fa تحقيقي Original Paper <div><strong>ABSTRACT </strong><br> <strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Background and Objectives</strong>: Determining the genetic relationship between <em>S. aureus</em> isolates is important for epidemiological surveillance and control of infections caused by this bacterium. The present study was conducted to determine polymorphisms of coagulase gene (<em>coa</em>) among <em>S. aureus</em> isolates from pastry and cheese samples using restriction fragment length polymorphism (RFLP) analysis.<br> <strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Methods: </strong>Overall, 65 <em>S. aureus</em> isolated from pastry (n=45) and cheese (n=20) samples were examined for the <em>coa</em> gene by polymerase chain reaction (PCR). PCR products were digested with <em>Alu</em>I enzyme and the products were assessed using gel electrophoresis.<br> <strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Results</strong>: Except for two isolates, all isolates were positive in <em>coa</em>-PCR and produced four different PCR products, with molecular sizes ranging from 570 to 970 bp. Overall; five distinct RFLP patterns were detected (I-V). Although pattern types I and III were present in isolates from both samples, types I and IV were mainly present in isolates from cheese and pastry samples, respectively.<br> <strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Conclusion</strong>: PCR-RFLP analysis of the <em>coa</em> gene indicates that <em>S</em><em>. aureus </em>isolates from pastry and cheese samples may be originated from different sources. However, as one pattern type was predominant in each group, it can be concluded that majority of the isolates may have the same origin.<br> <strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Keywords:</strong> <em>Staphylococcus aureus</em>, PCR-RFLP, Coagulase, Pastry, Cheese.</div> Staphylococcus aureus, PCR-RFLP, Coagulase, Pastry, Cheese. 12 17 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-497&slc_lang=fa&sid=1 Reza Hakimi Alni 100319475328460014364 100319475328460014364 No Department of Pathobiology, Faculty of Veterinary Sciences, Bu-Ali Sina University, Hamedan, Ira Abdolmajid Mohammadzadeh mohammadzadeh@basu.ac.ir 100319475328460014365 100319475328460014365 Yes Department of Pathobiology, Faculty of Veterinary Sciences, Bu-Ali Sina University, Hamedan, Ira Pezhman Mahmoodi 100319475328460014366 100319475328460014366 No Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran Mohammad Yousef Alikhani 100319475328460014367 100319475328460014367 No Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran