en قارچ شناسی Mycology تحقيقي Original Paper <div><strong>Background and objectives:</strong> Aspergillosis is a widely distributed infectious disease, which is difficult to manage. According to recent studies, the prevalence of resistant <em>Aspergillus fumigatus </em>has increased from 3.3% to 6.6%. Acquired triazole resistance in <em>Aspergillus</em> species is an evolving global health challenge, which has made the control of diseases caused by <em>Aspergillus</em> a concern. This study was performed to investigate prevalence of azole resistance in <em>Aspergillus</em> isolates from environmental samples.<br> <strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Methods:</strong> In this study, 316 soil samples were collected from three hospitals and a university campus in Gorgan (Iran) from July to September 2017. Two grams of each sample were suspended in 5 ml of 0.2M NaCl with 1% Tween 20. Then, 100 &micro;l of the suspension was plated on sabouraud dextrose agar (SDA) supplemented with chloramphenicol, SDA supplemented with chloramphenicol and voriconazole (VOR, 1 mg/L) and SDA supplemented with chloramphenicol and itraconazole (ITC, 4 mg/L). The plates were incubated at 37 &deg;C and examined for growth after 24, 48 and 72 hours.<br> <strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Results:</strong> We detected <em>Aspergillus fumigatus</em>, <em>Aspergillus flavus</em>, <em>Aspergillus niger</em> and <em>Aspergillus nidulans</em> isolates in 187(59.2%), 84(26.6%), 147(46.5%) and 65(20.6%) samples, respectively. We found no VOR resistant isolate. However, 21 (25%) <em>A. flavus </em>and 16 (8.6%) <em>A. fumigatus </em>isolates were intermediate for VOR. In addition, seven (8.3%) <em>A. flavus</em>, 68 (36.4%)<em> A. fumigatus</em>, 41 (27.9%)<em> A. niger</em> and three (4.5%) <em>A. nidulans </em>isolates were resistant to ITC.<br> <strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Conclusion<em>:</em></strong> We were able to detect <em>A.fumigatus</em>, <em>A. flavus</em>, <em>A. niger </em>from all four sampling sites in Gorgan, North of Iran. <em>A. fumigatus</em> is the most prevalent and most resistant isolate in the studied area. History of previous agriculture activity and use of pesticides in the proximity of sampling sites may have affected the rate of ITC resistance.</div> Aspergillus Fumigatus, Aspergillus Flavus, Azole Resistance, Voriconazole, Iran 27 30 http://mlj.goums.ac.ir/browse.php?a_code=A-10-641-1&slc_lang=en&sid=1 Zahra Mirshekar 100319475328460017679 100319475328460017679 No Student Research Committee, Golestan University of Medical Sciences, Gorgan, Iran Nasser Behnampour 100319475328460017680 100319475328460017680 No Faculty of Health, Department of Biostatistics, Golestan University of Medical Sciences, Gorgan, Iran Abolfazl Amini 100319475328460017681 100319475328460017681 No Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran Ghazal Alizad 100319475328460017682 100319475328460017682 No Student Research Committee, Golestan University of Medical Sciences, Gorgan, Iran Ghorban Mohammad kouchaki 100319475328460017683 100319475328460017683 No 2Faculty Member of Surgical Technology Department, Faculty of paramedicine, Golestan University of Medical Sciences, Gorgan, Iran Farhad Niknejad fniknezhad@yahoo.com 100319475328460017684 100319475328460017684 Yes Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran