Tue, Nov 26, 2024
Volume 17, Issue 5 (Sep-Oct 2023)
mljgoums 2023, 17(5): 16-19 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Shajeie A, Rad M, Askari M, Sharifi K, Hashemi tabar G. Plasmid-Mediated colistin resistance in Escherichia coli isolated from neonatal dairy calves without prior consumption of colistin: A threat to public health. mljgoums 2023; 17 (5) :16-19
URL: http://mlj.goums.ac.ir/article-1-1460-en.html
URL: http://mlj.goums.ac.ir/article-1-1460-en.html
1- Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad
2- Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad
3- Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad ,hashemita@gmail.com
2- Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad
3- Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad ,
Abstract: (1245 Views)
Background: Colistin is the most significant last-line antibiotic for the treatment of multidrug-resistant infections caused by Gram-negative bacteria, especially the Enterobacteriaceae family. The emergence and rapid spread of the plasmid-mediated resistance gene, mcr-1 (mobilized colistin resistance), in some isolates of Escherichia coli in recent years provoked public health concerns since it has been shown that mcr-1 with other resistance genes, such as ESBLs (extended-spectrum beta-lactamases) and carbapenemases, could be carried on a single plasmid concurrently. The excessive consumption of colistin, particularly in the livestock industry, and the transmission of these resistant bacteria from livestock to humans may potentially increase the risk of the spread of resistance in humans. Therefore, this study aimed to detect the prevalence of mcr and carbapenem resistance genes among neonatal calves in Mashhad, Razavi Khorasan Province, Iran.
Methods: In the current study, 200 fecal samples from healthy and diarrheic neonatal calves (≤35 days old) were collected in Mashhad (190 E. coli strains were isolated). Antibiotic susceptibility to ceftazidime, cefepime, cefixime, meropenem, colistin, and ciprofloxacin was examined. The double-disk diffusion method (ceftazidime + ceftazidime/clavulanic acid) was performed on Mueller-Hinton agar (MHA) media to phenotypically distinguish the ESBL producers. Afterward, the Multiplex polymerase chain reaction (PCR) method was used to detect colistin resistance genes (mcr-1, mcr-2, mcr-3, mcr-4, and mcr5), NDM-1 (New Delhi metallo-beta-lactamase 1), and OXA-48 as carbapenemases.
Results: The results of the resistance rate to antibiotics were cefepime, ceftazidime, cefixime, meropenem, and colistin. Based on the findings, 33.7% were phenotypically ESBL producers, 4.21% harbored mcr-1, and no NDM-1 or OXA-48 was detected. Among the mcr-1-positive isolates, 5 strains showed the ESBL phenotype.
Conclusion: The results highlight the need for continued monitoring of antibiotic resistance in livestock and the potential for transmission to humans. The findings also underscore the importance of responsible antibiotic use in both human and animal health to mitigate the spread of antibiotic resistance.
Methods: In the current study, 200 fecal samples from healthy and diarrheic neonatal calves (≤35 days old) were collected in Mashhad (190 E. coli strains were isolated). Antibiotic susceptibility to ceftazidime, cefepime, cefixime, meropenem, colistin, and ciprofloxacin was examined. The double-disk diffusion method (ceftazidime + ceftazidime/clavulanic acid) was performed on Mueller-Hinton agar (MHA) media to phenotypically distinguish the ESBL producers. Afterward, the Multiplex polymerase chain reaction (PCR) method was used to detect colistin resistance genes (mcr-1, mcr-2, mcr-3, mcr-4, and mcr5), NDM-1 (New Delhi metallo-beta-lactamase 1), and OXA-48 as carbapenemases.
Results: The results of the resistance rate to antibiotics were cefepime, ceftazidime, cefixime, meropenem, and colistin. Based on the findings, 33.7% were phenotypically ESBL producers, 4.21% harbored mcr-1, and no NDM-1 or OXA-48 was detected. Among the mcr-1-positive isolates, 5 strains showed the ESBL phenotype.
Conclusion: The results highlight the need for continued monitoring of antibiotic resistance in livestock and the potential for transmission to humans. The findings also underscore the importance of responsible antibiotic use in both human and animal health to mitigate the spread of antibiotic resistance.
