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Showing 2 results for Maldi-Tof
Comparing MALDI-ToFMass Spectrometry with Molecular and Biochemical Methods in Identifying Enterococcus Faecium and Enterococcus FaecalisIsolated from Clinical Samples
H Samadikafil, M Mohebatimobarez, M Forouzandehmoghadam,
Volume 7, Issue 1 (4-2013)
Abstract
Abstract Background and Objective: Enterococci are Gram-positive members of human gastrointestinal flora,in Dairy products and environment. they have emerged as important causes of opportunistic nosocomial infections in recent years. In this study we aimed to investigat and compare the efficiency of MALDI-TOFmass spectroscopy method through Biochemical and Molecular methods for detecting Enterococcus faecalis and Enterococcus faecium. Materials and Methods:seventhy five clinical samples were collected for biochemical, molecular and mass spectroscopy investigations. Samples were treated with Esculin hydrolysis, Catalase, Pyrrolidonylaminopeptidase, 6.5% NaCl solution, motility, 0.04% Tellurite, L-Arabinose and Sorbitol. Using specific primesallele specific PCR was used.The samples were then analyzed by MALDI-TOF mass spectroscopy and Biotyper 3 software. Results:Enterococcus faecium andEnterococcus faecaliswere detected in thirty and forty two samples, respectively whereas three samples showed both bacterial infections. Using biochemical analysis, two E.faecium isolates were Arabinose negative and one E. faecalis isolates was Telliurite negative. All sampleswere showed correct bands in PCR results but twoof them didn't show clear bands(on agarose gel). In mass spectroscopy analysis all strains were correctly detected and well defined. Conclusion: According to our results, MALDI-TOF mass spectrometry in comparison with Molecular and Biochemical Methodscould be a reliable and accurate method that can easily and quickly identify and differentiate Enterococcus faecium and Enterococcus faecalisin clinical samples. Key words:Enterococcus faecalis, Enterococcus faecium, MALDI-TOFmass spectrometry,PCR
Identification of Candida species isolated from clinical samples by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis
Kavitha Prabhu , Rashmi Theresa Dsouza , Santhosha Devadiga , Beena Antony , Meena Dias ,
Volume 19, Issue 6 (11-2025)
Abstract
Background: Candida species are responsible for substantial morbidity and mortality among susceptible populations. Contemporary epidemiological data indicate a noticeable shift in infection trends away from Candida albicans (C. albicans) toward non-albicans Candida (NAC) species. The primary objectives of this research were therefore to identify the prevalent Candida species and to assess the efficacy of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the identification of these species.
Methods: The standard protocol was employed for processing all routine clinical samples submitted to the bacteriology and mycology departments. Identification of Candida species isolated from diverse clinical specimens was performed using MALDI-TOF MS between September 2022 and November 2023. Subsequently, these findings were recorded in tables and subjected to statistical analysis.
Results: During the study period, a total of 342 Candida species were isolated. Patients diagnosed with candidiasis had a median age of 46.5 years and exhibited a male predominance. NAC species were the most frequently isolated group, with Candida tropicalis (C. tropicalis) (30.40%) as the most common species, followed by Candida parapsilosis (C. parapsilosis) (14.32%). Furthermore, 40 strains of Candida auris (C. auris) were identified over the 15-month duration of the study (11.69%).
Conclusion: The significance of Candida species, encompassing both NAC and novel, emerging drug-resistant strains, necessitates a cautious approach. Compared to conventional and automated systems that rely on biochemical reactions for the speciation of Candida isolates, MALDI-TOF MS offers a potentially advantageous alternative due to its rapid and accurate results.

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