The recognizing of fli C gene in Pseudomonas aeruginosa isolated from clinical sample with PCR

Sattari, M; Imani fooladi, AA; Godarzi, GH

Volume 3, Issue 1 (Spring - Summer 2009[PERSIAN] 2009) mljgoums 2009, 3(1): 0-0 | Back to browse issues page

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Sattari M, Imani fooladi A, Godarzi G. The recognizing of fli C gene in Pseudomonas aeruginosa isolated from clinical sample with PCR. mljgoums 2009; 3 (1)
URL: http://mlj.goums.ac.ir/article-1-26-en.html

The recognizing of fli C gene in Pseudomonas aeruginosa isolated from clinical sample with PCR

M Sattari

¹, AA Imani fooladi

, GH Godarzi

1- , Sattarim@modares.ac.ir

Abstract: (34841 Views)

Abstract Background and objectives: Pseudomonas aeruginosa as an opportunistic pathogen can establish lethal infections in immunocompromised patients or those exposed to predisposing factors. This bacterium contains a single polar flagellum causing motility, chemotaxis and colonization in acute phase of infection. The flagella filament is made up of a structural protein called flagellin. This study was aimed at determining The frequency of fliC gene in Clinical Samples. Material and Methods: In this study, a pair of specific primer for types of flagellin (a, b type) was designed and by using PCR method its structural gene (fliC) was recognized and amplified in clinical strains. Results: This original primer has appropriate efficiency in diagnostic of pseudomonas aeroginosa flagellum. Our study shows that 85% of the Clinical Samples have a fliC gene. Conclusion: This method can be applied to recognizing of the motile strains, and their antigenic typing, and complete amplification of fliC sequence in order to cloning and expression of recombinant flagellin. Key words: Pseudomonas aeruginosa, flagellin, fliC, PCR

Keywords: Key words: Pseudomonas aeruginosa, flagellin, fliC, PCR

Full-Text [PDF 274 kb] [English Abstract] (7852 Downloads)

Received: 2011/08/13 | Accepted: 2014/01/12 | Published: 2014/01/12 | ePublished: 2014/01/12