Keywords: Drug resistance, Multiple, Escherichia Coli, Colistin, mcr-1 protein, bla NDM-1beta-lactamase, beta-lactamase OXA-48, Livestock
Research Article: Research Article |
Subject:
bacteriology
Received: 2021/11/11 | Accepted: 2022/03/13 | Published: 2024/01/15 | ePublished: 2024/01/15
Received: 2021/11/11 | Accepted: 2022/03/13 | Published: 2024/01/15 | ePublished: 2024/01/15
References
1. Kadri SS. Key Takeaways From the US CDC's 2019 Antibiotic Resistance Threats Report for Frontline Providers. Crit Care Med. 2020;48(7):939-45. [View at Publisher] [DOI] [PMID] [Google Scholar]
2. Reardon S. WHO warns against 'post-antibiotic' era. Nature. 2014. [View at Publisher] [DOI] [Google Scholar]
3. Tanner WD, VanDerslice JA, Goel RK, Leecaster MK, Fisher MA, Olstadt J, et al. Multi-state study of Enterobacteriaceae harboring extended-spectrum beta-lactamase and carbapenemase genes in US drinking water. Sci Rep. 2019;9(1):3938. [View at Publisher] [DOI] [PMID] [Google Scholar]
4. Mills MC, Lee J. The threat of carbapenem-resistant bacteria in the environment: Evidence of widespread contamination of reservoirs at a global scale. Environ Pollut. 2019;255(pt1):113143. [View at Publisher] [DOI] [PMID] [Google Scholar]
5. Walsh TR, Weeks J, Livermore DM, Toleman MA. Dissemination of NDM-1 positive bacteria in the New Delhi environment and its implications for human health: an environmental point prevalence study. Lancet Infect Dis. 2011;11(5):355-62. [View at Publisher] [DOI] [PMID] [Google Scholar]
6. Loqman S, Soraa N, Diene SM, Rolain JM. Dissemination of Carbapenemases (OXA-48, NDM and VIM) Producing Enterobacteriaceae Isolated from the Mohamed VI University Hospital in Marrakech, Morocco. Antibiotics (Basel). 2021;10(5):492. [View at Publisher] [DOI] [PMID] [Google Scholar]
7. Ceccarelli D, Hesp A, Van Der Goot J, Joosten P, Sarrazin S, Wagenaar JA, et al. Antimicrobial resistance prevalence in commensal Escherichia coli from broilers, fattening turkeys, fattening pigs and veal calves in European countries and association with antimicrobial usage at country level. J Med Microbiol. 2020; 69(4): 537-47. [View at Publisher] [DOI] [PMID] [Google Scholar]
8. Kapoor G, Saigal S, Elongavan A. Action and resistance mechanisms of antibiotics: A guide for clinicians. J Anaesthesiol Clin Pharmacol. 2017;33(3):300-5. [View at Publisher] [DOI] [PMID] [Google Scholar]
9. Kelly AM, Mathema B, Larson EL. Carbapenem-resistant Enterobacteriaceae in the community: a scoping review. Int J Antimicrob Agents. 2017;50(2):127-34. [View at Publisher] [DOI] [PMID] [Google Scholar]
10. Mohanty S, Mahapatra A. In vitro activity of tigecycline against multidrug-resistant Enterobacteriaceae isolates from skin and soft tissue infections. Ann Med Surg (Lond). 2021;62:228-30. [View at Publisher] [DOI] [PMID] [Google Scholar]
11. Ilbeigi K, Badouei MA, Vaezi H, Zaheri H, Aghasharif S, Kafshdouzan K. Molecular survey of mcr1 and mcr2 plasmid mediated colistin resistance genes in Escherichia coli isolates of animal origin in Iran. BMC Res Notes. 2021;14(1):107. [View at Publisher] [DOI] [PMID] [Google Scholar]
12. Pecora ND, Li N, Allard M, Li C, Albano E, Delaney M, et al. Genomically informed surveillance for carbapenem-resistant Enterobacteriaceae in a health care system. mBio. 2015;6(4):e01030. [View at Publisher] [DOI] [PMID] [Google Scholar]
