H Ansarinia, F Zare, H Hadinedoushan,
Volume 7, Issue 5 (2-2014)
Abstract
Abstract
Background and Objective: In our country, the Wright test routinely is used for diagnosing brucellosis. Because of its low sensitivity, the range of false-negative results is high. Therefore, we aimed at comparing Wright and ELISA in the people suspected brucellosis.
Material and Methods: The results of Wright, 2ME, Coombs Wright tests were compared with Anti-Brucella IgG, Anti-Brucella IgM. Of 1183 subjects referred for Wright test, 148 of them were investigated for Coombs Wright and 228 for 2ME Wright. In addition to Wright test for 32 cases, Brucella IgG and IgM classes were also experimented.
Results: Wright test was negative in 95.4% of cases. Of these negative results, 2.3% were positive for Coombs Wright. Eight-point-five percent of the cases were positive for Coombs Wright test and 4.7% for 2ME Wright test. Sixteen cases were negative for both Wright and ELISA. In 8 cases of Wright-negative, ELISA IgM class was positive and IgG class was negative, and in 4 cases of Wright-negative, ELISA IgM was negative and IgG was positive. About 4 cases of Wright-positive, IgM and IgG antibody classes were positive.
Conclusion: Due to the mismatch between the results of Wright agglutination test and ELISA method and with regard to availability, high sensitivity and determining the type of antibody classes in ELISA, it is focused on ELISA method for brucellosis diagnosis.
Keywords: Brucellosis Wright ELISA
N Khoramabadi, A Mohabati Mobarez, H Aghababa, B Behmanesh, B Tabaraie, F Atyabi,
Volume 8, Issue 5 (1-2015)
Abstract
Abstract Background and Objective: One of the proteins shared in all strains of Brucella is 31 kDa surface protein (BCPS31) that could be an appropriate target for immunization and serological diagnosis. Material and Methods: In the present study, BCSP31 produced as a recombinant protein in pET28a (+) expression system was utilized, using ELISA, to detect trace specific antibody (IgG) in brucellosis patients' serum that was confirmed by culture. We also evaluated cytokine response of peripheral blood lymphocytes to this protein in the cell culture. Results: The results indicated a significant amount of surface protein antibodies (IgG) in the serum of patients with brucellosis. Evaluation of lymphocyte responses to rBCSP31 also showed a significant IL-12 and IFN-γ production in patients’ lymphocyte cultures. Conclusion: These results suggest that BCSP31 can elicit specific humoral and cellular responses during host infection and it can be used in designing immunization and serologic diagnosis systems. Keywords: Brucellosis, 31kDa Cell Surface Protein, Brucella, Cytokine
