of CAP and 6 % of controls groups. There is Significant difference between cases and controls (fisher test CI 95%, P =0.01). None of the children with nonpneumococcal Pneumonia (positive culture) has antigenuria. Conclusion: The possibility of diagnosis of pneumococcal pneumonia in children, by means of blood culture, is low. Thirty-one percent of CAP is due to S.pneumonia, by using the rapid antigenuria test. There fore, we recommend using rapid urinary antigen test, in addition to blood Culture test. Key words: CAP (community-acquired pneumonia) S pneumoniae urinary antigen test (BINAX now), Blood culture, Children
Asghari Estiar, M, Rafi, A, Heidarzadeh, S, Ohadian Moghadam, S, Mahboubi, R, Monadi Sefidan, A, Allafzadeh, J, Nik Khah, H,
Volume 8, Issue 4 (1-2015)
Abstract
Abstract Background and Objective: One of the main causes of increased mortality in cancer patients is bacteremia. On the other hand, antibiotic resistance is the major cause of treatment failure in malignant diseases especially in hematological malignancies. The aim of this study was to diagnose the bacterial strains isolated from blood specimens of cancer patients and to determine their antibiotic susceptibility. Material and Methods: In this cross-sectional study, 0.5 ml of venous blood was taken from 613 cancer patients especially leukemia, and blood cultures and antibiotic susceptibility tests were performed using standard methods. Using disc diffusion method, antibiotic susceptibility was performed with a wide range of antibiotics. Results: Out of 613 cultured specimens, 153 (25%) were found to be positive including 76.47% of gram negative and 23.53% of gram positive bacteria. The most common isolated bacteria were E. coli, coagulase-negative Staphylococci, Klebsiella, Staphylococcus aureus and Pseudomonas aeroginosa, respectively. Conclusion: It seems that Ceftriaxone is the best choice for the treatment of gram negative caused bacteremia and Gentamicin for bacteremia caused by gram positive agents. Given the high level of resistance to the commonly used antibiotics, it seems reasonable to avoid of early and inappropriate use of antibiotics to prevent the development of drug resistant bacteria. Keywords: Cancer, Blood Cultures, Bacteremia, Antibiotic Resistance
Sadaf Khursheed Baba, Abiroo Jan, Mohd Suhail Lone, Dalip K Kakru, Bashir Ahmad Fomda, Gulnaz Bashir, Nadeem Ahmad Bhat,
Volume 17, Issue 3 (5-2023)
Abstract
Background and objectives: Conventional culture and sensitivity methods take around 48 hours to generate antibiotic sensitivity results after a blood culture is flagged as positive by automated systems. However, it is imperative to initiate early targeted antibiotic therapy for effective management of sepsis and to reduce morbidity, mortality, and cost of treatment. This study aimed to evaluate the direct sensitivity test (DST) as a potential tool to obtain quicker antibiotic susceptibility results from positive BacT/ALERT blood culture vials and the VITEK-2 system (the reference method).
Methods: Blood culture bottles flagged as positive by BacT/ALERT were Gram-stained. Cultures with polymicrobial growth were excluded from the study. The isolates were then simultaneously cultured and processed for the DST using the disk diffusion method. Agreements or errors were interpreted according to the Clinical and Laboratory Standards Institute’s guidelines.
Results: Among 76 Gram-positive isolates, we observed 99.2% essential agreement between the DST and AST. The rate of minor and major errors was 4.04% and 1.18%, respectively. Among 75 Gram-negative isolates, we observed 98.99% essential agreement between the DST and AST. The rate of minor and major errors was 4% and 2%, respectively. No very major error was seen in either Gram-negative or -positive isolates.
Conclusions: The DST results are available earlier than the AST results, which can ultimately help in the early initiation of targeted antibiotic therapy.