13. García-Meniño I, Díaz-Jiménez D, García V, de Toro M, Flament-Simon SC, Blanco J, et al. Genomic characterization of prevalent mcr-1, mcr-4, and mcr-5 Escherichia coli within swine enteric colibacillosis in Spain. Front Microbiol. 2019;10:2469. [View at Publisher] [DOI] [PMID] [Google Scholar]
14. Touati A, Mairi AJMDR. Plasmid-determined colistin resistance in the North African countries: A systematic review. Microb Drug Resist. 2021;27(1):121-33. [View at Publisher] [DOI] [PMID] [Google Scholar]
15. Liu YY, Wang Y, Walsh TR, Yi LX, Zhang R, Spencer J, et al. Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study. Lancet Infect Dis. 2016;16(2):161-8. [View at Publisher] [DOI] [PMID] [Google Scholar]
16. Janssen AB, Schaik W. Harder, better, faster, stronger: Colistin resistance mechanisms in Escherichia coli. PLoS Genet. 2021;17(1):e1009262. [View at Publisher] [DOI] [PMID] [Google Scholar]
17. Knopp M, Babina AM, Gudmundsdóttir JS, Douglass MV, Trent MS, Andersson DI. A novel type of colistin resistance genes selected from random sequence space. PLoS Genet. 2021;17(1):e1009227. [View at Publisher] [DOI] [PMID] [Google Scholar]
18. Nguyen HT, Venter H, Veltman T, Williams R, O'Donovan LA, Russell CC, et al. In vitro synergistic activity of NCL195 in combination with colistin against Gram-negative bacterial pathogens. Int J Antimicrob Agents. 2021;57(5):106323. [View at Publisher] [DOI] [PMID] [Google Scholar]
19. Snyman Y, Whitelaw AC, Reuter S, Maloba MRB, Newton-Foot M. Colistin Resistance Mechanisms in Clinical Escherichia coli and Klebsiella spp. Isolates from the Western Cape of South Africa. Microb Drug Resist. 2021;27(9):1249-58. [View at Publisher] [DOI] [PMID] [Google Scholar]
20. Rega M, Carmosino I, Bonilauri P, Frascolla V, Vismarra A, Bacci C. Prevalence of ESβL, AmpC and Colistin-Resistant E. coli in Meat: A Comparison between Pork and Wild Boar. Microorganisms. 2021;9(2):214. [View at Publisher] [Google Scholar] [DOI] [PMID] [View at Publisher] [DOI] [PMID] [Google Scholar]
21. Huang P-H, Cheng Y-H, Chen W-Y, Juan C-H, Chou S-H, Wang J-T, et al. Risk factors and mechanisms of in vivo emergence of colistin resistance in carbapenem-resistant Klebsiella pneumoniae. Int J Antimicrob Agents. 2021;57(6):106342. [View at Publisher] [DOI] [PMID] [Google Scholar]
22. Girlich D, Bogaerts P, Bouchahrouf W, Bernabeu S, Langlois I, Begasse C, et al. Evaluation of the Novodiag CarbaR+, a novel integrated sample to result platform for the multiplex qualitative detection of carbapenem and colistin resistance markers. Microb Drug Resist. 2021;27(2):170-8. [View at Publisher] [DOI] [PMID] [Google Scholar]
23. ECDC, EFSA Panel on Biological Hazards (BIOHAZ); EMA Committee for Medicinal Products for Veterinary Use (CVMP) . ECDC, EFSA and EMA Joint Scientific Opinion on a list of outcome indicators as regards surveillance of antimicrobial resistance and antimicrobial consumption in humans and food‐producing animals. EFSA J. 2017;15(10): e05017. [View at Publisher] [DOI] [PMID] [Google Scholar]
24. Nadimpalli ML, Stewart JR, Pierce E, Pisanic N, Love DC, Hall D, et al. Face mask use and persistence of livestock-associated Staphylococcus aureus nasal carriage among industrial hog operation workers and household contacts, USA. Environ Health Perspect. 2018;126(12):127005. [View at Publisher] [DOI] [PMID] [Google Scholar]
