Soltan Dallal, Mm, Rahbar, M, Douraghi, M, Rahimi Forooshani, A, Khan Babaei, Gt, Mobarhan, M, Ghasemi, F,
Volume 8, Issue 5 (1-2015)
Abstract
Abstract
Background and Objective: Cystic fibrosis (CF) is an autosomal recessive genetic disease and Pseudomonas aeruginosa is one of the most common bacteria colonized in CF patients. Growing resistance of this bacterium to antibiotics now a day is a challenge of controlling infection in CF patient. In this study colonization of CF patients with Pseudomonas aeruginosa and antibiotic susceptibility pattern of isolated strains were examined.
Material and Methods: From 100 CF patients, during a year, sputum and bronchial swabs were collected. After culturing the samples, some of them were reported as Pseudomonas aeruginosa using biochemical tests. Mucoid strains of Pseudomonas aeruginosa were identified the same as non-producing alginate strains while for catching single pure colony, repeated passage was used. For determining antibiotic resistance of Pseudomonas aeruginosa to some antimicrobial agents Kirby-Bauer method based on CLSI was used.
Results: Of 100 samples, 40 (40%) were positive for Pseudompnas aeruginosa. The prevalence of P. aeruginosa was 23.8, 36.84 and 80% at the age of 1-3, 4-12 and 13, respectively.
Conclusion: Statistically, there is a significant difference between age and contracting with Pseudomonas aeruginosa in that the higher the age the more colonization with Pseudomonas aeruginosa.
Key words: Pseudomonas Aeruginosa, Cystic Fibrosis, Drug Resistance
Roya Rafiee , Fereshte Eftekhar , Seyed Ahmad Tabatabaei , Dariush Minaee-Tehrani ,
Volume 10, Issue 3 (5-2016)
Abstract
ABSTRACT
Background and Objectives: Pseudomonas aeruginosa is the most frequent opportunistic pathogen isolated from the sputum of patients with cystic fibrosis (CF). Resistance to β -lactam antibiotics may arise from over expression of the naturally occurring AmpC cephalosporinases or acquired extended-spectrum β-lactamases (ESBL). The aim of this study was to determine the antibiotic resistance profiles as well as the prevalence of ESBL and AmpC production in clinical isolates of P. aeruginosa from CF patients in Tehran.
Methods: Antibiotic resistance of 50 non-duplicate P. aeruginosa isolates was determined by the disc diffusion method. AmpC β-lactamase production was detected by the antagonism disc test and ESBL production was detected by the phenotypic confirmatory test. The presence of ESBL and AmpC genes was assessed by PCR, followed by sequencing the PCR products.
Results: The antibiotic resistance rates were as follows: 22% to ceftriaxone, 20% to cefotaxime, 10% to imipenem, 8% to carbenicillin and 6% to ticarcillin, 4% each to cefepime, tobramycin, amikacin and aztreonam and 2% to each piperacilin, meropenem and ceftazidime. AmpC production was observed in 47 isolates (94%) and ESBL production was observed in one isolate (2%). PCR results showed that all isolates carried the blaAmpC β-lactamase gene. One multidrug-resistant isolate carried both blaTEM and blaPER-1 genes.
Conclusion: The results showed that despite the low rate of antibiotic resistance in P. aeruginosa CF isolates,the presence of multiple β-lactamases even in one isolate is alarming and can complicate the already difficult treatment of chronic infections in the lungs of CF patients.
