M Alimohammadi, M Rostami, M Jorfi,
Volume 6, Issue 2 (10-2012)
Abstract
Gestational diabetes mellitus (GDM) is the most common metabolic disorder during pregnancy which is associated with 3 to 4 folds of increase in the risk of maternal and fetal morbidity, if it is not diagnosed early (1). Gestational diabetes occurs from 1% to 14%, and 90% of pregnancy-related diabetes has been related to gestational diabetes (2). Glucose challenge test (GCT) and glucose tolerance test (GTT) are recommended by American Diabetes Association (ADA) for screening and diagnosing of gestational diabetes (3).
In this cross-sectional study, the subjects were the pregnant women during 24-28 weeks of gestation referred to Khatam-o-allanbia laboratory of Arak city in 2009. First, we explained the aim of the project and then obtained the written consent. After being fast for at least 8-12 hours, five ml of their venous blood was drown. For GCT, the blood samples were obtained one hour after consumption of 50g of glucose powder. The Individuals whose GCT was equal or higher than 130 mg/dl were introduced for GTT in the future days.
After 8-12 hours of being fast, blood samples were taken to perform GTT. Then after eating 100 grams of glucose powder, their 1, 2 and 3 hour -venous blood samples were drown to perform glucose test, using glucose testing kit of Pars Azmun Company and Mindary BS-300 autoanalyser.
The average age of referred pregnant women is 27.37±10.24 year. Of 417, 403 (96.64%) have normal fasting plasma glucose (≤ 99 mg/dl) and 14 (3.36%) have abnormal fasting plasma glucose (≥ 100 mg/dl). When normal GCT is considered ≥140 mg/dl and ≥130 mg/dl, 357 (85.61%) and 308 (73.56%) have normal GCT and 60 (14.39%) and 109 (26.14%) have abnormal GCT, respectively. The Average of fasting plasma glucose (FPG) and GCT are 83.86 (73-243) and 118.32 (140-351) mg/dl, respectively. In 9 (64.29%) of women with abnormal FPG, the GCT test is also abnormal.
By performing GTT for the women whose GCT was equal or higher than 130 mg/dl, 24 (5.76%) were identified with gestational diabetes and introduced for treatment. If 140 mg/dl was considered as a cutoff point of GCT, four women (0.94%) would falsely be non-diabetic. Based on this study, the prevalence of GDM is 5.74% that is not so high, compared to the range of 4.8 to 7.4% of the country (4). A study conducted by Soheilykhah et al. on 1071 pregnant women with GCT of ≥ 130 mg /dl, 31.9% of the women had abnormal GCT. After doing GTT, 110 patients (10.2%) have been diagnosed with gestational diabetes (2) that is higher than the prevalence of other studies in Iran.
Due to results of this study and other studies, it seems that the GCT is not a reliable test for screening gestational diabetes. On the other hand, if GCT is going to be used, further studies are required to review the current Cutoff point of this test.
Key words: Glucose challenge test (GCT), Fasting plasma glucose (FPG), Gestational diabetes.
Maryam Moradibinabaj , Mohadese Namjoo , Mojgan Nejabat , Hamidreza Joshaghani ,
Volume 10, Issue 1 (1-2016)
Abstract
ABSTRACT
Background and Objective: The association of Triglyceride/High Density Lipoprotein-Cholesterol (TG/HDL-C) ratio with fasting serum insulin, which is an alternative method of insulin resistance (IR) measurement, is well-recognized. Thus, the measurement of TG/HDL-C ratio is useful to determine both IR and dyslipidemia, which itself is a characteristic of individuals with IR. Therefore, this study aimed to investigate the relationship between TG/HDL ratio as an indicator of IR, with different fasting blood glucose levels.
Methods: This case-control study was performed on 343 volunteers with no history of diabetes or use of blood glucose-lowering medications and fasting blood sugar (FBS) levels of less than 126 mg/dl. After sampling, the subjects were divided into three groups based on their FBS level. First group included healthy subjects with FBS of less than 100 mg/dl. Second group consisted of subjects with impaired fasting glucose (IFG) and FBS of 110-100 mg/dl and a third group including those with impaired glucose tolerance (IGT) and FBS of 110-125 mg/dl.
Results: The amount of TG/HDL-C ratio was 3.8 ± 2.8, 4.0 ± 2.1 and 5.4 ± 3.8 for the healthy group, individuals with IFG and IGT, respectively. The TG/HDL index was significantly different among the tested groups with no significant difference between healthy subjects and subjects with IFG. Moreover, there was a statistically significant difference between the IGT and IFG groups with healthy individuals.
Conclusion: Considering the significant increase of the TG/HDL ratio in groups with impaired glucose, using this index can be helpful in evaluation of glycemic disorder.
Seyedeh Tahereh Haeri, Mohammad Ali Azarbayjani, Maghsoud Peeri,
Volume 13, Issue 4 (7-2019)
Abstract
Background and Objectives: Apoptosis is essential for the survival and normal functioning of multicellular organisms, yet any interruption in this process could be detrimental. Increased production of reactive oxygen species and oxidative stress are key factors affecting apoptosis. Our objective was to determine the impact of exercise with and without vitamin D supplementation on expression of FLIP, Fas, and caspase 8 in lung of rats poisoned with H2O2.
Methods: Forty-eight adult male rats were randomly divided into six groups: (C), (H), (HD), (HE), (HDE) and dimethyl sulfoxide. Groups H, HE, HD and HDE received 1 mmol/Kg intraperitoneal injection of H2O2. HE and HDE groups ran on treadmill for eight weeks. Expression of FLIP, Fas and caspase 8 was measured in lung tissues using RT-qPCR. Statistical analysis of data was carried out using SPSS 22 at significance level of 0.05.
Results: Vitamin D supplementation caused a significant decrease in expression of Fas (P=0.014) and caspase 8 (P=0.016) compared to the control group. However, it significantly overexpressed FLIP in the lung tissues compared to the control group (P=0.005). Exercise with and without vitamin D supplementation had no significant effect on the expression of the apoptosis regulatory genes.
Conclusion: Our results show that VD exerts protective effects on lung tissue by regulating apoptotic factors. Aerobic exercise alone and combined with VD has no significant effect on the apoptotic factors. These results indicate that VD supplementation can reduce lung injury under oxidative stress conditions.