25. Nadimpalli M, Stewart JR, Pierce E, Pisanic N, Love DC, Hall D, et al. Livestock-associated, antibiotic-resistant Staphylococcus aureus nasal carriage and recent skin and soft tissue infection among industrial hog operation workers. PloS one. 2016;11(11):e0165713. [View at Publisher] [DOI] [PMID] [Google Scholar]
26. Abbasian H, Hajimolaali M, Yektadoost A, Zartab S. Antibiotic utilization in Iran 2000-2016: pattern analysis and benchmarking with organization for economic co-operation and development countries. J Res Pharm Pract. 2019;8(3):162-7. [View at Publisher] [DOI] [PMID] [Google Scholar]
27. Tiseo K, Huber L, Gilbert M, Robinson TP, Van Boeckel TP. Global Trends in Antimicrobial Use in Food Animals from 2017 to 2030. Antibiotics (Basel). 2020;9(12):918. [View at Publisher] [DOI] [PMID] [Google Scholar]
28. Markey B, Leonard F, Archambault M, Cullinane A, Maguire D. Clinical veterinary microbiology. Sec ed. Edinburgh: Elsevier Health Sciences; 2013. [View at Publisher] [Google Scholar]
29. Moore E, Arnscheidt A, Krüger A, Strömpl C, Mau M. Simplified protocols for the preparation of genomic DNA from bacterial cultures. Molecular Microbial Ecology Manual. 1999;1(1):1-15. [View at Publisher] [Google Scholar]
30. Rebelo AR, Bortolaia V, Kjeldgaard JS, Pedersen SK, Leekitcharoenphon P, Hansen IM, et al. Multiplex PCR for detection of plasmid-mediated colistin resistance determinants, mcr-1, mcr-2, mcr-3, mcr-4 and mcr-5 for surveillance purposes. Euro Surveill. 2018;23(6):17-00672. [View at Publisher] [DOI] [PMID] [Google Scholar]
31. Borowiak M, Fischer J, Hammerl JA, Hendriksen RS, Szabo I, Malorny B. Identification of a novel transposon-associated phosphoethanolamine transferase gene, mcr-5, conferring colistin resistance in d-tartrate fermenting Salmonella enterica subsp. enterica serovar Paratyphi B. J Antimicrob Chemother. 2017;72(12):3317-24. [View at Publisher] [DOI] [PMID] [Google Scholar]
32. Chen Y, Zhou Z, Jiang Y, Yu Y. Emergence of NDM-1-producing Acinetobacter baumannii in China. J Antimicrob Chemother. 2011;66(6):1255-9. [View at Publisher] [DOI] [PMID] [Google Scholar]
33. Poirel L, Héritier C, Tolün V, Nordmann P. Emergence of oxacillinase-mediated resistance to imipenem in Klebsiella pneumoniae. Antimicrob Agents Chemother. 2004;48(1):15-22. [View at Publisher] [Google Scholar] [DOI] [PMID] [View at Publisher] [DOI] [PMID] [Google Scholar]
34. Vidovic N, Vidovic S. Antimicrobial resistance and food animals: Influence of livestock environment on the emergence and dissemination of antimicrobial resistance. Antibiotics (Basel). 2020;9(2):52. [View at Publisher] [DOI] [PMID] [Google Scholar]
35. Laconi A, Mughini-Gras L, Tolosi R, Grilli G, Trocino A, Carraro L, et al. Microbial community composition and antimicrobial resistance in agricultural soils fertilized with livestock manure from conventional farming in Northern Italy. Sci Total Environ. 2021;760:143404. [View at Publisher] [DOI] [PMID] [Google Scholar]
36. Kampouris ID, Agrawal S, Orschler L, Cacace D, Kunze S, Berendonk TU, et al. Antibiotic resistance gene load and irrigation intensity determine the impact of wastewater irrigation on antimicrobial resistance in the soil microbiome. Water Res. 2021;193:116818. [View at Publisher] [DOI] [PMID] [Google Scholar]
