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Showing 2 results for Integron
Antimicrobial Resistance Pattern and Prevalence of Class 1, 2, and 3 Integrons in Clinical Isolates of Klebsiella Pneumoniae in Loghman-E Hakim Hospital, Tehran
Derakhshan, S, Najar Peerayeh, Sh, Fallah, F, Bakhshi, B,
Volume 8, Issue 4 (1-2015)
Abstract

Abstract Background and Objective: Multiple drug resistance has increased in recent years in Klebsiella pneumoniae isolates. The Integrons are mobile genetic elements that carry antibiotics resistance genes. The aim of this study was to determine antibiotic susceptibility and the prevalence of class 1, 2, and 3 integrons in clinical Klebsiella pneumoniae isolated from clinical specimens. Material and Methods: A total of 108 K. pneumoniae isolates were collected between April and December 2011 from different clinical specimens of Loghman hospital in Tehran and identified by biochemical tests. Susceptibility of isolates to 14 antibiotic disks was determined by disk diffusion method. The template DNA was extracted by freeze-thaw method and the presence of class 1, 2, and 3 integrons was investigated by PCR method. Level of resistance to antibiotics in integron-positive and integron-negative isolates was determined. Results: The highest level of resistance was seen for cefotaxime, ceftriaxone, and amoxicillin-clavulanic acid (55.5%). In 79 isolates (73.14%) class 1 integron and in 57 of 79 isolates (72.15%) resistance to at least two classes of drugs were seen. The class 2 and 3 integrons were not detected. Among integron-negative isolates, 8 isolates (27.58%) had resistance to at least one antibiotic. Conclusion: The prevalence of class 1 integron in resistant K. pneumoniae is high therefore, the monitoring of drug resistance and limiting the use of antibiotics are necessary. Keywords: Klebsiella Pneumoniae, Integron, Multi-Drug Resistance


Dissemination of Class 1 Integron among Different Multidrug Resistant Pseudomonas aeruginosa Strains
Zahra Salimizadeh, Seyed Masoud Hashemi Karouei , Farzaneh Hosseini,
Volume 12, Issue 4 (7-2018)
Abstract
ABSTRACT
            Background and objectives: The present study was conducted to detect class 1 integrons and evaluate antibiotic susceptibility patterns among clinical isolates of P. aeruginosa.
            Methods: Sixty clinical samples from blood, tracheal wounds, burns and urinary tract infections were collected from three general hospitals in Tehran, Iran. Culture of specimens was performed on common bacteriological culture media. Bacteria were  identified based on mobility, pigment production, growth at 42 oC, and oxidase and catalase tests. Overall, 21 P.  aeruginosa strains were isolated. Antimicrobial susceptibility of was evaluated via the disk diffusion method (Kirby-Bauer) according to the CLSI guidelines. Presence of the intI1, sul1, aadA2 and aadB gene cassettes was investigated using PCR. The collected data were analyzed using SPSS software (version 21).
            Results: The most effective antimicrobial agents against P. aeruginosa isolates were tetracycline and gentamicin. All P. aeruginosa isolates were multidrug re­sistant. Moreover, the intI1, sul1, aadA2 and aadB genes were found in 90.5%, 90.5%, 47.6% and 19% of the P. aeruginosa isolates, respectively.
            Conclusion: The results indicate that the presence of aadB, aadA2 and sul1 gene cassetes may play an important role in the dissemination of antimicrobial resistance determinants.
          Keywords: Pseu­domonas aeruginosa, integron, multidrug resistance.
ABSTRACT
            Background and objectives: The present study was conducted to detect class 1 integrons and evaluate antibiotic susceptibility patterns among clinical isolates of P. aeruginosa.
            Methods: Sixty clinical samples from blood, tracheal wounds, burns and urinary tract infections were collected from three general hospitals in Tehran, Iran. Culture of specimens was performed on common bacteriological culture media. Bacteria were  identified based on mobility, pigment production, growth at 42 oC, and oxidase and catalase tests. Overall, 21 P.  aeruginosa strains were isolated. Antimicrobial susceptibility of was evaluated via the disk diffusion method (Kirby-Bauer) according to the CLSI guidelines. Presence of the intI1, sul1, aadA2 and aadB gene cassettes was investigated using PCR. The collected data were analyzed using SPSS software (version 21).
            Results: The most effective antimicrobial agents against P. aeruginosa isolates were tetracycline and gentamicin. All P. aeruginosa isolates were multidrug re­sistant. Moreover, the intI1, sul1, aadA2 and aadB genes were found in 90.5%, 90.5%, 47.6% and 19% of the P. aeruginosa isolates, respectively.
            Conclusion: The results indicate that the presence of aadB, aadA2 and sul1 gene cassetes may play an important role in the dissemination of antimicrobial resistance determinants.
          Keywords: Pseu­domonas aeruginosa, integron, multidrug resistance.
ABSTRACT
            Background and objectives: The present study was conducted to detect class 1 integrons and evaluate antibiotic susceptibility patterns among clinical isolates of P. aeruginosa.
            Methods: Sixty clinical samples from blood, tracheal wounds, burns and urinary tract infections were collected from three general hospitals in Tehran, Iran. Culture of specimens was performed on common bacteriological culture media. Bacteria were  identified based on mobility, pigment production, growth at 42 oC, and oxidase and catalase tests. Overall, 21 P.  aeruginosa strains were isolated. Antimicrobial susceptibility of was evaluated via the disk diffusion method (Kirby-Bauer) according to the CLSI guidelines. Presence of the intI1, sul1, aadA2 and aadB gene cassettes was investigated using PCR. The collected data were analyzed using SPSS software (version 21).
            Results: The most effective antimicrobial agents against P. aeruginosa isolates were tetracycline and gentamicin. All P. aeruginosa isolates were multidrug re­sistant. Moreover, the intI1, sul1, aadA2 and aadB genes were found in 90.5%, 90.5%, 47.6% and 19% of the P. aeruginosa isolates, respectively.
            Conclusion: The results indicate that the presence of aadB, aadA2 and sul1 gene cassetes may play an important role in the dissemination of antimicrobial resistance determinants.
          Keywords: Pseu­domonas aeruginosa, integron, multidrug resistance.

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