37. Chee-Sanford JC, Aminov RI, Krapac IJ, Garrigues-Jeanjean N, Mackie RI. Occurrence and diversity of tetracycline resistance genes in lagoons and groundwater underlying two swine production facilities. Appl Environ Microbiol. 2001;67(4):1494-502. [View at Publisher] [DOI] [PMID] [Google Scholar]
38. Kumar K, Gupta SC, Chander Y, Singh AK. Antibiotic use in agriculture and its impact on the terrestrial environment. Advances in agronomy. 2005;87:1-54. [View at Publisher] [DOI] [Google Scholar]
39. Marshall BM, Levy SB. Food animals and antimicrobials: impacts on human health. Clin Microbiol Rev. 2011; 24(4): 718-33. [View at Publisher] [DOI] [PMID] [Google Scholar]
40. Aalipour F, Mirlohi M, Jalali M. Determination of antibiotic consumption index for animal originated foods produced in animal husbandry in Iran, 2010. J Environ Health Sci Eng. 2014; 12(1): 42. [View at Publisher] [DOI] [PMID] [Google Scholar]
41. Pishnian Z, Haeili M, Feizi A. Prevalence and molecular determinants of colistin resistance among commensal Enterobacteriaceae isolated from poultry in northwest of Iran. Gut pathog. 2019;11(1):1-8. [View at Publisher] [DOI] [PMID] [Google Scholar]
42. Nikkhahi F, Robatjazi S, Niazadeh M, Javadi A, Shahbazi G, Aris P, et al. First detection of mobilized colistin resistance mcr-1 gene in Escherichia coli isolated from livestock and sewage in Iran. New Microbes New Infect. 2021;41:100862. [View at Publisher] [DOI] [PMID] [Google Scholar]
43. Filioussis G, Kachrimanidou M, Christodoulopoulos G, Kyritsi M, Hadjichristodoulou C, Adamopoulou M, et al. Short communication: Bovine mastitis caused by a multidrug-resistant, mcr-1-positive (colistin-resistant), extended-spectrum β-lactamase-producing Escherichia coli clone on a Greek dairy farm. J Dairy Sci. 2020;103(1):852-7. [View at Publisher] [DOI] [PMID] [Google Scholar]
44. Zhang X, Zhang B, Guo Y, Wang J, Zhao P, Liu J, et al. Colistin resistance prevalence in Escherichia coli from domestic animals in intensive breeding farms of Jiangsu Province. Int J Food Microbiol. 2019;291:87-90. [View at Publisher] [DOI] [PMID] [Google Scholar]
45. Ngbede EO, Poudel A, Kalalah A, Yang Y, Adekanmbi F, Adikwu AA, et al. Identification of mobile colistin resistance genes (mcr-1.1, mcr-5 and mcr-8.1) in Enterobacteriaceae and Alcaligenes faecalis of human and animal origin, Nigeria. Int J Antimicrob Agents. 2020;56(3):106108. [View at Publisher] [DOI] [PMID] [Google Scholar]
46. Gao Y, Lu C, Shen D, Liu J, Ma Z, Yang B, et al. Elimination of the risks of colistin resistance gene (mcr-1) in livestock manure during composting. Environ Int. 2019;126:61-8. [View at Publisher] [DOI] [PMID] [Google Scholar]
47. Koutsianos D, Athanasiou LV, Dimitriou T, Nikolaidis M, Tsadila C, Amoutzias G, et al. Antibiotic resistance patterns and mcr-1 detection in avian pathogenic Escherichia coli isolates from commercial layer and layer breeder flocks demonstrating colibacillosis in Greece. Microb Drug Resist. 2021;27(5):710-20. [View at Publisher] [Google Scholar] [DOI] [PMID] [View at Publisher] [DOI] [PMID] [Google Scholar]
48. Authority EFS. The European :union: Summary Report on Antimicrobial Resistance in zoonotic and indicator bacteria from humans, animals and food in 2017/2018. EFSA J. 2020;18(3):e06007. [View at Publisher] [DOI] [PMID] [Google Scholar]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Enamad
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.